Chlorophyll biosynthesis and transcriptome profiles of chlorophyll b-deficient type 2b rice (Oryza sativa L.)

Nguyen, Minh Khiem; Yang, Chi-Ming; Shih, Tin-Han; Lin, Szu-Hsien; Pham, Giang Tuyet; Nguyen, Hoang Chinh

doi:10.15835/nbha49312380

Cited by 5 publications

(4 citation statements)

References 66 publications

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“…The OsHSF genes also co-expressed with Golden 2-like family genes (G2-like) that have been characterized by regulating the formation of chloroplasts during the transition and early maturing phases Fig. 14 [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

In-silico analysis of heat shock transcription factor (OsHSF) gene family in rice (Oryza sativa L.)

Shamshad,

Rashid,

Zaman

2023

BMC Plant Biol

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Background One of the most important cash crops worldwide is rice (Oryza sativa L.). Under varying climatic conditions, however, its yield is negatively affected. In order to create rice varieties that are resilient to abiotic stress, it is essential to explore the factors that control rice growth, development, and are source of resistance. HSFs (heat shock transcription factors) control a variety of plant biological processes and responses to environmental stress. The in-silico analysis offers a platform for thorough genome-wide identification of OsHSF genes in the rice genome. Results In this study, 25 randomly dispersed HSF genes with significant DNA binding domains (DBD) were found in the rice genome. According to a gene structural analysis, all members of the OsHSF family share Gly-66, Phe-67, Lys-69, Trp-75, Glu-76, Phe-77, Ala-78, Phe-82, Ile-93, and Arg-96. Rice HSF family genes are widely distributed in the vegetative organs, first in the roots and then in the leaf and stem; in contrast, in reproductive tissues, the embryo and lemma exhibit the highest levels of gene expression. According to chromosomal localization, tandem duplication and repetition may have aided in the development of novel genes in the rice genome. OsHSFs have a significant role in the regulation of gene expression, regulation in primary metabolism and tolerance to environmental stress, according to gene networking analyses. Conclusion Six genes viz; Os01g39020, Os01g53220, Os03g25080, Os01g54550, Os02g13800 and Os10g28340 were annotated as promising genes. This study provides novel insights for functional studies on the OsHSFs in rice breeding programs. With the ultimate goal of enhancing crops, the data collected in this survey will be valuable for performing genomic research to pinpoint the specific function of the HSF gene during stress responses.

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Section: Discussionmentioning

confidence: 99%

In-silico analysis of heat shock transcription factor (OsHSF) gene family in rice (Oryza sativa L.)

Shamshad,

Rashid,

Zaman

2023

BMC Plant Biol

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“…Secondarily, chlorophylls can also work as antioxidants [62]. CHB is considered an accessory to CHA [57]. In this experiment, CHB in inoculated plants was enhanced after 6 and 8 weeks, but CHA was only significantly affected after 8 weeks, with increases post-inoculation.…”

Section: Discussionmentioning

confidence: 77%

“…In various studies, plant photosynthesis pigment content can be linked to the general health and vigor of the plant [10,[57][58][59]. However, CHA, CHB and CAR have secondary functions within plants.…”

Section: Discussionmentioning

confidence: 99%

Populus tremula × P. alba Microshoot Secondary Metabolism Response after Paenibacillus sp. Inoculation In Vitro

Vaitiekūnaite

Striganavičiūtė²,

Mishcherikova³

et al. 2022

Forests

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Bacterial biostimulants are an eco-friendly alternative to chemical fertilizers. However, before their introduction into open ecosystems, broad-scope studies need to be carried out. Paenibacillus sp. was shown to positively affect poplar root growth. It was hypothesized that alongside these improvements, the Paenibacillus sp. inoculant may affect its host’s secondary metabolism. Populus tremula × P. alba microshoots were inoculated in vitro. Microshoots were tested for chlorophyll, carotenoid, total flavonoid (TFC), total phenol content (TPC) and free radical scavenging capacity during primary growth after 4, 6 and 8 weeks. The results showed that the inoculation decreased shoot phenolics and free radical scavenging capacity after 6 and 8 weeks. Chlorophyll b amounts increased after 6 and 8 weeks. Carotenoid content decreased after 6 weeks, while chlorophyll a and carotenoid levels increased after 8 weeks. Correlation and principal component analyses showed that the inoculant changed the way in which the photosynthesis pigment content relates to TPC, TFC and radical scavenging activity. Overall, these data suggest that the inoculant does statistically significantly affect Populus tree secondary metabolism in the later stages of the initial growth period. This effect may potentially be compensatory in nature.

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“…The rice leaf color phenotype is mostly controlled by a pair of recessive nuclear genes, which are among more than 150 genes related to chlorophyll content contained in the Gramene database (http://www.gramene.org/ (accessed on 15 February 2023)). These genes directly or indirectly regulate chlorophyll metabolism, photosynthesis, carbon fixation, energy metabolism, ribosome metabolism, post-translational modification, and anthocyanin biosynthesis, which affect leaf color changes in a variety of ways [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Chloroplast Damage and Photosynthetic System Disorder Induced Chlorosis in the Leaves of Rice Seedlings under Excessive Biuret

Zhang,

Chen,

Zhang

et al. 2023

Agronomy

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Excessive biuret in fertilizer causes leaf albinism in direct-seeded rice fields. This study aimed to provide a comprehensive understanding of the underlying physiology and molecular mechanisms of leaf chlorosis via biuret using morphophysiological and transcriptome analyses. The induction of biuret in albino rice leaves was examined in a net-growing cultivation bed. Some key morphophysiological indices were measured including biuret content, blade ultrastructure, chlorophyll content, and chlorophyll fluorescence parameters. Candidate genes in the chlorotic leaves under biuret stress were also excavated using transcriptome analysis. Furthermore, physiological and biochemical analyses of the changes in enzyme activities and intermediate metabolite contents in relation to the phenotypic changes in the leaves were carried out. The chlorotic leaves of rice seedlings showed higher biuret accumulation, and the leaves suffered severe damage with higher malondialdehyde contents and low chlorophyll contents. Abnormal chloroplast ultrastructures and thylakoid membrane structure loss were observed in chlorotic leaves under biuret exposure. The related genes involved in the chloroplast development, photosynthesis (including antenna proteins), and carbon fixation pathways were significantly downregulated, which suggests that photosynthesis was destroyed in the chlorotic leaves of rice seedlings. Biuret disturbed the photosynthetic system in chloroplast thylakoid membranes by inhibiting chloroplast development, thereby promoting the formation of the chlorotic leaf phenotype in rice seedlings. Our results promote the understanding of the molecular mechanism of rice in response to biuret toxicity.

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Chlorophyll biosynthesis and transcriptome profiles of chlorophyll b-deficient type 2b rice (Oryza sativa L.)

Cited by 5 publications

References 66 publications

In-silico analysis of heat shock transcription factor (OsHSF) gene family in rice (Oryza sativa L.)

In-silico analysis of heat shock transcription factor (OsHSF) gene family in rice (Oryza sativa L.)

Populus tremula × P. alba Microshoot Secondary Metabolism Response after Paenibacillus sp. Inoculation In Vitro

Chloroplast Damage and Photosynthetic System Disorder Induced Chlorosis in the Leaves of Rice Seedlings under Excessive Biuret

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