Chlamydiae in oviducts and uteri of repeat breeder pigs

Kauffold, Johannes; Melzer, Falk; Berndt, Angela; Hoffmann, G.; Hotzel, Helmut; Sachse, Konrad

doi:10.1016/j.theriogenology.2006.04.042

Cited by 49 publications

(53 citation statements)

References 45 publications

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“…The impact of Chlamydiaceae on animal health on pig farms is controversial because an inconsistency seems to exist between the obviously high prevalence of chlamydiae in transmission [5,15,16]. In addition, there is some data that chlamydioses in swine can be associated with enteritis, pneumonia, conjunctivitis, and pericarditis [22].…”

Section: Introductionmentioning

confidence: 99%

An experimentally inducedChlamydiasuis infection in pigs results in severe lung function disorders and pulmonary inflammation

et al. 2008

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-This study was aimed at evaluating the pathophysiology of pulmonary dysfunctions and inflammatory consequences of an acute respiratory chlamydial infection induced experimentally in conventionally raised pigs (aged 39-44 days). Eight animals were exposed to Chlamydia suis (C. suis) and four non-infected animals served as controls. The total observation period was from seven days before challenge to seven days post exposure. While non-infected control pigs did not exhibit any clinical symptoms, animals exposed to C. suis developed fever and were severely respiratory distressed within the first week after exposure. After C. suis infection, pulmonary dysfunctions were characterised by a significant decrease in the diffusion capacity of the lung (i.e. transfer factor of the lung for carbon monoxide; TL CO), a significant increase in the functional residual capacity (FRC), and significant changes in the pattern of ventilation (respiratory rate increased while the tidal volume decreased). In exhaled breath condensate (EBC), leukotriene B 4 (LTB 4 ) and interleukin 6 (IL-6) showed a tendency to increase after infection. In the broncho-alveolar lavage fluid (BALF) of C. suis infected pigs, the activity of matrix metalloprotease 9 (MMP-9) was found to be increased compared to controls. BALF cytology was characterised by increased numbers of granulocytes and activated lymphocytes. Pulmonary inflammation in infected pigs was confirmed by post mortem histology. A prominent dissemination of chlamydial bodies in the lung was accompanied by an influx of macrophages, granulocytes and activated T-cells. Data obtained in this study provide new insight into the pathogenesis of acute respiratory chlamydial infections in pigs.Chlamydia suis model / pulmonary inflammation / lung functions / exhaled breath condensate (EBC) / BALF

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Section: Introductionmentioning

confidence: 99%

An experimentally inducedChlamydiasuis infection in pigs results in severe lung function disorders and pulmonary inflammation

et al. 2008

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“…Kauffold et al (2006) psittaci, C. trachomatis e C. abortus) em porções da ampola, ístimo, junção útero-tubárica e útero, através da técnica de Reação em Cadeia de Polimerase (PCR). Diante da confirmação da infecção por C. abortus em rebanhos suinícolas no Estado de Alagoas, torna-se necessária a intensificação de pesquisas que auxiliem na determinação da distribuição do agente nas demais regiões do país e a participação dessa bactéria em falhas reprodutivas na espécie suína no Brasil.…”

Section: Resultsunclassified

“…A infecção por bactérias da família Chlamydiaceae em matrizes suínas tem sido associada a uma variedade de falhas reprodutivas, como aumento no número de fetos mumificados, abortos, mortalidade neonatal, endometrites e descargas vulvares (Thoma et al 1997, Enggemann et al 2000, Kauffold et al 2006. Para alguns autores, a infecção e a evolução da doença nos rebanhos ocorre por meio do contato com fetos abortados, restos placentários e secreções do parto em animais de qualquer idade, assim como pode ocorrer através de via venérea e do sêmen (Teankun et al 2006).…”

