Chlorogenic acid attenuates virulence factors and pathogenicity of Pseudomonas aeruginosa by regulating quorum sensing

Wang, Hong; Chu, Weihua; Ye, Chao; Gaëta, Bruno; Tao, Huimin; Wang, Min; Zheng, Qiuju

doi:10.1007/s00253-018-9482-7

Cited by 75 publications

(55 citation statements)

References 50 publications

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“…[96] The structurally related chlorogenic acid only slightly inhibited swarming of P. aeruginosa but also exhibitedg lobal effects on quorum-sensing-controlled virulence factors. [97] The compound (Z,Z)-5-(trideca-4',7'-dienyl)-resorcinol that was isolated from the plant Lithream olleoides significantly inhibited swarming motility of P. mirabilis at 28 mm and completely abolished swarming at 433 mm. [98] Furthermore, many similarp lantderived phenolic compounds including caffeica cid, cinnamic acid, ferulica cid, and vanillic acid have been reported to inhibit swarming of P. aeruginosa at 4mm.…”

Section: Secondary Plant Metabolitesmentioning

confidence: 99%

Inhibitors of Bacterial Swarming Behavior

Rütschlin

Böttcher

2019

Chemistry A European J

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Bacteria can migrate in groups of flagella‐driven cells over semisolid surfaces. This coordinated form of motility is called swarming behavior. Swarming is associated with enhanced virulence and antibiotic resistance of various human pathogens and may be considered as favorable adaptation to the diverse challenges that microbes face in rapidly changing environments. Consequently, the differentiation of motile swarmer cells is tightly regulated and involves multi‐layered signaling networks. Controlling swarming behavior is of major interest for the development of novel anti‐infective strategies. In addition, compounds that block swarming represent important tools for more detailed insights into the molecular mechanisms of the coordination of bacterial population behavior. Over the past decades, there has been major progress in the discovery of small‐molecule modulators and mechanisms that allow selective inhibition of swarming behavior. Herein, an overview of the achievements in the field and future directions and challenges will be presented.

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Section: Secondary Plant Metabolitesmentioning

confidence: 99%

Inhibitors of Bacterial Swarming Behavior

Rütschlin

Böttcher

2019

Chemistry A European J

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“…A recent study [ 16 ] showed that tri-CQA was more active than di-CQA against penicillin sensitive and resistant S. aureus strains, as well as methicillin-resistant S. aureus . While the effect of CQA on the virulence factors and pathogenicity of P. aeruginosa is well understood [ 44 , 45 ], the antibacterial potential of di-CQA against P. aeruginosa strains is controversial and requires further investigation. A number of studies have shown a lack or a weak antibacterial activity of this metabolite against P. aeruginosa [ 46 , 47 ], however, others studies have found approximately MIC values similar to ours of di-CQA against P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-diCQA Accumulation, a Potent Antioxidant and Antibacterial Molecule

et al. 2020

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Cichorium intybus L. (Asteraceae) is an important industrial crop, as well as a medicinal plant which produces some bioactive compounds implicated in various biological effects with potential applications in human health. Particularly, roots produce hydroxycinnamic acids like 5-caffeoyquinic acid and 3,5-dicaffeoylquinic acid (di-CQA). The present investigation relates to the use of methyl jasmonate for enhancing phenolic compounds accumulation and production in hairy root cultures of C. intybus. Elicitated hairy root growth rate increased 13.3 times compared with the initial inoculum in a period of 14 days and di-CQA production represented about 12% of DW. The elicitation has also promoted the production of tricaffeoylquinic acid never described in the chicory roots and identified as 3,4,5-tricaffeoyquinic acid by means of nuclear magnetic resonance. Our study confirmed the strong anti-oxidant effect of di-CQA. Our results also confirmed globally a selectivity of action of di-CQA against Gram-positive bacteria, in particular against some strains of Staphylococcus and Streptococcus. However, a non-negligible antibacterial activity of di-CQA against Pseudomonas aeruginosa was also underlined (MIC = 0.156 mg.mL−1 against some P. aeruginosa strains). The influence of di-CQA has been explored to evaluate its impact on the physiology of P. aeruginosa. Di-CQA showed no effect on the biofilm formation and the production of extracellular pyocyanin. However, it demonstrated an effect on virulence through the production of pyoverdine with a dose-dependent manner by more than 7-fold when treated at a concentration of 128 µg·mL−1, thus suggesting a link between di-CQA and iron sequestration. This study shows that elicitated hairy root cultures of chicory can be developed for the production of di-CQA, a secondary metabolite with high antibacterial potential.

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“…The effects of glucose on the growth rate of P. aeruginosa strains were determined according to a method described previously, with some modifications [24]. Briefly, overnight cultures were diluted 1:100 into fresh LB broth and incubated at 37°C for 4 h before adjusting the OD 600 values to 0.1.…”

Section: Growth Curve Assaymentioning

confidence: 99%

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

Chen

et al. 2020

BMC Microbiol

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Background: Pseudomonas aeruginosa is the most common Gram-negative pathogen responsible for chronic wound infections, such as diabetic foot infections, and further exacerbates the treatment options and cost of such conditions. Hypertonic glucose, a commonly used prolotherapy solution, can accelerate the proliferation of granulation tissue and improve microcirculation in wounds. However, the action of hypertonic glucose on bacterial pathogens that infect wounds is unclear. In this study, we investigated the inhibitory effects of hypertonic glucose on multidrug-resistant P. aeruginosa strains isolated from diabetic foot infections. Hypertonic glucose represents a novel approach to control chronic wound infections caused by P. aeruginosa. Results: Four multidrug-resistant P. aeruginosa clinical strains isolated from diabetic foot ulcers from a tertiary hospital in China and the reference P. aeruginosa PAO1 strain were studied. Hypertonic glucose significantly inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa clinical strains and PAO1. Furthermore, hypertonic glucose significantly reduced the production of pyocyanin and elastase virulence factors in P. aeruginosa. The expression of major quorum sensing genes (lasI, lasR, rhlI, and rhlR) in P. aeruginosa were all downregulated in response to hypertonic glucose treatment. In a Galleria mellonella larvae infection model, the administration of hypertonic glucose was shown to increase the survival rates of larvae infected by P. aeruginosa strains (3/5). Conclusions: Hypertonic glucose inhibited the growth, biofilm formation, and swimming motility of P. aeruginosa, as well as reduced the production of virulence factors and quorum sensing gene expression. Further studies that investigate hypertonic glucose therapy should be considered in treating chronic wound infections.

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Chlorogenic acid attenuates virulence factors and pathogenicity of Pseudomonas aeruginosa by regulating quorum sensing

Cited by 75 publications

References 50 publications

Inhibitors of Bacterial Swarming Behavior

Inhibitors of Bacterial Swarming Behavior

MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-diCQA Accumulation, a Potent Antioxidant and Antibacterial Molecule

Hypertonic glucose inhibits growth and attenuates virulence factors of multidrug-resistant Pseudomonas aeruginosa

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