Cholecystokinin-coupled intracellular signaling in human gallbladder muscle

Yu, Peirong; Petris, Giovanni De; Biancani, Piero; Amaral, Joseph F.; Behar, José

doi:10.1016/0016-5085(94)90713-7

Cited by 46 publications

(38 citation statements)

References 35 publications

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“…The partially digested muscle layer was rinsed with "cytosolic buffer" (4,42,(45)(46)(47). After cells were dispersed, the cell suspension was briefly treated with saponin (75 g/ml) during centrifugation at 200 g for 3 min.…”

Section: Methodsmentioning

confidence: 99%

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Reactive oxygen species (H₂O₂): effects on the gallbladder muscle of guinea pigs

Xiao

Andrada²,

Biancani

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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The level of lipid peroxidation as well as the PGE2 content was significantly increased after H2O2 pretreatment. Unlike superoxide dismutase, the free radical scavenger catalase prevented the H2O2 induced contraction, and its inhibition of the CCK-8 induced contraction. It is concluded that ROS cause damage to the plasma membrane of the gallbladder muscle and contraction through the generation of PGE2 induced by cPLA2-cyclooxygenase and probably mediated by the PKC-MAPK pathway. lipid peroxidation; prostaglandin E2 content; smooth muscle REACTIVE OXYGEN SPECIES (ROS) such as superoxide radical anion, hypochlorous acid, and hydrogen peroxide (H 2 O 2 ) are by-products of oxidative metabolism, in which energy activation and electron reduction are involved. Inflammatory processes enhance their production, which could affect cell functions (20). The mechanisms whereby ROS act at the cellular level are not fully understood. Their pathogenic role on cell membranes and signal-transduction pathways may explain the early events that are associated with cell dysfunction caused by inflammation. Therefore, the understanding of these mechanisms is important for developing therapeutic strategies at cellular sites of dysfunction.Acute cholecystitis is characterized by an inflammatory process that involves the gallbladder wall with polymorphonuclear cells (23). ROS play a major role in mediating cell injury during inflammatory processes. Disorders of gallbladder motility caused or aggravated by inflammatory processes may be due to the actions of ROS. They may also contribute to the inhibition of agonist-induced contraction (25, 37). The actions of ROS are mediated in part by cyclooxygenase by-products that are important participants in the inflammatory process (16). The contraction of tracheal and ileal muscle strips induced by ROS in vitro appear to be mediated by prostaglandins (24,29).Prostaglandins have varied effects on gastrointestinal motility (5,12,17,32). Muscle strips from various segments of guinea pig stomach exhibited different responses to PGE 1 , PGE 2 , and PGF 2␣ (22). In rat stomach, PGE 2 caused a contraction in the longitudinal preparation but had no effect on the circular muscle (35). PGE 1 , PGE 2 , and PGF 2␣ potentiated the contractile responses of guinea pig gallbladder to transmural stimulation and ACh (28). Prostaglandins may also be involved in muscle cytoprotection since PGE 2 and other prostaglandins appear to participate in the pathway responsible for increased formation of scavengers of free radicals (2). These studies therefore were aimed at examining the effects of ROS on gallbladder muscle function utilizing H 2 O 2 on enzymatic digested single muscle cells from normal guinea pig gallbladders. MATERIALS AND METHODSAnimals. Adult male guinea pigs were purchased from Elm Hill Breeding Laboratory (Chelmsford, MA). The Animal Welfare Committee of Rhode Island Hospital approved their use. Animals were housed in thermoregulated rooms and had free access to food and water. After an overnight fast, th...

