ChlorophyllaFluorescence Induction in Higher Plants: Modelling and Numerical Simulation

Stirbet, Alexandrina; Govindjee, .; Strasser, Bruno J.; Strasser, Reto J.

doi:10.1006/jtbi.1998.0692

Cited by 169 publications

(86 citation statements)

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“…The dashed line delineates the highlight treatment and recovery periods. reaction centers close or become photoinactivated, the antenna complex may serve adjacent antennae connected to open functional PSII centers, causing a dynamically increasing effective antenna size as reaction centers close (Hecks et al, 1996;Stirbet et al, 1998 4A). In our experimental conditions, the deepwater strain O. sp.…”

Section: Psii Effective Absorption Cross Sectionsmentioning

confidence: 99%

Photosystem II and Pigment Dynamics among Ecotypes of the Green Alga Ostreococcus

et al. 2009

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We investigated the photophysiological responses of three ecotypes of the picophytoplankter Ostreococcus and a larger prasinophyte Pyramimonas obovata to a sudden increase in light irradiance. The deepwater Ostreococcus sp. RCC809 showed very high susceptibility to primary photoinactivation, likely a consequence of high oxidative stress, which may relate to the recently noted plastid terminal oxidase activity in this strain. The three Ostreococcus ecotypes were all capable of deploying modulation of the photosystem II repair cycle in order to cope with the light increase, but the effective clearance of photoinactivated D1 protein appeared to be slower in the deepwater Ostreococcus sp. RCC809, suggesting that this step is rate limiting in the photosystem II repair cycle in this strain. Moreover, the deepwater Ostreococcus accumulated lutein and showed substantial use of the xanthophyll cycle under light stress, demonstrating its high sensitivity to light fluctuations. The sustained component of the nonphotochemical quenching of fluorescence correlated well with the xanthophyll deepoxidation activity. Comparisons with the larger prasinophyte P. obovata suggest that the photophysiology of Ostreococcus ecotypes requires high photosystem II repair rates to counter a high susceptibility to photoinactivation, consistent with low pigment package effects in their minute-sized cells.

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Section: Psii Effective Absorption Cross Sectionsmentioning

confidence: 99%

Photosystem II and Pigment Dynamics among Ecotypes of the Green Alga Ostreococcus

et al. 2009

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“…The complimentary relation between fluorescence and photochemical yield has made fluorescence monitoring a sensitive non-invasive tool for probing the electron transport in PSII (Papageorgiou and Govindjee 2004). Several models have been presented which quantitatively relate the changes in fluorescence yield to the photochemical yield of electron transport to and from PSII (Bouges-Bocquet 1980;Stirbet et al 1998;Vredenberg 2000Vredenberg , 2004Lazár 2003Lazár , 2006Zhu et al 2005).…”

Section: Introductionmentioning

confidence: 99%

On the chlorophyll a fluorescence yield in chloroplasts upon excitation with twin turnover flashes (TTF) and high frequency flash trains

2007

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Chlorophyll fluorescence is routinely taken as a quantifiable measure of the redox state of the primary quinone acceptor Q A of PSII. The variable fluorescence in thylakoids increases in a single turnover flash (STF) from its low dark level F o towards a maximum F m STF when Q A becomes reduced. We found, using twin single turnover flashes (TTFs) that the fluorescence increase induced by the first twin-partner is followed by a 20-30% increase when the second partner is applied within 20-100 ls after the first one. The amplitude of the twin response shows a period-of-four oscillation associated with the 4-step oxidation of water in the Kok cycle (S states) and originates from two different trapped states with a life time of 0.2-0.4 and 2-5 ms, respectively. The oscillation is supplemented with a binary oscillation associated with the two-electron gate mechanism at the PSII acceptor side. The F(t) response in high frequency flash trains (1-4 kHz) shows (i) in the first 3-4 flashes a transient overshoot 20-30% above the F m STF = 3*F o level reached in the 1st flash with a partial decline towards a dip D in the next 2-3 ms, independent of the flash frequency, and (ii) a frequency independent rise to F m = 5*F o in the 3-60 ms time range. The initial overshoot is interpreted to be due to electron trapping in the S 0 fraction with Q B -nonreducing centers and the dip to the subsequent recovery accompanying the reoxidation of the double reduced acceptor pair in these RCs after trapping. The rise after the overshoot is, in agreement with earlier findings, interpreted to indicate a photo-electrochemical control of the chlorophyll fluorescence yield of PSII. It is anticipated that the double exciton and electron trapping property of PSII is advantageous for the plant. It serves to alleviate the depression of electron transport in single reduced Q B -nonreducing RCs, associated with electrochemically coupled proton transport, by an increased electron trapping efficiency in these centers.Keywords Chlorophyll a Á Fluorescence quenching Á Q B -nonreducing center Á Single turnover excitation Á Twin turnover excitation Á Three state trapping mechanism (TSTM)

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“…I vividly remember that once, by error, I called his home phone number instead of the fax and I woke him up in the middle of the night; I felt awful, but he mildly admonished me not to repeat that mistake. This was my first paper with him as co-author, which was published in the Journal of Theoretical Biology in 1998 (Stirbet et al 1998). Then, I met Govindjee in person at the XIth International Photosynthesis Congress in Budapest, Hungary, where I had the privilege to introduce to him some of my Rumanian colleagues from the University of Bucharest, who were extremely impressed.…”

Section: Govindjee As I Know Himmentioning

confidence: 99%

Govindjee at 80: more than 50 years of free energy for photosynthesis

Eaton‐Rye

2013

Photosynth Res

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We provide here a glimpse of Govindjee and his pioneering contributions on the two light reactions and the two pigment systems, particularly on the waterplastoquinone oxido-reductase, Photosystem II. His focus has been on excitation energy transfer; primary photochemistry, and the role of bicarbonate in electron and proton transfer. His major tools have been kinetics and spectroscopy (absorption and fluorescence), and he has provided an understanding of both thermoluminescence and delayed light emission in plants and algae. He pioneered the use of lifetime of fluorescence measurements to study the phenomenon of photoprotection in plants and algae. He, however, is both a generalist and a specialist all at the same time. He communicates very effectively his passion for photosynthesis to the novice as well as professionals. He has been a prolific author, outstanding lecturer and an editor par excellence.

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ChlorophyllaFluorescence Induction in Higher Plants: Modelling and Numerical Simulation

Cited by 169 publications

References 0 publications

Photosystem II and Pigment Dynamics among Ecotypes of the Green Alga Ostreococcus

Photosystem II and Pigment Dynamics among Ecotypes of the Green Alga Ostreococcus

On the chlorophyll a fluorescence yield in chloroplasts upon excitation with twin turnover flashes (TTF) and high frequency flash trains

Govindjee at 80: more than 50 years of free energy for photosynthesis

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