Chlorophyll rich biomolecular fraction of A. cadamba loaded into polymeric nanosystem coupled with Photothermal Therapy: A synergistic approach for cancer theranostics

Pemmaraju, Deepak Bharadwaj; Appidi, Tejaswini; Minhas, Gillipsie; Singh, Surya Prakash; Khan, Nooruddin; Pal, Mahadeb; Srivastava, Rohit; Rengan, Aravind Kumar

doi:10.1016/j.ijbiomac.2017.09.084

Cited by 48 publications

(36 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…leaf and its polymeric nanoparticles showed significant anticancer activity against different cancerous cell lines [50]. Another polymeric nanoparticle prepared from the plant Anthocephalus cadamba containing chlorophyll fraction showed cytotoxicity activity in skin cancerous cell line [51].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and Anticancer Activity of Extract and Fractions Obtained From Diospyros Melanoxylon Roxb. Leaves and Correlation With Their Polyphenolic Profiles

Rashid

Majumder

et al. 2018

Int J Pharm Pharm Sci

Self Cite

View full text Add to dashboard Cite

Objective: The current study evaluates the antioxidant and cytotoxic prospective of the leaves from Diospyros melanoxylon Roxb. (D. melanoxylon).Methods: Qualitative phytochemical analysis of the samples from D. melanoxylon was carried out for the detection of secondary metabolites. Total content of flavonoids, phenolics, triterpenoids, and tannins in D. melanoxylon was evaluated using colorimetric assay. Qualitative analysis of polyphenolic compounds was performed using HPLC method. The antioxidant activity was examined by assessing the various free radical scavenging assays. Five human cancerous cell lines (HeLa, MCF-7, HCT-116, PC-3, and HEK293), one rat colon cancer cell line (RCC 45) and two normal cell lines (NKE and WI-38) were used for evaluating the anticancer activity through methylene blue assay.Results: The results indicated that ethyl acetate fraction (EAF) and aqueous fraction (AQF) exhibited the remarkable content of flavonoids, phenolics, triterpenoids, and tannins. Both EAF and AQF have cytotoxic activity with significant IC50 values but not showed significant antioxidant activity. However, the most powerful anticancer activity was recorded by EAF followed by AQF in a dose-dependent manner. It was also revealed that EAF and AQF were biocompatible with the normal cell lines.Conclusion: Our principal finding exhibited a potent anticancer activity. Furthermore, it was also indicated that samples contain secondary metabolites like flavonoids, phenolics, triterpenoids, and tannins. We suggest that the further investigation on this herb is effective against various types of ailments including cancer.Keywords: Diospyros melanoxylon Roxb., Scanning Electron Microscopy (SEM), High-Pressure Liquid Chromatography (HPLC), Polyphenols, Antioxidant, Anticancer

show abstract

Section: Discussionmentioning

confidence: 99%

Antioxidant and Anticancer Activity of Extract and Fractions Obtained From Diospyros Melanoxylon Roxb. Leaves and Correlation With Their Polyphenolic Profiles

Rashid

Majumder

et al. 2018

Int J Pharm Pharm Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, it has been used in cosmetology (e.g., against acne vulgaris [4]). This biomolecule possesses anti-cancer [5][6][7][8], anti-bacterial, anti-inflammatory, anti-proliferative, deodorizing, and wound healing activities [1]. Unfortunately, chl a is a hydrophobic molecule, and in water media, it is characterized by low solubility resulting in reduced bioavailability and cellular uptake.…”