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Section: Methodsmentioning

confidence: 99%

“…Single muscle cells were obtained by enzymatic digestion (42,(45)(46)(47). Gallbladder muscle layer was cut into 2-mm-wide strips and digested in HEPES buffer containing 0.5 mg/ml type F collagenase and 2 mg/ml papain (activity of ϳ13.9 U/mg protein) for 20 min at 35°C in a shaking water bath.…”

Section: Methodsmentioning

confidence: 99%

Reactive oxygen species (H₂O₂): effects on the gallbladder muscle of guinea pigs

Xiao

Andrada²,

Biancani

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…5 show that BSA is a critical determinant of the ability of physiological concentrations of CCK-8 (1-10 pM; ref. 27) to cause contraction of gastrointestinal smooth muscle and can account for the difference in sensitivity to CCK-8 between dispersed muscle cells and strips of muscle in vitro (14,15,(23)(24)(25)(26). CCK-8 binding to BSA makes it likely, at least for gastrointestinal smooth muscle cells, that the BSA-CCK-8 complex has much greater biologic activity than the free, uncomplexed peptide.…”

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confidence: 99%

“…Previous studies have shown that CCK-8 causes contraction of dispersed gastrointestinal smooth muscle cells in vitro at concentrations that are 100 to 1000 times lower than those that cause contraction of strips of smooth muscle mounted in a chamber (14,15,(23)(24)(25)(26). BSA is usually included in the incubation solution used to study dispersed smooth muscle cells in vitro, but it is usually not included in the incubation solution used to study strips of gastrointestinal smooth muscle because BSA causes too much foaming when oxygen is bubbled through the chamber.…”

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confidence: 99%

Modulation of cholecystokinin activity by albumin.

Huang¹,

Talkad²,

Fortune³

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

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“…Postprandial gallbladder emptying is triggered mainly by plasma CCK from small intestine. CCK interacts with CCK receptor-1 (CCK-R) in gallbladder smooth muscle cells, which in turn elicits the contraction of gallbladder by the activation of post-membrane signaling passway [20]. We hypothesize that abnormal gallbladder contraction in response to CCK, may play an important role in the development of cholesterol gallstone in hyperglycemic patient.…”

Section: Introductionmentioning

confidence: 99%

Reduced bile duct contractile function in rats with chronic hyperglycemia

Liu¹,

Su²,

Wang³

et al. 2010

Health

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The incidence of gallstone is higher in patients with diabetes mellitus than in general population. It is generally attributed to hypomotility and lowered emptying function of the gallbladder. In this study, we investigate if chronic hyperglycemia is correlated with reduced contractile function of the bile ducts in rat. Hyperglycemic rats were induced by streptozotocin-nicotinamide treatment. Hyperglycemic rats were sacrificed eight months after induction and bile ducts were removed for the subsequent studies. The bile duct contractility of the normal rats is consistently higher than that of the hyperglycemic rats. The contractities were measured to be 5.5 ± 0.2 mg vs. 4.2 ± 0.1 mg without CCK stimulation, and 5.5 ± 0.3 mg vs. 7.9 ± 0.4 mg with CCK stimulation, respectively for hyperglycemic and normal rats. There was no significant difference in plasma CCK concentration in hyperglycemic rats and normal rats. The expression of CCK-A receptor protein in the bile duct tissue was decreased in hyperglycemic rats compared with that of the normal rats, and it may, at least in part, responsible for a reduced contractility. A reduced bile duct motility may cause bile retention, and may be one of the factors predispose to gallstone formation in type 2 diabetes patients, which is characterized with chronic hyperglycemia.

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Cholecystokinin-coupled intracellular signaling in human gallbladder muscle

Cited by 46 publications

References 35 publications

Reactive oxygen species (H₂O₂): effects on the gallbladder muscle of guinea pigs

Reactive oxygen species (H₂O₂): effects on the gallbladder muscle of guinea pigs

Modulation of cholecystokinin activity by albumin.

Reduced bile duct contractile function in rats with chronic hyperglycemia

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Cholecystokinin-coupled intracellular signaling in human gallbladder muscle

Cited by 46 publications

References 35 publications

Reactive oxygen species (H2O2): effects on the gallbladder muscle of guinea pigs

Reactive oxygen species (H2O2): effects on the gallbladder muscle of guinea pigs

Modulation of cholecystokinin activity by albumin.

Reduced bile duct contractile function in rats with chronic hyperglycemia

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Product

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Reactive oxygen species (H₂O₂): effects on the gallbladder muscle of guinea pigs

Reactive oxygen species (H₂O₂): effects on the gallbladder muscle of guinea pigs