Chitosan-Ocimum basilicum nanocomposite as a dietary additive in Oreochromis niloticus: Effects on immune-antioxidant response, head kidney gene expression, intestinal architecture, and growth

Rahman, Afaf N. Abdel; Elshopakey, Gehad E.; Behairy, Amany; Altohamy, Dalia E.; Ahmed, Amany I.; Faroh, Khaled Yehia; Alkafafy, Mohamed; Shahin, Shimaa A.; Ibrahim, Rowida E.

doi:10.1016/j.fsi.2022.08.020

Cited by 38 publications

(9 citation statements)

References 55 publications

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“…Recent research has shown that applying nanomaterials in aquaculture fields enhances fish performance and health status [ 43 , 44 ]. In the last decades, TiO 2 NPs have been successfully applied as they own a proven toxic mechanism against bacteria [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel

Rahman,

Mahboub,

Elshopakey

et al. 2024

BMC Vet Res

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Background Pseudomonas putida is a pathogenic bacterium that induces great losses in fishes, including Nile tilapia (Oreochromis niloticus). Currently, the application of nanomaterials in aquaculture practices has gained more success as it endows promising results in therapies compared to traditional protocols. Objective Therefore, the current perspective is considered the first report to assess the anti-bacterial efficacy of titanium dioxide nanogel (TDNG) against Pseudomonas putida (P. putida) in Nile tilapia. Methods The fish (n = 200; average body weight: 47.50±1.32 g) were allocated into four random groups (control, TDNG, P. putida, and TDNG + P. putida), where 0.9 mg/L of TDNG was applied as bath treatment for ten days. Results Outcomes revealed that P. putida infection caused ethological alterations (surfacing, abnormal movement, and aggression) and depression of immune-antioxidant variables (complement 3, lysozyme activity, total antioxidant capacity, superoxide dismutase, and reduced glutathione content). Additionally, a substantial elevation in hepatorenal biomarkers (aspartate and alanine aminotransferases and creatinine) with clear histopathological changes and immuno-histochemical alterations (very weak BCL-2 and potent caspase-3 immuno-expressions) were seen. Surprisingly, treating P. putida-infected fish with TDNG improved these variables and obvious restoration of the tissue architectures. Conclusion Overall, this report encompasses the key role of TDNG as an anti-bacterial agent for controlling P. putida infection and improving the health status of Nile tilapia.

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Section: Discussionmentioning

confidence: 99%

Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel

Rahman,

Mahboub,

Elshopakey

et al. 2024

BMC Vet Res

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“…The histological characteristics of the fish intestine are of great value for nutrition studies since it is the primary location for nutrient absorption (Abdel Rahman et al, 2022; Amer et al, 2019). In this investigation, dietary inclusion of AIPH was found to improve gut morphometric represented in VW, VL and GCC in a level‐dependent way, together with preserving gut structure.…”

Section: Discussionmentioning

confidence: 99%

Using Azadirachta indica protein hydrolysate as a plant protein in Nile tilapia (Oreochromis niloticus) diet: Effects on the growth, economic efficiency, antioxidant‐immune response and resistance to Streptococcus agalactiae

