Chitosan nanoparticles reduce LPS-induced inflammatory reaction via inhibition of NF-κB pathway in Caco-2 cells

Tu, Jue; Xu, Yinglei; Xu, Jianqin; Ling, Yun; Cai, Yan

doi:10.1016/j.ijbiomac.2016.02.015

Cited by 73 publications

(39 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, heparin-and chitosan-conjugated magnetic nanoparticles have shown great potential in removing LDL cholesterol from blood plasma [245]. Nanoparticles can also modulate the immune system and have been used to induce anti-inflammatory effects [246,247]. Broad-spectrum ROS scavenging nanoparticles, for example, have been utilized in mice studies to effectively decrease oxidative stress and local and systemic inflammation [248].…”

Section: Microbiota and Nanomedicine-based Approachesmentioning

confidence: 99%

“…Broad-spectrum ROS scavenging nanoparticles, for example, have been utilized in mice studies to effectively decrease oxidative stress and local and systemic inflammation [248]. Furthermore, chitosan nanoparticles induce antiinflammatory effects by decreasing the permeability of intestinal epithelial monolayer and the secretion of proinflammatory cytokines [247]. In addition, nanoparticle-based inhibitors of TLR signaling have been used to decrease inflammation and treat inflammatory diseases [249].…”

Section: Microbiota and Nanomedicine-based Approachesmentioning

confidence: 99%

See 1 more Smart Citation

Gut microbiota and cardiovascular disease: opportunities and challenges

et al. 2020

View full text Add to dashboard Cite

Coronary artery disease (CAD) is the most common health problem worldwide and remains the leading cause of morbidity and mortality. Over the past decade, it has become clear that the inhabitants of our gut, the gut microbiota, play a vital role in human metabolism, immunity, and reactions to diseases, including CAD. Although correlations have been shown between CAD and the gut microbiota, demonstration of potential causal relationships is much more complex and challenging. In this review, we will discuss the potential direct and indirect causal roots between gut microbiota and CAD development via microbial metabolites and interaction with the immune system. Uncovering the causal relationship of gut microbiota and CAD development can lead to novel microbiome-based preventative and therapeutic interventions. However, an interdisciplinary approach is required to shed light on gut bacterial-mediated mechanisms (e.g., using advanced nanomedicine technologies and incorporation of demographic factors such as age, sex, and ethnicity) to enable efficacious and high-precision preventative and therapeutic strategies for CAD. Key points The causal relationship between gut microbiota and CAD development has yet to be confirmed. It is imperative to understand the potential direct and indirect causal roots between gut microbiota and CAD development via microbial metabolites and interaction with the immune system. Dynamic elements including our diet and demographic factors such as age, sex, and ethnicity can also affect our gut microbiota and CAD development and complicate this matter. Interdisciplinary approaches are required to shed light on the factors involved in the modulation of gut microbiota and its association with CAD development. Elucidating the system-level multifaceted web of factors involved in microbiome-mediated mechanisms and human health and disease can guide novel preventative and therapeutic interventions for CAD.

show abstract

Section: Microbiota and Nanomedicine-based Approachesmentioning

confidence: 99%

Section: Microbiota and Nanomedicine-based Approachesmentioning

confidence: 99%

Gut microbiota and cardiovascular disease: opportunities and challenges

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Our previous studies showed that CNP possessed diverse biological activities, including antibacterial (Du, Xu, Xu, & Fan, 2008), antitumour (Qi, Xu, & Chen, 2007), anti-angiogenesis (Xu, Wen, & Xu, 2010) and immunological adjuvant activities (Wen, Xu, Zou, & Xu, 2011). Recently, we found that CNP could protect the Caco-2 cells, a model of human enterocytes, from LPS-induced cell membrane damage (Tu, Xu, Xu, Ling, & Cai, 2016), indicating that CNP might exhibit anti-inflammatory effects and could be used as a feed additive for weaning piglets to reduce the weaning stress.…”

Section: Introductionmentioning

confidence: 99%

Effects of chitosan nanoparticle supplementation on growth performance, humoral immunity, gut microbiota and immune responses after lipopolysaccharide challenge in weaned pigs

