Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker

Cui, Yulin; Qin, Song; Jiang, Peng

doi:10.1371/journal.pone.0098607

Cited by 48 publications

(38 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chloroplast DNA sequences are widely used in genetic engineering [ 24 ] and in reconstructing evolutionary relationships among plants [ 11 , 25 , 26 ]. Complete cp genome sequences harbor enough information to reconstruct both recent and ancient diversifications.…”

Section: Discussionmentioning

confidence: 99%

Complete Chloroplast Genome Sequence of Tartary Buckwheat (Fagopyrum tataricum) and Comparative Analysis with Common Buckwheat (F. esculentum)

et al. 2015

View full text Add to dashboard Cite

We report the chloroplast (cp) genome sequence of tartary buckwheat (Fagopyrum tataricum) obtained by next-generation sequencing technology and compared this with the previously reported common buckwheat (F. esculentum ssp. ancestrale) cp genome. The cp genome of F. tataricum has a total sequence length of 159,272 bp, which is 327 bp shorter than the common buckwheat cp genome. The cp gene content, order, and orientation are similar to those of common buckwheat, but with some structural variation at tandem and palindromic repeat frequencies and junction areas. A total of seven InDels (around 100 bp) were found within the intergenic sequences and the ycf1 gene. Copy number variation of the 21-bp tandem repeat varied in F. tataricum (four repeats) and F. esculentum (one repeat), and the InDel of the ycf1 gene was 63 bp long. Nucleotide and amino acid have highly conserved coding sequence with about 98% homology and four genes—rpoC2, ycf3, accD, and clpP—have high synonymous (Ks) value. PCR based InDel markers were applied to diverse genetic resources of F. tataricum and F. esculentum, and the amplicon size was identical to that expected in silico. Therefore, these InDel markers are informative biomarkers to practically distinguish raw or processed buckwheat products derived from F. tataricum and F. esculentum.

show abstract

Section: Discussionmentioning

confidence: 99%

Complete Chloroplast Genome Sequence of Tartary Buckwheat (Fagopyrum tataricum) and Comparative Analysis with Common Buckwheat (F. esculentum)

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The bar gene was also described as a selection marker in tobacco (Lutz et al, 2001 ). The use of this system in algae resulted in the development of a useful tool and a suitable selection system for this algae that is not sensitive to most commonly used antibiotics, such as spectinomycin, streptomycin or kanamycin (Cui et al, 2014 ).…”

Section: Chloroplast Transformation Systemsmentioning

confidence: 99%

Transgene Expression in Microalgae—From Tools to Applications

2016

View full text Add to dashboard Cite

Microalgae comprise a biodiverse group of photosynthetic organisms that reside in water sources and sediments. The green microalgae Chlamydomonas reinhardtii was adopted as a useful model organism for studying various physiological systems. Its ability to grow under both photosynthetic and heterotrophic conditions allows efficient growth of non-photosynthetic mutants, making Chlamydomonas a useful genetic tool to study photosynthesis. In addition, this green alga can grow as haploid or diploid cells, similar to yeast, providing a powerful genetic system. As a result, easy and efficient transformation systems have been developed for Chlamydomonas, targeting both the chloroplast and nuclear genomes. Since microalgae comprise a rich repertoire of species that offer variable advantages for biotech and biomed industries, gene transfer technologies were further developed for many microalgae to allow for the expression of foreign proteins of interest. Expressing foreign genes in the chloroplast enables the targeting of foreign DNA to specific sites by homologous recombination. Chloroplast transformation also allows for the introduction of genes encoding several enzymes from a complex pathway, possibly as an operon. Expressing foreign proteins in the chloroplast can also be achieved by introducing the target gene into the nuclear genome, with the protein product bearing a targeting signal that directs import of the transgene-product into the chloroplast, like other endogenous chloroplast proteins. Integration of foreign genes into the nuclear genome is mostly random, resulting in large variability between different clones, such that extensive screening is required. The use of different selection modalities is also described, with special emphasis on the use of herbicides and metabolic markers which are considered to be friendly to the environment, as compared to drug-resistance genes that are commonly used. Finally, despite the development of a wide range of transformation tools and approaches, expression of foreign genes in microalgae suffers from low efficiency. Thus, novel tools have appeared in recent years to deal with this problem. Finally, while C. reinhardtii was traditionally used as a model organism for the development of transformation systems and their subsequent improvement, similar technologies can be adapted for other microalgae that may have higher biotechnological value.

show abstract

“…Platymonas subcordiformis bar Phosphinothricin acetyltransferase [63] Porphyridium spp. AHAS Acetohydroxyacid synthase [71] Nannochloropsis oceanica gfp, BLE Green florescent protein, Zeocin resistance [66] Euglena gracils aadA Resistance to spectionomycin and streptomycin [72] Haematococcus pluvialis aadA Resistance to spectionomycin and streptomycin [73] Dunaliella tertiolecta Xylanase, α-galactosidase, phytase, phosphate anhydrolase, and β-mannanase…”

Section: Algal Species Gene Of Interest Trait Referencementioning

confidence: 99%

Engineering microalgae through chloroplast transformation to produce high‐value industrial products

Siddiqui

Wei

Boehm

et al. 2019

Biotech and App Biochem

View full text Add to dashboard Cite

The last few years have seen an ever‐increasing interest in the exploitation of microalgae as an alternative platform to produce high‐value products such as biofuels, industrial enzymes, therapeutic proteins, including antibodies, hormones, and vaccines. Due to some unique attractive features, engineering of the chloroplast genome provides a promising platform for the production of high‐value targets because it allows manipulation of metabolic processes in ways that would be impossible, or at least prohibitively difficult through traditional approaches. Since its initial demonstration in 1988 in Chlamydomonas reinhardtii, genetic tools have been developed, which have made it possible to produce high‐value molecules in different species. However, the commercial application of microalgae as production platform is hindered by many factors like poor biomass, low product yields, and costly downstream processing methodologies. In this review, we discuss the potential of microalgae to use as an alternative production platform for high‐value targets using chloroplast transformation technology.

show abstract

Chloroplast Transformation of Platymonas (Tetraselmis) subcordiformis with the bar Gene as Selectable Marker

Cited by 48 publications

References 36 publications

Complete Chloroplast Genome Sequence of Tartary Buckwheat (Fagopyrum tataricum) and Comparative Analysis with Common Buckwheat (F. esculentum)

Complete Chloroplast Genome Sequence of Tartary Buckwheat (Fagopyrum tataricum) and Comparative Analysis with Common Buckwheat (F. esculentum)

Transgene Expression in Microalgae—From Tools to Applications

Engineering microalgae through chloroplast transformation to produce high‐value industrial products

Contact Info

Product

Resources

About