Chlorhexidine-loaded functionalized mesoporous MCM-41 poly(methylmethacrylate) based composites with Candida antibiofilm activity

Abstract

The complete or partial dentures made of acrylic resins or composites constitute the support for Candida biofilm with consequent onset of stomatitis and candidiasis. In this paper polymethylmethacrylate composites containing chlorhexidine are prepared. The drug was loaded inside the mesopores of the silicate MCM-41 which had been properly silanized with an acrylic coupling agent. Four different chlorhexidine loaded composites were prepared using MCM-41 silanized with four different amounts of 3-methacryloxypropyl-trimethoxisilane. The composites prepared showed a decreased resin polymerization degree in comparison to the pure resin. Anyway the composite obtained with MCM-41 with the lowest silanization degree, showed a good polymerization degree, higher than that obtained when free chlorhexidine was added to the resin. This composite was able to decrease Candida biofilm adhesion and formation and inhibitedCandida biofilm proliferation for the duration of the test (120 days).

Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts

“…Various mesoporous structures have been developed for the delivery of ions and drugs with applications in dentistry. Ambrogi et al [ 27 ] loaded chlorhexidine on functionalized mesoporous MCM-41 nanoparticles and prepared poly(methylmethacrylate) based composites with Candida antibiofilm activity, while Yan et al [ 28 ] used chlorhexidine-encapsulated mesoporous silica as a modifier of a commercial dental adhesive. Lu et al [ 29 ] reported that the dual-controlled release of chlorhexidine and silver ions from biodegradable mesoporous silica nanoparticles was effective against S. mutans, suggesting their potential use as nanoantiseptics for oral biofilm therapies.…”

Resin-Based Materials with Chlorhexidine-Loaded MCM-41: Surface Characteristics, Drug Release, and Antibiofilm Activity

“…MCM-41 was properly silanized and the formation of silanized MCM-41 was verified by FT-IR spectroscopy. FT-IR spectrum (Figure S1) of silanized MCM-41 revealed a band at 1636 cm –1 due to the stretching vibration of the CC bond in γ-MPS and a band at 1702 cm –1 , relative to the CO stretching frequency.…”

“…Samples were prepared by grounding the specimen with KBr and spectra were obtained with 100 scans at a resolution of 1 cm –1 . To determinate the percentage of unreacted double bonds, the absorbance intensities of the methacrylate CC peak at 1640 cm –1 and of the unchanging CO peak from the ester group at 1720 cm –1 were calculated before and after polymerization ,, using a baseline method. The DC was calculated using the following formula: Where P 1 is the peak area of CC methacrylate peak and P 2 is the peak area relative to the CO of the ester group.…”

“…Mesoporous silicates, whose main examples are MCM-41 and SBA-15, are ordered porous materials with high specific surface area and unidirectional pores, able to host drugs inside the mesopores and to release them in a modified manner. − Because of their good properties, they have found numerous applications in the biomedical field . To the best of our knowledge, only a few papers , proposed them as carriers for entrapment of chlorhexidine (CHX) in dental materials with the hypothesis that, once encapsulated inside the pores, the drug would not interfere with the polymerization process and with physical and mechanical properties of the final material. Zhang et al .…”

“…On the bases of the good results reported in our previous work, with the aim of increasing the knowledge on the drug release and surface phenomena and their relationship with antibiofilm activity, in this paper, an acrylic resin containing CHX-loaded spherical and narrow size silanized MCM-41 was prepared. The spherical and narrow shape was chosen as it improves general properties of composites …”