Chloroquine stripping of HLA A,B antigens from red cells

Swanson, Jane L.; Sastamoinen, Robert

doi:10.1046/j.1537-2995.1985.25586020120.x

Cited by 16 publications

(11 citation statements)

References 5 publications

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“…We thought that Bg antibodies could be identified by the following examinations: 1) detection of the HLA antibody with the specificity of corresponding anti‐Bg in the patient's serum; 2) losing the reactivity by destruction of Bg antigens (HLA Class I antigens) by chloroquine diphosphate 8 or solution of glycine‐HCl–ethylenediaminetetraacetate; 15 and 3) absorption of HLA antibodies in patient's serum with pooled PLTs.…”

Section: Discussionmentioning

confidence: 99%

“…Antibody was eluted from the patient's RBCs with dichloromethane dichloropropan (DT‐reagent II, Ortho Clinical Diagnostics). To denature HLA Class I antigens present on RBCs, chloroquine treatment 8 was used. The patient's serum sample was treated with 0.01 mol per L dithiothreitol (DTT; Wako Pure Chemical Industries, Osaka, Japan) at 37°C for 15 minutes to distinguish IgG class antibody from IgM antibody 9 …”

Section: Methodsmentioning

confidence: 99%

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Delayed and acute hemolytic transfusion reactions resulting from red cell antibodies and red cell–reactive HLA antibodies

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Delayed and acute hemolytic transfusion reactions resulting from red cell antibodies and red cell–reactive HLA antibodies

et al. 2005

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“…For removal of Bg antigens, Swanson and Sastamoinen 13 and Champagne et al 14 treated RBCs with commercially available chloroquine (Gamma-Quin; now available from Immucor, Norcross, GA) at room temperature for 90 minutes 13 or up to 2 hours. 14 Indications CDP treatment can be used when a patient's RBCs are coated with IgG and there is a need to perform antigen typing using antisera that are reactive only at the IAT phase and/or to prove the presence of an autoantibody by testing a patient's plasma, serum, or eluate against DAT-autologous RBCs.…”

Section: Procedural Stepsmentioning

confidence: 99%

Utility of chloroquine diphosphate in the blood bank laboratory

Aye

Arndt

2018

Immunohematology

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Chloroquine diphosphate (CDP) is a helpful tool in the blood bank for two main applications. The most common application is to render direct antiglobulin test-positive red blood cells (RBCs) free from membrane-bound IgG; these treated RBCs can then be used for autologous adsorption and/or to determine the patient's RBC phenotype. Another common use of CDP is to remove human leukocyte antigens (HLAs) from RBCs to help identify or exclude the presence of antibodies to HLAs expressed on RBCs, for example, Bennett-Goodspeed (Bg) antigens. In this review, the principles, applications, and limitations of using CDP are discussed. Immunohematology 2018;34:98-102.

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“…Both chloroquine diphosphate and EDTA glycine acid treatments destroy Bg antigens . When the reactivity on known or suspected Bg+ cells is abolished following treatment of the test cell with either reagent, the sample likely contains Bg antibodies.…”

Section: Removal Of Igg From Autologous Cellsmentioning

confidence: 99%

Powerful tools for resolving antibody problems

Hamilton

2016

ISBT Science Series

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Background Identifying the specificity of antibodies in a patient's plasma can be as straightforward as testing selected reagent red cells on the basis of the antibody detection results. Many investigations, however, require the use of additional methods or supplemental tests for resolution. Aims Specifics related to performance of these procedures, their interpretations and their limitations were reviewed. Discussion Gel microcolumn or solid phase methods, polyethylene glycol or low‐ionic strength saline enhancement media or unenhanced tests have appropriate uses for detecting or avoiding antibody reactivity. Useful red cell treatments include enzymes or thiol compounds to destroy antigens or enhance the antigen–antibody interaction and EDTA–glycine acid or chloroquine diphosphate to remove bound IgG from autologous cells. Separating autologous red cells from transfused donor cells for phenotyping or testing with plasma or eluate is critical. Alloantibodies or autoantibodies can be removed or separated by appropriate adsorption tests. Inhibition of antibody reactivity by a known soluble blood group substance confirms specificity or neutralizes interfering reactivity. Conclusion Combined into a logical investigation, these additional tests will aid in resolution of most antibody‐containing samples.

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Chloroquine stripping of HLA A,B antigens from red cells

Cited by 16 publications

References 5 publications

Delayed and acute hemolytic transfusion reactions resulting from red cell antibodies and red cell–reactive HLA antibodies

Delayed and acute hemolytic transfusion reactions resulting from red cell antibodies and red cell–reactive HLA antibodies

Utility of chloroquine diphosphate in the blood bank laboratory

Powerful tools for resolving antibody problems

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