“…Western blotting is a widely-used technique for evaluating relative expression levels of polysialylated-proteins in biological samples (von Der Ohe et al, 2002;Korja et al, 2009;Valentiner, Muhlenhoff, Lehmann, Hildebrandt, & Schumacher, 2011;Nishimura et al, 2014;Westphal, Kleene, Lutz, Theis, & Schachner, 2016;Abe et al, 2017;Chen, Ren, Zhang, Troy, & Wang, 2017;Klobucar et al, 2017). Similarly to ELISA, Western blotting also recognises polySia proteins via immunodetection.…”