Cholesterol Assimilation of Saccharomyces cerevisiae B-18 isolated from gastrointestinal tract of Javanese duck

Abstract

This study had a purpose of obtaining potential indigenous yeasts for assimilating cholesterol and assessed the in vitro activity of Bile Salt Hydrolase (BSH) using Chloramphenicol Yeast Glucose (CYG) media supplemented CaCl2 and Taurodeoxycholic Acid (TDCA). Yeasts were collected from the gastrointestinal tract of Indonesian native chicken (Gallus javanicus), Javanese duck (Anas javanicus), and Muscovy duck (Anas moschata). The BSH assay was performed to determine secretion of BSH from yeast strain to conjugate bile salts into cholic acid-free by measuring precipitation zone in a specific medium. The quantitative measurement to assimilate cholesterol in yeast using CYG broth contained soluble cholesterol (500 ppm) and incubated at 30°C for 72 hours. Microplate reader used to analyze cholesterol content in the supernatant. Data were analyzed using Analysis of Variance (ANOVA) with one way completely randomized. Precipitation zone found among isolates did not significantly different (P>0.05). Isolate B-18 from Javanese duck performed the highest percentage of assimilating cholesterol with the value of 51.83% and identified as S. cerevisiae. This isolate was closely related to S. cerevisiae mt 21s (accession number X00149.1)based on phylogenetic tree analysis. It could be concluded that S. cerevisiae B-18was potential for assimilating cholesterol in vitro.

The Assays of Bacteria-Yeast Consortia as Probiotics Candidates and Their Influences on Nutrients Utilization of Quails Diet

“…It would be possible to deconjugate bile salt and might be effective in reducing serum cholesterol in poultry. In the previous study, isolates Lp AKK-30 and Sc B-8 showed lowering cholesterol activities (Julendra et al, 2017;Istiqomah et al, 2018).…”

“…In the previous study, S. cerevisiae has a potency to reduce cholesterol accumulation in the animal product (Istiqomah et al, 2018). Yeast positively interacts with bacteria by producing amino acids and vitamins for stimulating bacterial growth (Hatoum et al, 2012).…”

“…Isolate and sample preparation. Isolates used in this experiment consisted of Lactobacillus plantarum AKK-30 (isolated from native chicken) and Saccharomyches cerevisiae B-18 (isolated from Javanese duck) were molecularly identified by Julendra et al (2017) and Istiqomah et al (2018), respectively. Pathogenic bacteria used in the in vitro assay were Salmonella pullorum ATCC 13036, Pseudomonas aeruginosa FNCC 0063, Staphylococcus aureus FNCC 6049, Escherichia coli FNCC 0194.…”

SUPLEMENTASI PROBIOTIK Saccharomyces spp.Kb-13 DALAM RANSUM TERHADAP PRODUKSI, KARAKTERISTIK KUALITAS TELUR, DAN KOLESTEROL KUNING TELUR AYAM LOHMANN BROWN

“…Fathi et al (2018) melaporkan bahwa suplementasi probiotik tidak meningkatkan kinerja produksi telur, akan tetapi dapat meningkatkan kualitas kulit telur. Dilaporkan oleh Istiqomah et al (2018) bahwa Saccharomyces cerevisiae berpotensi untuk mengasimilasi kolesterol in vitro.…”

“…Saccharomyces spp juga ditemukan memiliki aktivitas hidrolitik garam empedu yang tinggi, yang bertanggung jawab untuk dekonjugasi garam empedu (Bidura dan Siti 2017). Menurut Istiqomah et al (2018) S. cerevisiae berpotensi untuk mengasimilasi kolesterol in vitro. Kolesterol plasma dan trigliserida berkurang pada ayam petelur yang diberi makanan yang dilengkapi dengan probiotik (Fathi et al, 2018).…”