Chloroform extract of aged black garlic attenuates TNF‐α‐induced ROS generation, VCAM‐1 expression, NF‐κB activation and adhesiveness for monocytes in human umbilical vein endothelial cells

Abstract

Aged black garlic is a type of fermented garlic (Allium sativum) which has been used in Oriental countries for a long time because of various biological properties of garlic derivatives. The current study explored the potential of the chloroform extract of aged black garlic (CEABG) in attenuating the activities of adhesion molecules in tumor necrosis factor-α (TNF-α)-stimulated human umbilical vein endothelial cells (HUVECs). The study was performed on HUVECs that were pretreated with 30 μg/mL of CEABG before TNF-α treatment. Treatment of HUVECs with CEABG significantly inhibited TNF-α-induced reactive oxygen species (ROS) formation. HUVECs treated with CEABG showed markedly suppressed TNF-α-induced mRNA expression of VCAM-1, but little alteration in ICAM-1 and E-selectin mRNA expression. CEABG treatment also significantly decreased the TNF-α-induced cell surface and total protein expression of VCAM-1 without affecting ICAM-1 and E-selectin expression. In addition, treatment of HUVECs with CEABG markedly reduced THP-1 monocyte adhesion to TNF-α-stimulated HUVECs. Furthermore, CEABG significantly inhibited NF-κB transcription factor activation in TNF-α-stimulated HUVECs. The data provide new evidence of the antiinflammatory properties of CEABG that may have a potential therapeutic use for the prevention and treatment of vascular diseases such as atherosclerosis through mechanisms involving the inhibition of VCAM-1 expression and NF-κB activation in vascular endothelial cells.

Astragalus polysaccharides suppress ICAM-1 and VCAM-1 expression in TNF-α-treated human vascular endothelial cells by blocking NF-κB activation

“…Atherosclerosis is associated with increased intracellular oxidative stress [18] . Oxidative stress can lead to adhesion function damage of HUVECs.…”

Garlic Extract Ameliorates Renal and Cardiopulmonary Injury in the Rats with Chronic Renal Failure

“…Recently, in human umbilical vein endothelial cells, garlic was shown to attenuate TNF-α-induced ROS generation, VCAM-1 expression, NF-κB activation, and adhesiveness for monocytes. 64 In conclusion, 3-week garlic supplementation attenuated oxidative injury in both the renal and cardiopulmonary tissues while the renal functions were improved. These observations point to the potential role of garlic supplementation as an adjunct in the therapeutic strategies aimed at retarding chronic kidney disease progression and its cardiopulmonary complications.…”

Allium sativum

“…Studies by Lee et al ( 2011 ) showed that aged black garlic possessed anti-infl ammatory activity and may have a potential therapeutic use for the prevention and treatment of vascular diseases such as atherosclerosis through mechanisms involving the inhibition of VCAM-1 expression and NF-κB activation in vascular endothelial cells. The chloroform extract of aged black garlic signifi cantly inhibited TNF-α-induced reactive oxygen species (ROS) formation and suppressed TNF-α-induced mRNA expression of VCAM-1 in human umbilical vein endothelial cells (HUVECs).…”