Chitosan sulfate-lysozyme hybrid hydrogels as platforms with fine-tuned degradability and sustained inherent antibiotic and antioxidant activities

Aguanell, Antonio; Pozo, María Luisa del; Pérez-Martín, Carlos; Pontes, Gabriela Arruda Reinaux; Bastida, Ágatha; Fernández‐Mayoralas, Alfonso; García‐Junceda, Eduardo; Revuelta, Julia

doi:10.1016/j.carbpol.2022.119611

Cited by 28 publications

(19 citation statements)

References 71 publications

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“…47 Compared to the literature, there are many studies with comparable pore sizes with ours. [48][49][50] It was also seen that there are studies of wound dressings, which have reported larger pore sizes than ours. 51,52 Especially considering the pathogen penetration, the pore sizes were evaluated as favorable.…”

Section: Morphological Observationssupporting

confidence: 68%

“…Pore size also gives the ability to prevent the pathogens from permeating to and colonizing the wound dressing, and eventually penetrating the live tissue 47 . Compared to the literature, there are many studies with comparable pore sizes with ours 48–50 . It was also seen that there are studies of wound dressings, which have reported larger pore sizes than ours 51,52 .…”

Section: Resultsmentioning

confidence: 74%

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Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide

Boncukcu

Akgul

Akmayan

et al. 2022

Biotechnology Progress

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Wound healing is a process getting affected by internal and external factors and might be interrupted by infections. To overcome infections during wound healing, novel antibacterial agents such as antimicrobial peptides have gained popularity because of the rising antibiotic resistance. Therefore, in this study, a three‐dimensional polymeric scaffold was designed for the controlled release of HF‐18 peptide, with the contribution of hyaluronic acid, chondroitin sulfate, and chitosan polymers with the crosslinker genipin. The obtained scaffold structure (OPT) was found to have interconnected pores, was pH‐responsive and swelled more in acidic conditions (5446.5% at pH: 5.0). It was observed that HF‐18‐loaded OPT (P‐OPT) was able to release HF‐18 peptide both in acidic and neutral conditions in a controlled release manner. This study also demonstrated that both OPT and P‐OPT were biocompatible and promoted L929 cell attachment and migration. Antimicrobial activity assessments demonstrated that P‐OPT was effectively bactericidal on Staphylococcus aureus and methicillin‐resistant S. aureus. Moreover, OPT produced a synergistic effect on the antimicrobial activity of HF‐18 peptide, as P‐OPT showed activity below the reported MIC value. As a result, OPT is considered a promising scaffold as a carrier for HF‐18 for wound healing.

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Section: Morphological Observationssupporting

confidence: 68%

Section: Resultsmentioning

confidence: 74%

Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide

Boncukcu

Akgul

Akmayan

et al. 2022

Biotechnology Progress

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“…Diffusion is the most important release mechanism in the biopolymer–antimicrobial agent–food system (Figure ), affecting the mass transfer rate of antimicrobial active ingredients from substrates/carriers to the external environment. Diffusion often acts as a dominant role or cooperates with other patterns to regulate the release process. − It can be explained in three ways: (i) Fick’s law, , which states that the mass transfer flux of antimicrobial components is proportional to the concentration gradient; − ,− (ii) activation energy, which indicates that the molecular activation energy opens transition channels in biopolymers and facilitates the transition of diffusible substances within the pore; − (iii) free volume theory, which states that diffusion results from the redistribution of free volume triggered by the movement of polymer chain segments and is determined by the available free volume in the system. , …”

Section: Working Mechanism Of a Safp Antimicrobial Systemmentioning

confidence: 99%

“…These models will be used to investigate the release kinetic profiles of ecofriendly antimicrobial agents in food media and their potential applications in the food industry. The collected models include the zero-order (SEq 19), , first-order (SEq 20), ,,, Higuchi (SEq 21), , Korsmeyer–Peppas (SEq 11), ,,,,, and Logistic (SEq 22) ,, models, along with their specific equations, remarks, and applications (Table S1). By carefully analyzing these models in conjunction with other material testing and characterization, we can identify whether the primary release mechanism follows one of the six aforementioned patterns or involves multiple coupling mechanisms.…”

