Choice of Extraction Procedure for Estimation of Anterior Pituitary Hormone Content.

Ishikawa, Junko; Fuse, Yusuke; Wakabayashi, Keiji

doi:10.1507/endocrj1954.34.755

Cited by 22 publications

(12 citation statements)

References 18 publications

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“…Hence high expression of PRL in a small number of individuals would be diluted by the RNA from the other individuals in the total sample. The absence of POMC may reflect a low content of ACTH in the anterior pituitary gland (Matsuyama et al 1971, Moldow & Yalow 1978, Ishikawa et al 1987. In agreement with our profile, previously measured expression levels for FSH beta and LH beta showed similar quantitative mRNA measurements (Dalkin et al 1989).…”

Section: Gene Expression Profile Of the Human Pituitary Glandsupporting

confidence: 90%

Expression profile of active genes in the human pituitary gland

Tanaka

Tatsumi

Okubo³

et al. 2002

Journal of Molecular Endocrinology

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To characterize transcripts abundantly expressed in the human pituitary gland in general as well as to isolate novel transcripts expressed specifically in this gland, we generated an expression profile of the active genes transcribed in it. A total of 1015 randomly collected 3′ expressed sequence tags (ESTs) (gene signatures, GSs) were grouped into 527 GS species. The results showed the relative expression levels of genes in the pituitary gland. The genes comprising more than 1% of total mRNA were prolactin, growth hormone and chromogranin B genes. When known genes were categorized, the genes for pituitary hormones were the most actively transcribed, followed by the genes for ribosomal proteins, nuclear proteins and secretory granule proteins. Through comparison of this gene expression profile with the BodyMap database containing profiles generated from 63 other human tissues, we obtained 11 genes which appeared to be specifically expressed in the pituitary gland. In addition to the eight known genes, we identified three novel pituitary-specific transcripts which encode putative proteins: pituitary gland specific factor 1a (PGSF1a), PGSF1b and PGSF2. This expression profile method is a novel approach to the isolation of pituitary-specific genes that may have important functions.

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Section: Gene Expression Profile Of the Human Pituitary Glandsupporting

confidence: 90%

Expression profile of active genes in the human pituitary gland

Tanaka

Tatsumi

Okubo³

et al. 2002

Journal of Molecular Endocrinology

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“…A plasma sample was obtained by centrifugation (3000 rpmϫ15 min). ACTH 15) and gonadotropin 16,17) were determined by radioimmunoassay. Catecholamine 18) was determined by HPLC (SRL, Hino, Tokyo).…”

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confidence: 99%

Effects of Inhaling the Vapor of Lavandula burnatii super-Derived Essential Oil and Linalool on Plasma Adrenocorticotropic Hormone (ACTH), Catecholamine and Gonadotropin Levels in Experimental Menopausal Female Rats