Section: Introductionunclassified

Prevalência e fatores de risco associados à infecção por Chlamydophila abortus em granjas suinícolas tecnificadas no Estado de Alagoas, Brasil

et al. 2011

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Objetivou-se com este estudo calcular a prevalência e identificar os fatores de risco associados à infecção por Chlamydophila abortus em suínos criados em granjas tecnificadas no Estado de Alagoas, Brasil. Para compor a amostra do estudo foram utilizados 342 suínos, sendo 312 matrizes e 30 varrões oriundos de sete granjas de ciclo completo e distribuídas em cinco municípios do Estado de Alagoas. O diagnóstico sorológico da infecção por C. abortus foi realizado através da microtécnica de Fixação do Complemento (RFC). A análise dos fatores de risco foi realizada por meio da aplicação de questionários investigativos, constituídos por perguntas objetivas referentes ao criador, às características gerais da propriedade, ao manejo produtivo, reprodutivo e sanitário. Observou-se prevalência de 10,5% (36/342) de suínos soropositivos para a infecção por C. abortus, com 85,8% das granjas analisadas com animais positivos. As variáveis que demonstraram associação significativa foram: utilização de bebedouros comuns para jovens e adultos (p=0,024; OR=10,83; IC=1,36-86,03) e método de cobertura de monta natural associada à inseminação artificial (p=0,05; OR=7,62; IC=1,00-58,31). Relata-se a primeira ocorrência de anticorpos anti-C. abortus em suínos no Brasil. Fatores como a introdução de reprodutores nos plantéis e a forma de fornecimento de água foram evidenciados como facilitadores da infecção das matrizes neste estudo. Dessa forma, medidas de controle da infecção devem ser enfocadas nesse aspecto para evitar a disseminação do agente nas granjas suinícolas e em outros plantéis da região.

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“…Recent studies indicate that these infections occur more frequently than originally thought [1][2][3][4]. In particular, the study ' Animal Chlamydiosis and its zoonotic implications' by Action 855 of the European Cooperation in Science and Technology brought the economic importance of chlamydial infections in pigs into sharp focus.…”

Section: Introductionmentioning

confidence: 99%

Novel locked nucleic acid (LNA)-based probe for the rapid identification of Chlamydia suis using real-time PCR

Lis

Kumala

Spalinski³

et al. 2014

BMC Vet Res

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Background: As the importance of chlamydial infections in pigs has become more obvious, a rapid and sensitive method to study the prevalence of Chlamydia suis in pig herds is required. Such a method should permit routine diagnostic tests for herds with clinical and subclinical infections, without the need for Chlamydia culture. Results:The main objective of this study was to develop a specific and rapid method for detecting C. suis in swine herds. A real-time PCR assay using a single locked nucleic acid (LNA)-containing probe specific for C. suis was developed based on the previously described 28S rDNA fragment used to identify Chlamydiales. Use of LNA nucleotides enabled the single probe to target a short, specific fragment of the 23S rRNA. The probe showed high specificity for C. suis and did not show any cross-reactivity with other Chlamydia or Chlamydophila species nor with swine DNA. All of the 86 tested field isolates, earlier identified as C. suis, were confirmed as positive using the newly developed assay. Conclusions: Using single LNA-based C. suis-specific probe allowed rapid and simple identification of this pathogen without requiring sequencing analysis and culturing. The proposed method may be used to study the prevalence of C. suis infection in pig herds and as a routine diagnostic test for herds with clinical and subclinical infection.

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Chlamydiae in oviducts and uteri of repeat breeder pigs

Cited by 49 publications

References 45 publications

An experimentally inducedChlamydiasuis infection in pigs results in severe lung function disorders and pulmonary inflammation

An experimentally inducedChlamydiasuis infection in pigs results in severe lung function disorders and pulmonary inflammation

Prevalência e fatores de risco associados à infecção por Chlamydophila abortus em granjas suinícolas tecnificadas no Estado de Alagoas, Brasil

Novel locked nucleic acid (LNA)-based probe for the rapid identification of Chlamydia suis using real-time PCR

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