Chitosan@Puerarin hydrogel for accelerated wound healing in diabetic subjects by miR-29ab1 mediated inflammatory axis suppression

Zeng, Xiaoling; Chen, Baohui; Wang, Luping; Sun, Yingxiao; Jin, Zhao; Liu, Xuanyong; Ouyang, Liping; Liao, Yun

doi:10.1016/j.bioactmat.2022.04.032

Cited by 51 publications

(21 citation statements)

References 77 publications

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“…To investigate the anti-inflammatory efficacy of ZEM during the early wound repair process, IHC staining for IL-1β and IL-6 was performed. M1 macrophages are known to secrete proinflammatory cytokines such as IL-1β and IL-6 . Hence, IHC staining for IL-1β and IL-6 was widely conducted to evaluate inflammation in the wound region (Figure h,i).…”

Section: Resultsmentioning

confidence: 99%

“…M1 macrophages are known to secrete proinflammatory cytokines such as IL-1β and IL-6. 66 Hence, IHC staining for IL-1β and IL-6 was widely conducted to evaluate inflammation in the wound region (Figure 7h,i). Notably, both IL-1β and IL-6 expressions in the wound area from the ZEM-treated group significantly reduced on day 3.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Logic-Based Diagnostic and Therapeutic Nanoplatform with Infection and Inflammation Monitoring and Microenvironmental Regulation Accelerating Wound Repair

Liu

Wang

et al. 2022

ACS Appl. Mater. Interfaces

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Infectious cutaneous wounds are a thorny clinical problem. The microenvironment of the infectious wound is complicated and changes at different healing stages. Traditional treatments either have a single effect such as anti-inflammation, antibacteria, or angiogenesis or a simple mixture of several functions. They fail to deal with the change of the physiological healing process, leading to unsatisfactory outcomes. Herein, we have designed a logic-based smart nanoplatform (named as ZEM), aiming to self-monitor the wound microenvironment and accordingly react to the changes of the healing process, fitting multiple needs of physiological repair at different stages. ZEM was synthesized using zeolitic imidazolate framework-8 (ZIF-8) coated with an epigallocatechin gallate (EGCG)/Mg2+ complex. We characterized ZEM in the aspects of morphology, physical and chemical properties, and ion release pattern. At the initial stage, ZEM sensed the weakly acidic environment and responsively released a large number of zinc ions to eliminate bacterial infection. Then came the second inflammation stage, where ZEM responded to the oxidative stress of the local wound area with EGCG absorbing excessive reactive oxygen species (ROS), contributing to the downregulation of intracellular ROS. Meanwhile, local inflammation was alleviated by reducing the expression of proinflammatory M1 phenotype factors (IL-6, TNF-α, and IL-1β). Since the balance of local ROS had been achieved, the resulting disintegration of the EGCG/Mg2+ complex gave rise to the sustainable release of Mg2+ at the proliferation stage, promoting vascularized healing. In vivo animal experiments further proved the diagnostic and therapeutic functions of ZEM. All these results demonstrated that ZEM was a promising treatment strategy in soft tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Logic-Based Diagnostic and Therapeutic Nanoplatform with Infection and Inflammation Monitoring and Microenvironmental Regulation Accelerating Wound Repair

Liu

Wang

et al. 2022

ACS Appl. Mater. Interfaces

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“…The hydrogel was seen as a more suitable candidate for wound dressings because of its soft, porous and high-water content. It is beneficial for absorbing abundant exudates or blood, maintaining a comparable moist, cool and clean environment and keeping good O 2 exchange and water permeability in skin defect sites to promote wound healing and relieve patients' pain [12][13][14][15][16]. Hydrogels can be particularly useful in wound dressings since they are able to not only hydrate wounds and re-hydrate eschar but are capable of promoting autolytic debridement [17].…”

Section: Introductionmentioning

confidence: 99%

Injectable, self-healing, transparent, and antibacterial hydrogels based on chitosan and dextran for wound dressings

Nie

Wei

Sun

et al. 2023

International Journal of Biological Macromolecules

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“…The mechanism of refractory chronic DW is complex, with abnormal inflammatory response being the most important component (Sawaya et al, 2020; Theocharidis et al, 2020). Normalization of the wound immune microenvironment is a crucial strategy for accelerating wound healing in diabetes (Zeng et al, 2023). Consistent with the results of wound healing rate, the re‐epithelialization rate of the Model + PBS group was significantly slower compared to the Wild type group, whereas local application of ESO could accelerate the reepithelialization of DW (Figure 4a).…”

Section: Resultsmentioning

confidence: 99%

Sanguisorba officinalis L. promotes diabetic wound healing in rats through inflammation response mediated by macrophage

Song

Zeng

Zheng

et al. 2023

Phytotherapy Research

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Sanguisorba officinalis L., a traditional Chinese medicine, is frequently used to treat burns and scalds. But even so, it is unknown whether S. officinalis L. can accelerate diabetic wounds (DW) healing. Here, to bridge the gap, we employed in vivo and in vitro evaluations to assess the positive effect of S. officinalis L. ethanol extract (ESO) on DW. Results demonstrated that ESO dramatically improved the DW healing rate. With ESO treatment, the inappropriately elevated levels of IL6, IL1β and TNFα in DW were reduced, while the expression of IL10 was increased, indicating that the abnormal inflammation in DW was also under control. Moreover, the abnormally elevated expression of CD86 was significantly inhibited and the expression of CD206 was significantly up‐regulated following treatment with ESO. The global level of NF‐κB protein was not affected by ESO treatment, but it suppressed the expression of phosphorylated NF‐κB and prevented its nuclear entry. In addition, in RAW264.7 cells activated with lipopolysaccharide (LPS), the expression of NLRP3, Caspase1 and IL1β were significantly diminished following ESO treatment. In conclusion, ESO was proved to be a promising treatment for DW healing due to its potential to accelerate the healing process by suppressing the inflammatory response. This was achieved by increasing the ratio of M2 to M1 polarization through blocking the NF‐κB/NLRP3 signaling pathway.

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Chitosan@Puerarin hydrogel for accelerated wound healing in diabetic subjects by miR-29ab1 mediated inflammatory axis suppression

Cited by 51 publications

References 77 publications

Logic-Based Diagnostic and Therapeutic Nanoplatform with Infection and Inflammation Monitoring and Microenvironmental Regulation Accelerating Wound Repair

Logic-Based Diagnostic and Therapeutic Nanoplatform with Infection and Inflammation Monitoring and Microenvironmental Regulation Accelerating Wound Repair

Injectable, self-healing, transparent, and antibacterial hydrogels based on chitosan and dextran for wound dressings

Sanguisorba officinalis L. promotes diabetic wound healing in rats through inflammation response mediated by macrophage

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