Yamada

Mimaki

Sashida

2005

Biological & Pharmaceutical Bulletin

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Lavender is one of the most common of the various herbs known to have sedative and hypnotic effects on rodents and humans, and it has been reported to have anti-neurodepressive effects. 1,2) Recently, inhalation of the vapor of many herbal oils has been used for aromatherapy. [3][4][5] We previously reported that pentetrazol or nicotine-induced convulsion in mice was inhibited by the inhalation of vapors of Lavandula angustifolia oil. 6) In this study, we selected Lavandula burnatii super-derived essential oil is from the five main lavender oils. It has a good fragrance and lower cost than other lavender oils, but contains in a high level of linalool. 7)Elisabetsky et al., already reported that linalool showed hypothermic, hypnotic and anti-convulsive effects in mice. 8) In women, exclusively, the function of the ovaries decreases with aging, causing a hormone imbalance based on increasing gonadotropine and decreasing estrogen. 9) Ether-inhalation is one experimental animal model for stress. 10) After inhalation, an increase in adrenocorticotropic hormone (ACTH) and decrease in catecholamine level in plasma is induced. These observations are based on accerelation of the turnover of catecholamine in the central nervous system. 11)These phenomena, namely plasma ACTH and catecholamine levels, follow the same movement in human depression. 12)Accordingly, climacterium syndrome shows many symptoms including autonomic nervous system disorder or depression.9) In this study, we report the assessment of the effects of lavandula burnetii super-derived essential oil and linalool on plasma ACTH, catecholamine and gonadotropin (lutinizing hormone: LH, folicle-stimulating hormone: FSH) levels in an experimental menopausal model using female rats under ether-inhalation. MATERIALS AND METHODWistar female retired rats that had delivered 3 or 4 times were obtained from Nihon Ikagaku Laboratory (Tokyo), and ovariectomy (OVX) was carried out under Nembutal anesthesia. These rats were housed at a constant temperature (23Ϯ1°C) and a constant relative humidity (55Ϯ5%), with a constant light cycle (lights on from 6:30 a.m. to 6:30 p.m.). Food and water were available ad libitum. One month after OVX, the rats were used for our experiment. Lavender oils were purchased from Kenso Igakusha (Tokyo) and linalool was obtained from Wako Pure Chemical Ind., Ltd. (Tokyo). The inhalation apparatus was a plastic cage (H13ϫW21ϫ L32 cm with a cover). Cotton was soaked in two doses (0.3 or 0.7 ml) of lavender oil or linalool and placed in a plastic cage. The temperature in the experiment room was 23Ϯ1°C. Inhalation of lavender oil and linalool vapor was performed twice (at 1100, 1500 h) a day for three days. One trial involved 20 min inhalation according to the method of Jirovetz et al. 13) After a 10 min final inhalation on the 3rd day, rats inhaled ether (cotton soaked 3 ml) vapor for 20 min. The procedure of ether inhalation was carried out according to the method of Ina et al. 14) After 20 min, rats were decapitated and blood samples were obtained...

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“…Pituitary protein extracts were prepared as described by Ishikawa et al [7]. A protein assay kit (Bio-Rad Laboratories, Richmond, CA, USA) was employed to measure the protein concentration of pituitary extracts using bovine serum albumin as a standard, followed by dilution at 10 mg/ml.…”

Section: Preparation Of Pituitary Extractsmentioning

confidence: 99%

Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone.

Tomizawa

Ogawa

Katō

et al. 2002

Journal of Reproduction and Development

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Abstract. This study aimed to develop an antiserum specific to follicle stimulating hormone (FSH), for a wide range of species, free from cross-reactivity against luteinizing hormone. An antiserum against recombinant porcine FSH (rec-pFSH) expressed in insect cells was raised in a rabbit and its specificity was characterized by time-resolved fluoroimmunoassay (TR-FIA) and immunohistochemistry. The titer of the anti-rec-pFSH antiserum was about 1:5,000 for use in TR-FIA. The inhibition curve of TR-FIA with rec-pFSH paralleled that of pituitary-derived FSH, indicating that the antigenicities of the two FSH preparations do not differ significantly. The anti-rec-pFSH antiserum showed low reactivities with FSHs prepared from sheep and rat, suggesting it was species-and hormone-specific, contrary to our intention. The TR-FIA showed that this antiserum did not react with pituitary extracts from mammals other than the pig. However, immunohistochemistry demonstrated that this antiserum reacted with the specific cells not only of the porcine anterior pituitary but also of the cow, goat, and horse. The antiserum also immunostained pituitary cells of the cow, goat and horse. Therefore, this anti-rec-pFSH antiserum will be of limited use in studies involving competitive immunoreactions due to its species-specific reactivity, but it should be useful for non-competitive techniques, such as immunohistochemistry.

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Choice of Extraction Procedure for Estimation of Anterior Pituitary Hormone Content.

Cited by 22 publications

References 18 publications

Expression profile of active genes in the human pituitary gland

Expression profile of active genes in the human pituitary gland

Effects of Inhaling the Vapor of Lavandula burnatii super-Derived Essential Oil and Linalool on Plasma Adrenocorticotropic Hormone (ACTH), Catecholamine and Gonadotropin Levels in Experimental Menopausal Female Rats

Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone.

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