Mao

Yang

et al. 2019

Animal Physiology Nutrition

Self Cite

View full text Add to dashboard Cite

In this study, we aimed to determine the effects of dietary supplementation with chitosan nanoparticles (CNP) on growth performance, immune status, gut microbiota and immune responses after lipopolysaccharide challenge in weaned pigs. A total of 144 piglets were assigned to four groups receiving different dietary treatments, including basal diets supplemented with 0, 100, 200 and 400 mg/kg CNP fed for 28 days. Each treatment group included six pens (six piglets per pen). The increase in supplemental CNP concentration improved the average daily gain (ADG) and decreased the feed and gain (F/G) and diarrhoea rate (p < .05). However, significant differences in the average daily feed intake (ADFI) among different CNP concentrations were not observed. CNP also increased plasma immunoglobulin (Ig)A and IgG, and C3 and C4 concentrations in piglets in a dose‐dependent manner on day 28, whereas IgM concentration was not affected by CNP. A total of 24 piglets in the control diet and control diet with 400 mg/kg CNP supplementation groups were randomly selected for the experiment of immunological stress. Half of the pigs in each group (n = 6) were injected i.p. with Escherichia coli lipopolysaccharide (LPS) at a concentration of 100 μg/kg. The other pigs in each group were injected with sterile saline solution at the same volume. Plasma concentrations of cortisol, prostaglandin E2 (PEG2), interleukin (IL)‐6, tumour necrosis factor (TNF)‐α and IL‐1β dramatically increased after LPS challenge. However, CNP inhibited the increase in cortisol, PEG2, IL‐6 and IL‐1β levels in plasma, whereas TNF‐α level slightly increased. Moreover, the effects of CNP on the gut microbiota were also evaluated. Our results showed that dietary supplementation with CNP modified the composition of colonic microbiota, where it increased the amounts of some presumably beneficial intestinal bacteria and suppressed the growth of potential bacterial pathogens. These findings suggested CNP supplementation improved the growth performance and immune status, alleviated immunological stress and regulated intestinal ecology in weaned piglets. Based on these beneficial effects, CNP could be applied as a functional feed additives supplemented in piglets diet.

show abstract

“…LPS, the major component of the outer membrane of gram‐negative bacteria, is the main factor responsible for microglial activation, which induces septic shock syndrome and stimulates the production of pro‐inflammatory cytokines such as tumor necrosis factor alpha (TNF‐α), interleukin‐6 (IL‐6), and inflammatory mediators (Tu et al, ; Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…LPS, the major component of the outer membrane of gram-negative bacteria, is the main factor responsible for microglial activation, which induces septic shock syndrome and stimulates the production of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-a), interleukin-6 (IL-6), and inflammatory mediators (Tu et al, 2016;Wang et al, 2016). iNOS, induced by bacterial products and inflammatory cytokines in macrophages, is an enzyme that plays a key role in immune response against pathogens and is responsible for prolonged production of larger amounts of NO (Lee et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

KH‐type splicing regulatory protein mediate inflammatory response in gastric epithelial cells induced by lipopolysaccharide

Chen

Cao

et al. 2017

Cell Biology International

View full text Add to dashboard Cite

To study differential expressions of KH-type splicing regulatory protein (KSRP) and inflammatory factors and to explore the relationship between them in Lipopolysaccharide (LPS)-induced gastric epithelial cells (GES-1), cells were exposed to LPS for 24 h in the presence or absence of SC-514. Western blot and real-time PCR (RT-PCR) were used to analysis the contents of KSRP, inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). The results showed that LPS decreased the expression of KSRP protein in GES-1 cells, but not KSRP mRNA, while increasing the levels of iNOS and COX-2 proteins and mRNAs in GES-1cells. High expression of KSRP induced low expressions and stabilities of iNOS and COX-2 in GES-1 cells, indicated that KSRP protein presented negative correlation with iNOS and COX-2 with LPS stimulation. In conclusion, the regulation of expression of KSRP was mainly achieved through post-translational modification. KSRP protein participated in regulating the expression of iNOS and COX-2 in their transcription and translation levels. In response to LPS or gram negative pathogenic microorganism, KSRP could regulate Toll-like receptor (TLR)/ Nuclear factor-kappa B (NF-κB) signal pathway in GES-1 cells.

show abstract

Chitosan nanoparticles reduce LPS-induced inflammatory reaction via inhibition of NF-κB pathway in Caco-2 cells

Cited by 73 publications

References 56 publications

Gut microbiota and cardiovascular disease: opportunities and challenges

Gut microbiota and cardiovascular disease: opportunities and challenges

Effects of chitosan nanoparticle supplementation on growth performance, humoral immunity, gut microbiota and immune responses after lipopolysaccharide challenge in weaned pigs

KH‐type splicing regulatory protein mediate inflammatory response in gastric epithelial cells induced by lipopolysaccharide

Contact Info

Product

Resources

About