Cholecystokinin (CCK) and its receptors (CCK1R and CCK2R) in chickens: functional analysis and tissue expression

Wan, Ying; Deng, Qiuyang; Zhou, Zhichun; Deng, Yulin; Zhang, Jiannan; Li, Juan; Wang, Yajun

doi:10.1016/j.psj.2022.102273

Cited by 12 publications

(14 citation statements)

References 79 publications

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“…Similar innervation was observed in ancient jawless fish such as lamprey [27] and modern teleosts such as the goldfish [47]. Modern oviparous tetrapods were also shown to express the CCK receptor in their pituitary glands [48],…”

Section: Discussionsupporting

confidence: 56%

The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion

Cohen

Shulman

Fontanaud

et al. 2023

Preprint

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Many fish species display clear temporal separation between prolonged periods of energetically costly gonadal development, and short spawning events. These two distinct processes are controlled by the gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH), respectively. While it was suggested that a common secretagogue, gonadotropin-releasing hormone (GnRH), controls both functions, it is unclear how the secretion of the two gonadotropins is differentially regulated. Here, we usedin vivoandex vivocalcium imaging of zebrafish gonadotrophs to study the regulation of gonadotropin release in fish. We show that while LH cells are highly responsive to GnRH stimulation, the response of FSH cells is weak and inconsistent. By combining transcriptomics and calcium imaging, we found that FSH cells express the receptor for the satiety hormone cholecystokinin (CCK) and display a strong calcium response to its application, accompanied by FSH secretion, which designates this peptide as abona fideFSH secretagogue. From an evolutionary perspective, these findings indicate that the control of folliculogenesis and ovulation in fish was placed under different neural circuits, which respond to the specific somatic and environmental cues relevant to each process.

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Section: Discussionsupporting

confidence: 56%

The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion

Cohen

Shulman

Fontanaud

et al. 2023

Preprint

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“…CCK activity is mediated by its receptors (Dufresne et al, 2006). After ligand activation, CCK1R and CCK2R stimulate calcium mobilization and the MAPK/ERK and cAMP/PKA signalling pathways (Wan et al, 2023; Zeng et al, 2020). Thus, the expression of CCK1R and CCK2R reinforces the fact that oviductal CCK could regulate sperm capacitation after binding to them, modulating the uptake of HCO 3 − , also in porcine species.…”

Section: Discussionmentioning

confidence: 99%

“…CCK peptides located in the sperm acrosome have been suggested to be released during the acrosome reaction, which might facilitate sperm motility during egg penetration (Pelto‐Huikko et al, 1989; Persson et al, 1988, 1989; Sasse et al, 2000). CCK and its receptors were also detected in chicken's ovary and uterus (Wan et al, 2023), and CCK receptors on frog oocytes (Moriarty et al, 1988), although, as far as we are concerned, no receptors have been described in mammalian oocytes or mammalian female reproductive tissues.…”

Section: Introductionmentioning

confidence: 94%

“…CCK1R and CCK2R stimulate calcium mobilization and the MAPK/ ERK and cAMP/PKA signalling pathways(Wan et al, 2023;Zeng et al, 2020). Thus, the expression of CCK1R and CCK2R reinforcesF I G U R E 2 Bar chart showing the effect of CCK (Control-0 vs. CCK-25 vs. CCK-50) in sperm function (1-day or 5-day seminal doses) after incubation in capacitation conditions (TALP-25 mmol/L HCO 3 − ).…”

mentioning

confidence: 92%

“…Its presence in the OF in the postovulatory stage could indicate a role in sperm functionality. Thus, CCK expression was found in rat epididymis (Persson et al, 1989) and guinea pig, monkey, mouse, pig, rat, and chicken testis (Persson et al, 1988; Persson et al, 1989; Wan et al, 2023). On the other hand, CCK‐like peptides were detected in spermatocytes and spermatids of monkey, mouse, rat, and human species (Pelto‐Huikko et al, 1989; Persson et al, 1989; Schalling et al, 1990); specifically in the sperm acrosome of monkey and human (Pelto‐Huikko et al, 1989; Persson et al, 1989; Schalling et al, 1990); and CCK and its receptors (CCK1R and CCK2R) have also been detected in the acrosome region of mature mouse sperm (Zhou et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Boar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions

Moros‐Nicolás,

López‐Úbeda,

Luongo

et al. 2023

Reprod Domestic Animals

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In a previous study, our group detected the cholecystokinin (CCK) protein in the porcine oviduct. This fact, together with the involvement of CCK in the regulation of sperm protein tyrosine phosphorylation by the modulation of HCO3− uptake (in mice and humans) suggests a role for CCK during sperm capacitation. Therefore, on the one hand, the expression of CCK receptors (CCK1R and CCK2R) on boar testes has been investigated and probed; on the other hand, boar spermatozoa (from seminal doses of 1‐day and 5‐day storage) were exposed to different concentrations of CCK (0‐control, 25 or 50 μM) in a medium supporting capacitation supplemented with 0, 5 or 25 mmol/L of HCO3− for 1 h at 38.5°C. Sperm motion (total and progressive motility), kinetic parameters, viability, acrosome status, and mitochondrial activity were determined. No differences between groups (0, 25 or 50 μM of CCK) were observed when HCO3− was absent in the media (p > .05). However, the results showed that when the media was supplemented with 5 mmol/L HCO3− in 1‐day seminal dose storage, the linearity index (LIN, %), straightness index (STR, %) and oscillation index (WOB, %) (sperm kinetics parameters) increased in the presence of CCK regardless the concentration (p < .05). Nevertheless, CCK in sperm from 5‐day storage only increased the WOB parameter in comparison to the control (p < .05). Furthermore, the average amplitude of the lateral displacement of the sperm head (ALH, μm) and curvilinear velocity (VCL, μm/s) decreased when CCK was present, depending on its concentration and sperm aging (1‐day vs. 5‐days) (p < .05). In the case of the media supporting capacitation supplemented with 25 mmol/L HCO3−, any differences were observed except for sperm viability in the 5‐day seminal doses, which increased in the 50 μM‐CCK group compared to the control (p < .05). In conclusion, these data suggest an implication of CCK protein during sperm capacitation under low bicarbonate concentration increasing the sperm linear trajectory.

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Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation

Wan,

Zhang,

Zhang

et al. 2025

Poultry Science

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Cholecystokinin (CCK) and its receptors (CCK1R and CCK2R) in chickens: functional analysis and tissue expression

Cited by 12 publications

References 79 publications

The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion

The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion

Boar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions

Characterization of the C5H11ORF96 gene in chickens: cloning, tissue distribution and investigation of its potential function in stress response regulation

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