Section: Introductionmentioning

confidence: 99%

Stability of Chlorophyll a Monomer Incorporated into Cremophor EL Nano-Micelles under Dark and Moderate Light Conditions

et al. 2020

View full text Add to dashboard Cite

In this paper, stability of chlorophyll a monomers encapsulated into the Cremophor EL nano-micelles was studied under dark and moderate light conditions, typical of a room with natural or artificial lighting, in the presence of oxygen. The pigment stability against visible light was determined using the dynamic light scattering and molecular spectroscopy (UV-Vis absorption and stationary fluorescence) methods. Chlorophyll a, at the molar concentration of 10-5 M, was dissolved in the 5 wt% Cremophor emulsion for comparison in the ethanolic solution. The stability of such a self-assembly pigment–detergent nano-system is important in the light of its application on the commercial-scale. The presented results indicate the high stability of the pigment monomeric molecular organization in the nano-emulsion. During the storage in the dark, the half-lifetime was calculated as about 7 months. Additionally, based on the shape of absorption and fluorescence emission spectra, chlorophyll aggregation in the Cremophor EL aqueous solution along with the time was excluded. Moreover, the average size of detergent micelles as chlorophyll carriers was not affected after 70 days of the nano-system storage. Pigment stability against the moderate white light (0.1 mW) did not differ significantly from storage conditions in the dark. The photooxidation products, detected by occurrence of new absorption and fluorescence emission bands, was estimated on the negligible level. The stability of such a self-assembly pigment–detergent nano-system would potentially broaden the field of chlorophyll a (chl a) application in the food industry, medicine or artificial photosynthesis models.

show abstract

“…Photothermal therapy uses photothermal agents which convert light energy to heat. Eventually, it results in elevated cell temperature, thereby ablating cancer cells effectively without damaging the adjacent normal cells [3][4][5]. Among the various light sources, the light near infra-red (NIR) rang 700-1000 nm is particularly suitable because of low scattering and low absorption by blood and by soft tissue cells in this spectral region [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Today, nanostructures such as nanorods [3], polymers [4], mesoporouse silica nanoparticles [8], nanoshells [9], nanocomposites [8], magnetic nanoparticles [10], nanostars [11] and dendrimers [12,13] are used extensively in medical applications especially cancer imaging and therapy. Gold nanoparticles seem to be an extraordinary candidate for medical applications due to their low toxicity [11,14], stability, easy synthesis and surface modification [11].…”

Section: Introductionmentioning

confidence: 99%

NIR triggered glycosylated gold nanoshell as a photothermal agent on melanoma cancer cells

Nouri

Mohammadi

Mehravi

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Nowadays, gold nanoshells are used in targeted nano photothermal cancer therapy. This study surveyed the application of gold nanoshell (GNs) to thermal ablative therapy for melanoma cancer cells and it takes advantage of the near infrared absorption of gold nanoshells. The synthesis and characterization of glycosylated gold nanoshells (GGNs) were done. The cytotoxicity and photothermal effects of GNs on melanoma cells were evaluated using MTT assay and flow cytometry. The characterization data showed that GGNs are spherical, with a hydrodynamic size of 46.7 nm. Results suggest that the cellular uptake of GGNs was about 78%. Viability assays showed no significant toxicity at low concentrations of GNs. The higher heating rate and toxicity of cancer cells were obtained for the cells exposed to 808 nm NIR laser after incubation with GGNs rather than the GNs. The viability of these cells has dramatically decreased by 29%. Furthermore, 61% more cell lethality was achieved for A375 cells using combined photothermal therapy and treatment with GGNs in comparison to NIR radiation alone. In conclusion, our findings suggest that the synthesized gold/silica core-shell nanoparticles conjugated with glucosamine have high potentials to be considered as an efficient metal-nanoshell in the process of targeted cancer photothermal therapy.

show abstract

Chlorophyll rich biomolecular fraction of A. cadamba loaded into polymeric nanosystem coupled with Photothermal Therapy: A synergistic approach for cancer theranostics

Cited by 48 publications

References 29 publications

Antioxidant and Anticancer Activity of Extract and Fractions Obtained From Diospyros Melanoxylon Roxb. Leaves and Correlation With Their Polyphenolic Profiles

Antioxidant and Anticancer Activity of Extract and Fractions Obtained From Diospyros Melanoxylon Roxb. Leaves and Correlation With Their Polyphenolic Profiles

Stability of Chlorophyll a Monomer Incorporated into Cremophor EL Nano-Micelles under Dark and Moderate Light Conditions

NIR triggered glycosylated gold nanoshell as a photothermal agent on melanoma cancer cells

Contact Info

Product

Resources

About