Chloroquine and Vitamin Combination Effects on P. berghei Induced Oxidative Stress

Abstract

Aim: The effect of chloroquine, folic and ascorbic acid on malaria parasite induced oxidative stress was the focus of this study. The study relevance derives from the need to understand the specific roles of these individual organic acids used in combination with chloroquine. Study Design: The design involves five groups of control (non-parasitized-nontreated), parasitized nontreated (PnT), parasitized chloroquine and ascorbic acid treated (Pcq+asT), parasitized chloroquine and folic acid treated (Pcq+faT) and parasitized chloroquine, ascorbic and folic acid treated (Pcq+asT+faT). Place and Duration of Study: Department of Biochemistry Ambrose Alli University (Faculty of Natural Sciences). This study is part of a research that lasted three years. Materials and Methods: Treatment regime was for three days after parasitemia in mice was established with Gemsa stain. All biochemical and haematological parameters assayed for in this project were conducted using standard procedures. Result: Chloroquine and vitamin treatments significantly (P=.05) reduced erythrocyte fragility (EF), total bilirubin and increased packed cell volume (PCV) when compared with PnT parameters of mice. Treatments significantly (P=.05) increased serum albumin compared with control and had no effect on the serum albumin levels of PnT mice. Treatment with cq+asa and cq+as+fa resulted in significant (P=.

Biochemical and Histopathological Effects of Coadministration of Amodiaquine, Artesunate, and Selenium on Plasmodium Berghei Infected Mice

“…The distortion of the hepatocyte membrane which has been linked to acute liver damage results in the leakage of cytosolic contents of hepatocytes from its membrane [22]. Our observation is in correlation with the findings of Iyawe and Onigbinde [23] as the liver parameters; AST and ALT were not altered significantly with treatment. ALP activity was significantly higher (p<0.05) in AMO treated mice when compared with the normal control group which may indicate biliary obstruction.…”

Oxidative Stress in Malaria: Potential Benefits of Antioxidant Therapy

“…However, in a study conducted by Iyawe and Onigbinde [ 225 ], who sought to identify the effect of treatment with chloroquine, folic acid, and ascorbic acid on malaria, it was observed that the combined treatment between chloroquine and folic acid was more effective than ascorbic acid combined with chloroquine in the experimental malaria model in mice. In a recent study, Ebohon et al [ 226 ] identified that the co-administration of vitamin C with oral artesunate-amodiaquine in experimental malaria may be beneficial because it reduced oxidative stress and increased the gene expression of antioxidant enzymes in mice infected by Plasmodium berghei .…”