Chlorella vulgaris modulates the expression of senescence-associated genes in replicative senescence of human diploid fibroblasts

Jaafar, Faizul; Durani, Lina Wati; Makpol, Suzana

doi:10.1007/s11033-019-05140-8

Cited by 5 publications

(9 citation statements)

References 52 publications

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“…Two types of skin cells were selected for the analysis, namely, normal human fibroblasts (BJ cells) and normal human keratinocytes (HEK cells). As extracts of different Chlorella species may act on human and rodent cells at a wide range of concentrations and the effects may also rely on solvents used [20][21][22][23][24], we decided to study both water and ethanolic extracts and the concentrations ranging from 1 to 1000 µg/mL ( Figure 1).…”

Section: The Effect Of Water and Ethanolic Extracts Of The Microalga mentioning

confidence: 99%

“…More recently, it has been observed that a hot water extract of Chlorella vulgaris at the concentrations of 400 and 600 μg/ml increased the number of viable cells of young human dermal fibroblasts (HDFs) [24]. Interestingly, the extract-mediated effect depended on replicative age of HDFs, namely, stimulatory effects in pre-senescent and senescent HDFs were noticed when 200 and 100 μg/ml water extract was used, respectively, and the concentrations ranging from 500 to 800 μg/ml decreased cell viability of senescent HDFs [24]. Water and 80% ethanolic extracts of Chlorella sorokiniana did not affect the cell viability of Chinese hamster lung fibroblasts (V79 cells) when used at the concentrations up to 80 and 20 μg/ml, respectively [23].…”

Section: The Effect Of Water and Ethanolic Extracts Of The Microalga mentioning

confidence: 99%

“…Data on Chlorella extract-associated anti-aging action are limited to selected models of replicative senescence, namely, human fibroblasts and myocytes at different passage numbers (young, pre-senescent, and senescent cells) [24,27,[43][44][45] and UV-induced cytotoxicity and senescence [46,47]. Hot water extract of Chlorella vulgaris lowered the levels of DNA damage in young, pre-senescent, and senescent fibroblasts in control conditions [27] and during oxidative stress [43].…”

Section: Attenuation Of the Development Of Stress-induced Senescence mentioning

confidence: 99%

“…However, the antioxidant activity of hot water extract of Chlorella vulgaris was limited to pre-senescent fibroblasts as judged by the extract-mediated increase in catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity [44]. More recently, Chlorella extract-mediated modulation of the expression of genes involved in oxidative stress response and DNA damage response and insulin/insulin-like growth factor-1 signaling, cell differentiation, and cell proliferation pathways was analyzed using young, pre-senescent, and senescent fibroblasts [24]. The authors concluded that Chlorella extract decreased the expression of SOD1, CAT, and copper chaperone CCS in young fibroblasts and increased the expression of SOD2 in young, pre-senescent, and senescent fibroblasts [24].…”

Section: Attenuation Of the Development Of Stress-induced Senescence mentioning

confidence: 99%

“…More recently, Chlorella extract-mediated modulation of the expression of genes involved in oxidative stress response and DNA damage response and insulin/insulin-like growth factor-1 signaling, cell differentiation, and cell proliferation pathways was analyzed using young, pre-senescent, and senescent fibroblasts [24]. The authors concluded that Chlorella extract decreased the expression of SOD1, CAT, and copper chaperone CCS in young fibroblasts and increased the expression of SOD2 in young, pre-senescent, and senescent fibroblasts [24]. Moreover, Chlorella extract promoted a decrease in mRNA levels of two regulators of cell cycle progression, namely, TP53 (p53) and CDKN2A (p16) in replicative old fibroblasts that may suggest some anti-aging effects.…”

Section: Attenuation Of the Development Of Stress-induced Senescence mentioning

confidence: 99%

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Treatment with Modified Extracts of the Microalga Planktochlorella nurekis Attenuates the Development of Stress-Induced Senescence in Human Skin Cells

Adamczyk‐Grochala

Wnuk

Duda³

et al. 2020

Nutrients

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More recently, we have proposed a safe non-vector approach to modifying the biochemical profiles of the microalga Planktochlorella nurekis and obtained twelve clones with improved content of lipids and selected pigments and B vitamins and antioxidant activity compared to unaffected cells. In the present study, the biological activity of water and ethanolic extracts of modified clones is investigated in the context of their applications in the cosmetic industry and regenerative medicine. Extract-mediated effects on cell cycle progression, proliferation, migration, mitogenic response, apoptosis induction, and oxidative and nitrosative stress promotion were analyzed in normal human fibroblasts and keratinocytes in vitro. Microalgal extracts did not promote cell proliferation and were relatively non-cytotoxic when short-term treatment was considered. Long-term stimulation with selected microalgal extracts attenuated the development of oxidative stress-induced senescence in skin cells that, at least in part, was correlated with nitric oxide signaling and increased niacin and biotin levels compared to an unmodified microalgal clone. We postulate that selected microalgal extracts of Planktochlorella nurekis can be considered to be used in skin anti-aging therapy.