Rahman

Amer

Behairy

et al. 2023

Animal Physiology Nutrition

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A feeding trial for 90 days was conducted on Nile tilapia (Oreochromis niloticus) (average weight: 25.50 ± 0.05 g) to evaluate the effect of dietary inclusion of Azadirachta indica seed protein hydrolysate (AIPH). The evaluation included the impact on the growth metrics, economic efficiency, antioxidant potential, hemato‐biochemical indices, immune response, and histological architectures. A total of 250 fish were randomly distributed in five treatments (n = 50) and received diets included with five levels of AIPH (%): 0 (control diet, AIPH0), 2 (AIPH2), 4 (AIPH4), 6 (AIPH6) or 8 (AIPH8), where AIPH partially replace fish meal by 0, 8.7%, 17.4%, 26.1%, and 34.8%, respectively. After the feeding trial, a pathogenic bacterium (Streptococcus agalactiae, 1.5 × 108 CFU/mL) was intraperitoneally injected into the fish and the survival rate was recorded. The results elucidated that AIPH‐included diets significantly (p < 0.05) enhanced the growth indices (final body weight, total feed intake, total body weight gain, and specific growth rate) and intestinal morpho‐metrics (villous width, length, muscular coat thickness, and goblet cells count) in comparison to the control diet, with the AIPH8 diet recording the highest values. Dietary AIPH inclusion significantly improved (p < 0.05) the economic efficacy indicated by reduced feed cost/kg gain and increased performance index. The fish fed on the AIPH diets had noticeably significantly higher (p < 0.05) protein profile variables (total proteins and globulin) and antioxidant capabilities (superoxide dismutase and total antioxidant capacity) than the AIPH0 group. The dietary inclusion of AIPH significantly (p < 0.05) boosted the haematological parameters (haemoglobin, packed cell volume %, and counts of red blood cells and white blood cells) and immune indices (serum bactericidal activity %, antiprotease activity, and immunoglobulin M level) in a concentration‐dependent manner. The blood glucose and malondialdehyde levels were significantly (p < 0.05) lowered by dietary AIPH (2%−8%). The albumin level and hepatorenal functioning parameters (aspartate aminotransferase, alanine aminotransferase, and creatinine) were not significantly (p > 0.05) altered by AIPH diets. Additionally, AIPH diets did not adversely alter the histology of the hepatic, renal or splenic tissues with moderately activated melano‐macrophage centres. The mortality rate among S. agalactiae‐infected fish declined as dietary AIPH levels rose, where the highest survival rate (86.67%) was found in the AIPH8 group (p < 0.05). Based on the broken line regression model, our study suggests using dietary AIPH at the optimal level of 6%. Overall, dietary AIPH inclusion enhanced the growth rate, economic efficiency, health status, and resistance of Nile tilapia to the S. agalactiae challenge. These beneficial impacts can help the aquaculture sector to be more sustainable.

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“…Abdel Rahman found that blends of animal fat and vegetable oil biodiesels can improve fuel properties and combustion characteristics. These blends also help to reduce exhaust pollutants . It was found that 2-butoxyethanol can be effectively used as a biodiesel additive to improve fuel properties and to achieve better combustion and reduced pollution.…”

Section: Biodieselmentioning

confidence: 99%

Enhancing Performance and Emission Characteristics of Biodiesel-Operated Compression Ignition Engines through Low Heat Rejection Mode and Antioxidant Additives: A Review

Rajendran,

Venkatesan,

Dhairiyasamy

et al. 2023

ACS Omega

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Depending on the heat content and compression ignition (CI) engine combustion, biodiesel is a viable substitute fuel. Biodiesel is an oxygenated, safe, sulfur-free, biodegradable, and renewable fuel. It may be utilized in CI engines in any combination with diesel fuel without requiring the engine to be significantly modified. Many research studies have been made with several biodiesels as diesel substitutes, including Pongamia pinnata, Jatropha curcas, Mangifera indica, and Madhuca longifolia. The topic of the current review is the potential of renewable fuels to outperform diesel fuel in terms of performance, combustion, and emission characteristics. In the present study, CI engines are fueled with biodiesels made from Man. indica, Mad. longifolia, and pongamia seed oil. Adopting low heat rejection (LHR) mode CI engines and adding an antioxidant agent in addition to the biodiesel blends may resolve the issue of these biodiesels’ poorer performance and increased NO emission. Both these additions may provide positive approaches in both performance and emission.

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Chitosan-Ocimum basilicum nanocomposite as a dietary additive in Oreochromis niloticus: Effects on immune-antioxidant response, head kidney gene expression, intestinal architecture, and growth

Cited by 38 publications

References 55 publications

Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel

Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel

Using Azadirachta indica protein hydrolysate as a plant protein in Nile tilapia (Oreochromis niloticus) diet: Effects on the growth, economic efficiency, antioxidant‐immune response and resistance to Streptococcus agalactiae

Enhancing Performance and Emission Characteristics of Biodiesel-Operated Compression Ignition Engines through Low Heat Rejection Mode and Antioxidant Additives: A Review

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