Chlorophyll fluorescence – A tool to assess photosynthetic performance and stress photophysiology in symbiotic marine invertebrates and seaplants

Bhagooli, Ranjeet; Mattan-Moorgawa, Sushma; Kaullysing, Deepeeka; Louis, Yohan Didier; Gopeechund, Arvind; Ramah, Sundy; Soondur, Mouneshwar; Pilly, Sivajyodee Sannassy; Beesoo, Rima; Wijayanti, Diah Permata; Bachok, Zainudin; Monrás, Víctor Cubillos; Casareto, Beatriz E.; Suzuki, Yuzuru; Baker, Andrew C.

doi:10.1016/j.marpolbul.2021.112059

Cited by 67 publications

(55 citation statements)

References 213 publications

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“…A more prominent heat stress effects on growth rates than on photosynthesis was also reported in other tropical species (Sinutok et al, 2012;Terada et al, 2016), including P. acuta, and T. hemprichii (George et al, 2018;Poquita-Du et al, 2020;Ho et al, 2021). This implies that the general notion that photosynthesis is one of the most sensitive physiological processes (Bhagooli et al, 2021) and that its response may precede the response at growth level may not entirely hold true for heat stress or could not be supported by the chlorophyll fluorescence parameters measured in the present study. Tropical shallow-water organisms may be able to maintain an intact photosynthetic machinery during warming events, albeit slower biomass production, which may persist in some species after the warming period is over (e.g., H. ovalis; P. boryana, and U. intestinalis at 37 • C).…”

Section: Effects Of Warming On Overall Performance Of Shallow-water Marine Organismscontrasting

confidence: 60%

Experimental Assessment of Vulnerability to Warming in Tropical Shallow-Water Marine Organisms

et al. 2021

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Tropical shallow-water habitats represent the marine environments with the greatest biodiversity; however, these habitats are the most vulnerable to climate warming. Corals, seagrasses, and macroalgae play a crucial role in the structure, functions, and processes of the coastal ecosystems. Understanding their growth and physiological responses to elevated temperature and interspecific sensitivity is a necessary step to predict the fate of future coastal community. Six species representatives, including Pocillopora acuta, Porites lutea, Halophila ovalis, Thalassia hemprichii, Padina boryana, and Ulva intestinalis, collected from Phuket, Thailand, were subjected to stress manipulation for 5 days. Corals were tested at 27, 29.5, 32, and 34.5°C, while seagrasses and macroalgae were tested at 27, 32, 37, and 42°C. After the stress period, the species were allowed to recover for 5 days at 27°C for corals and 32°C for seagrasses and macroalgae. Non-destructive evaluation of photosynthetic parameters (Fv/Fm, Fv/F0, ϕPSII and rapid light curves) was carried out on days 0, 3, 5, 6, 8, and 10. Chlorophyll contents and growth rates were quantified at the end of stress, and recovery periods. An integrated biomarker response (IBR) approach was adopted to integrate the candidate responses (Fv/Fm, chlorophyll content, and growth rate) and quantify the overall temperature effects. Elevated temperatures were found to affect photosynthesis, chlorophyll content, and growth rates of all species. Lethal effects were detected at 34.5°C in corals, whereas adverse but recoverable effects were detected at 32°C. Seagrasses and macroalgae displayed a rapid decline in photosynthesis and lethal effects at 42°C. In some species, sublethal stress manifested as slower growth and lower chlorophyll content at 37°C, while photosynthesis remained unaffected. Among all, T. hemprichii displayed the highest thermotolerance. IBR provided evidence that elevated temperature affected the overall performance of all tested species, depending on temperature level. Our findings show a sensitivity that differs among important groups of tropical marine organisms inhabiting the same shallow-water environments and highlights the importance of integrating biomarkers across biological levels to assess their vulnerability to climate warming.

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Section: Effects Of Warming On Overall Performance Of Shallow-water Marine Organismscontrasting

confidence: 60%

Experimental Assessment of Vulnerability to Warming in Tropical Shallow-Water Marine Organisms

et al. 2021

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“…Chlorophyll fluorescence can be used to detect the real photosynthetic behavior of the whole plant under stress quickly ( Bhagooli et al, 2021 ). Based on this, it is possible to evaluate both the function of photosynthetic apparatus and the effects of environmental stress on plants.…”

Section: Discussionmentioning

confidence: 99%

Effect of precipitation change on the photosynthetic performance of Phragmites australis under elevated temperature conditions