Section: Working Mechanism Of a Safp Antimicrobial Systemmentioning

confidence: 99%

Recent Advances in Sustainable Antimicrobial Food Packaging: Insights into Release Mechanisms, Design Strategies, and Applications in the Food Industry

Zhao

Zhang

et al. 2023

J. Agric. Food Chem.

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In response to the issues of foodborne microbial contamination and carbon neutrality goals, sustainable antimicrobial food packaging (SAFP) composed of renewable or biodegradable biopolymer matrices with ecofriendly antimicrobial agents has emerged. SAFP offers longer effectiveness, wider coverage, more controllability, and better environmental performance. Analyzing SAFP information, including the release profile of each antimicrobial agent for each food, the interaction of each biomass matrix with each food, the material size, form, and preparation methods, and its service quality in real foods, is crucial. While encouraging reports exist, a comprehensive review summarizing these developments is lacking. Therefore, this review critically examines recent releaseantimicrobial mechanisms, kinetics models, preparation methods, and key regulatory parameters for SAFPs based on slow-or controlled-release theory. Furthermore, it discusses fundamental physicochemical characteristics, effective concentrations, advantages, release approaches, and antimicrobial and preservative effects of various materials in food simulants or actual food. Lastly, inadequacies and future trends are explored, providing practical references to regulate the movement of active substances in different media, reduce the reliance on petrochemical-based materials, and advance food packaging and preservation technologies.

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“…Its modifications, such as carboxymethylchitosan [ 34 ], N -(2-hydroxypropyl)-3-trimethylammonium chitosan [ 35 ], chitosan sulfate [ 36 ], and chitosan acetate [ 37 ], also possess antimicrobial activity and retain good biocompatibility, mucoadhesiveness, low toxicity, and other important properties of chitosan. Moreover, chitosan sulfate–lysozyme hybrid hydrogels with fine-tuned degradability have demonstrated sustained, inherent antibiotic and antioxidant activities [ 38 ]. In creating new antimicrobial formulations, carboxymethylchitosan, N -(2-hydroxypropyl)-3-trimethylammonium chitosan, chitosan sulfate, and chitosan acetate look like promising matrices for bromelain immobilization.…”

Section: Introductionmentioning

confidence: 99%

Novel Biocatalysts Based on Bromelain Immobilized on Functionalized Chitosans and Research on Their Structural Features

et al. 2022

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Enzyme immobilization on various carriers represents an effective approach to improve their stability, reusability, and even change their catalytic properties. Here, we show the mechanism of interaction of cysteine protease bromelain with the water-soluble derivatives of chitosan—carboxymethylchitosan, N-(2-hydroxypropyl)-3-trimethylammonium chitosan, chitosan sulfate, and chitosan acetate—during immobilization and characterize the structural features and catalytic properties of obtained complexes. Chitosan sulfate and carboxymethylchitosan form the highest number of hydrogen bonds with bromelain in comparison with chitosan acetate and N-(2-hydroxypropyl)-3-trimethylammonium chitosan, leading to a higher yield of protein immobilization on chitosan sulfate and carboxymethylchitosan (up to 58 and 65%, respectively). In addition, all derivatives of chitosan studied in this work form hydrogen bonds with His158 located in the active site of bromelain (except N-(2-hydroxypropyl)-3-trimethylammonium chitosan), apparently explaining a significant decrease in the activity of biocatalysts. The N-(2-hydroxypropyl)-3-trimethylammonium chitosan displays only physical interactions with His158, thus possibly modulating the structure of the bromelain active site and leading to the hyperactivation of the enzyme, up to 208% of the total activity and 158% of the specific activity. The FTIR analysis revealed that interaction between N-(2-hydroxypropyl)-3-trimethylammonium chitosan and bromelain did not significantly change the enzyme structure. Perhaps this is due to the slowing down of aggregation and the autolysis processes during the complex formation of bromelain with a carrier, with a minimal modification of enzyme structure and its active site orientation.

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Chitosan sulfate-lysozyme hybrid hydrogels as platforms with fine-tuned degradability and sustained inherent antibiotic and antioxidant activities

Cited by 28 publications

References 71 publications

Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide

Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide

Recent Advances in Sustainable Antimicrobial Food Packaging: Insights into Release Mechanisms, Design Strategies, and Applications in the Food Industry

Novel Biocatalysts Based on Bromelain Immobilized on Functionalized Chitosans and Research on Their Structural Features

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