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Section: The Effect Of Water and Ethanolic Extracts Of The Microalga mentioning

confidence: 99%

Section: The Effect Of Water and Ethanolic Extracts Of The Microalga mentioning

confidence: 99%

Section: Attenuation Of the Development Of Stress-induced Senescence mentioning

confidence: 99%

Section: Attenuation Of the Development Of Stress-induced Senescence mentioning

confidence: 99%

Section: Attenuation Of the Development Of Stress-induced Senescence mentioning

confidence: 99%

See 3 more Smart Citations

Treatment with Modified Extracts of the Microalga Planktochlorella nurekis Attenuates the Development of Stress-Induced Senescence in Human Skin Cells

Adamczyk‐Grochala

Wnuk

Duda³

et al. 2020

Nutrients

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Effect of Antioxidants on the Fibroblast Replicative Lifespan In Vitro

Sadowska-Bartosz

Bartosz

2020

Oxidative Medicine and Cellular Longevity

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Replicative senescence is an unalterable growth arrest of primary cells in the culture system. It has been reported that aging in vivo is related to the limited replicative capacity that normal somatic cells show in vitro. If oxidative damage contributes to the lifespan limitation, antioxidants are expected to extend the replicative lifespan of fibroblasts. This article critically reviews the results of experiments devoted to this problem performed within the last decades under conditions of in vitro culture. The results of studied are heterogeneous, some papers showing no effects of antioxidants; most finding limited enhancement of reproductive capacity of fibroblasts, some reporting a significant extension of replicative lifespan (RLS). Both natural and synthetic antioxidants were found to extend the RLS of fibroblasts, either by a direct antioxidant effect or, indirectly, by activation of signaling pathways and activation of proteasomes or hormetic effects. Most significant prolongation of RLS was reported so far for nicotinamide, N-hydroxylamines, carnosine and Methylene Blue. These results may be of importance for the design of skin-protecting cosmetics.

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Antioxidative Responses of Chlorella vulgaris Under Different Growth Phases

Yusuf

Athirah²,

Suhaila³

2022

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Chlorella vulqaris is a unicellular microorganism that offers health benefits due to its concentrated antioxidant production. This microalga has received huge attention due to its natural antioxidative property as an alternative antioxidant source because of its rapid growth, easy and flexible culture. Research to date only focuses on the growth and antioxidant production in a selected growth phase, especially exponential and stationary phases; however, so far, limited reports on the production of antioxidants in all growth phases of C. vulgaris. Thus, this study determines the growth, the enzymatic (Catalase, CAT; Ascorbate Peroxidase, APX; and guaiacol peroxidase, gPOD) specific activities and the amount of the non-enzymatic antioxidants (a-tocopherol, ascorbic acid and carotenoids) of C. vulgaris in five growth phases. Chlorella vulgaris was cultured in F/2 medium at 25±2 °C under laboratory conditions. CAT specific activities were the highest at the exponential phase (1.50±0.08 units/mg protein), whereas APX and gPOD were induced at the lag phases of 37.13±4.93 units/mg protein and 1.31±0.03 units/mg protein, respectively. The amount of a-tocopherol was accumulated at the stationary phase (97.3±4.18 µg/g.fwt), whereas the highest amount of ascorbic acid (266.67±22.22 µg/g.fwt) and carotenoids (8.16±2.52 µg/g.fwt) were at the decline phase. Production of enzymatic and non-enzymatic antioxidants in the microalgae cells indicated that they efficiently scavenged reactive oxygen species (ROS) and converted them into less harmful substances. In addition, the production of these antioxidants in different growth phases can be used as a guideline to produce massive antioxidants, which can be commercialized in the food and pharmaceutical industries.

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Chlorella vulgaris modulates the expression of senescence-associated genes in replicative senescence of human diploid fibroblasts

Cited by 5 publications

References 52 publications

Treatment with Modified Extracts of the Microalga Planktochlorella nurekis Attenuates the Development of Stress-Induced Senescence in Human Skin Cells

Treatment with Modified Extracts of the Microalga Planktochlorella nurekis Attenuates the Development of Stress-Induced Senescence in Human Skin Cells

Effect of Antioxidants on the Fibroblast Replicative Lifespan In Vitro

Antioxidative Responses of Chlorella vulgaris Under Different Growth Phases

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