Teng

Liu

Chu

et al. 2022

PeerJ

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Background As a fundamental metabolism, leaf photosynthesis not only provides necessary energy for plant survival and growth but also plays an important role in global carbon fixation. However, photosynthesis is highly susceptible to environmental stresses and can be significantly influenced by future climate change. Methods In this study, we examined the photosynthetic responses of Phragmites australis (P. australis) to three precipitation treatments (control, decreased 30%, and increased 30%) under two thermal regimes (ambient temperature and +4 °C) in environment-controlled chambers. Results Our results showed that the net CO2 assimilation rate (Pn), maximal rate of Rubisco (Vcmax), maximal rate of ribulose-bisphosphate (RuBP) regeneration (Jmax) and chlorophyll (Chl) content were enhanced under increased precipitation condition, but were declined drastically under the condition of water deficit. The increased precipitation had no significant effect on malondialdehyde (MDA) content (p > 0.05), but water deficit drastically enhanced the MDA content by 10.1%. Meanwhile, a high temperature inhibited the positive effects of increased precipitation, aggravated the adverse effects of drought. The combination of high temperature and water deficit had more detrimental effect on P. australis than a single factor. Moreover, non-stomatal limitation caused by precipitation change played a major role in determining carbon assimilation rate. Under ambient temperature, Chl content had close relationship with Pn (R2 = 0.86, p < 0.01). Under high temperature, Pn was ralated to MDA content (R2 = 0.81, p < 0.01). High temperature disrupted the balance between Vcmax and Jmax (the ratio of Jmax to Vcmax decreased from 1.88 to 1.12) which resulted in a negative effect on the photosynthesis of P. australis. Furthermore, by the analysis of Chl fluorescence, we found that the xanthophyll cycle-mediated thermal dissipation played a major role in PSII photoprotection, resulting in no significant change on actual PSII quantum yield (ΦPSII) under both changing precipitation and high temperature conditions. Conclusions Our results highlight the significant role of precipitation change in regulating the photosynthetic performance of P. australis under elevated temperature conditions, which may exacerbate the drought-induced primary productivity reduction of P. australis under future climate scenarios.

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“…Rapid light curves (RLCs) generated by Pulse-Amplitude-Modulated (PAM) fl uorometry (Beer and Bjork, 2000;Beer and Axelsson, 2004;Campbell et al, 2008) provide chlorophyll a fl uorescence parameters such as eff ective quantum yield at photosystem II (F PSII ), initial slope (a), photoinhibition (b), maximum relative electron transport rate (rETR m ), and maximum non-photochemical quenching (NPQmax ). These act as a proxy indicating natural variations and stress responses in marine plants including seagrasses and macroalgae (Li et al, 2010(Li et al, , 2014Bhagooli et al, 2021). Diving-PAM fl uorometry is a non-destructive method to estimate photosynthetic activities in seaplants (Beer et al, 2001) and symbiotic marine invertebrates (Bhagooli et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Variable photo-physiological performance of macroalgae and seagrasses from Saya de Malha and Nazareth Banks, Mascarene Plateau

Bhagooli

Soondur

Ramah

et al. 2022

West. Ind. Oc. J. Mar. Sci.

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The photosynthetic performance of macroalgae and seagrasses related to their body parts, depth and colours from the poorly-studied Saya de Malha and Nazareth Banks on the Mascarene Plateau was investigated in this study. Two seagrass (Thalassodendron ciliatum and Halophila decipiens) and seven macroalgae species (Caulerpa cupressoides, Acrosorium ciliolatum, Dictyosphaeria cavernosa, Halimeda opuntia, Ulva sp., Udotea orientalis and Udotea palmetta) were collected using the five Van Veen grabs attached to the Video-Assisted Multi-Sampler (VAMS) from 29-79 m depths in May during the FAO EAF-Nansen Research Programme 2018. The photosynthetic performance was measured using a Diving-Pulse-Amplitude-Modulated (D-PAM) fluorometer and the parameters included effective quantum yield at photosystem II (PSII) (ΦPSII), relative maximum electron transport rate (rETRm), photosynthetic efficiency (α), photoinhibition (β), saturating light level (Ek), and maximum non-photochemical quenching (NPQmax). All photo-physiological parameters varied significantly in T. ciliatum and C. cupressoides across their body parts. However, variation with seawater depths was not significant for NPQmax and β in the seagrass, and ΦPSII, rETRm and β in the macroalgae. Photo-physiological functioning of the leaf of T. ciliatum was optimal at 40 m. The photosynthetic performance of the frond and stolon of C. cupressoides decreased and remained unchanged, respectively, at 79 m when compared to that at 29 m. The whitish lobes of H. opuntia at 31 m exhibited significantly lower photosynthetic performance, in terms of ΦPSII, rETRm,α and Ek, than the greenish lobes. These findings provide a first insight of seaplant body parts-, depth- and colour-related photo-physiological performance from the Mascarene Plateau.

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Chlorophyll fluorescence – A tool to assess photosynthetic performance and stress photophysiology in symbiotic marine invertebrates and seaplants

Cited by 67 publications

References 213 publications

Experimental Assessment of Vulnerability to Warming in Tropical Shallow-Water Marine Organisms

Experimental Assessment of Vulnerability to Warming in Tropical Shallow-Water Marine Organisms

Effect of precipitation change on the photosynthetic performance of Phragmites australis under elevated temperature conditions

Variable photo-physiological performance of macroalgae and seagrasses from Saya de Malha and Nazareth Banks, Mascarene Plateau

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