Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension

Dhand, Chetna; Singh, Surinder P.; Arya, Sunil K.; Datta, Monika; Malhotra, Bansi D.

doi:10.1016/j.aca.2007.09.028

Cited by 109 publications

(52 citation statements)

References 44 publications

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“…The value of K app M was estimated to be 0.21 mM. It was lower than the value of a previous report for the ChOx/PANI-modified electrode (K app M = 0.62 mM) [31] and the ChOx/MWCNT-modified electrode (K app M = 7.17 mM) [32]. This result suggested that ChOx immobilized in nano-Pt/MWCNT-PANI nanocomposite film had a high biological affinity to cholesterol.…”

Section: Electrocatalytic Behavior Of Chox/nano-pt/ Mwcnt-pani/gce Tocontrasting

confidence: 70%

Fabrication of multiwalled carbon nanotube–polyaniline/platinum nanocomposite films toward improved performance for a cholesterol amperometric biosensor

Cheng

Tan

et al. 2015

Biotech and App Biochem

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A simple and high sensitive cholesterol amperometric biosensor, which is based on in situ electropolymerization of multi-walled carbon nanotube-polyaniline (MWCNT-PANI) nanocomposite and electrodeposition of platinum nanoparticle (nano-Pt) films onto the glassy carbon electrode surface for cholesterol oxidase immobilization, was constructed in this study. The preparation process of the modified electrode was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, and chronoamperometry. Because of the synergistic electrocatalytic activity between MWCNT-PANI nanocomposites and nano-Pt, the cholesterol biosensor exhibited an excellent performance with a linear range of 2.0-510.0 µM, a detection limit of 0.8 µM (signal-to-noise ratio = 3), a high sensitivity of 109.9 µA mM , and a short response time within 5 Sec. Moreover, the reproducibility, stability, and selectivity of the biosensor were also investigated.

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Section: Electrocatalytic Behavior Of Chox/nano-pt/ Mwcnt-pani/gce Tocontrasting

confidence: 70%

Fabrication of multiwalled carbon nanotube–polyaniline/platinum nanocomposite films toward improved performance for a cholesterol amperometric biosensor

Cheng

Tan

et al. 2015

Biotech and App Biochem

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“…for the development of various cholesterol biosensors [19][20][21][22][23][24][25][26][27][28][29]. Until now, there are no reports on the use of silk as the immobilization support for cholesterol oxidase (ChOx).…”

Section: Introductionmentioning

confidence: 99%

Silk Mat as Bio-matrix for the Immobilization of Cholesterol Oxidase

Saxena

Goswami

2010

Appl Biochem Biotechnol

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Cholesterol oxidase (ChOx) was covalently immobilized onto the woven silk fiber (silk mat) produced by Antheraea assamensis. The immobilization was done using N-ethyl-N'-(3-dimethylaminopropyl) carbodimide and N-hydroxysuccinimide ligand chemistry. The attachment of ChOx to the silk mat was demonstrated by scanning electron microscopy and activity study. The kinetic studies of the immobilized ChOx were performed by using a biological oxygen monitor. The enzyme loading was found to be 0.046 U cm(-2) of silk mat and the enzyme loading efficiency of the silk mat was estimated to be 70%. Remarkably high storage and operational stability (t(1/2) of initial activities) corresponding to 13 months and 25 numbers of assay (for a period of 6 h), respectively, of the fabricated ChOx electrode were demonstrated.

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“…This emeraldine salt is then treated with a base (NH 4 OH) to convert PANI in its emeraldine base form. For preparation of its colloidal suspension (10 mL of 20 mgL −1 ) in acetonitrile, 200 µL of stock solution (1 mgmL −1 ) of emeraldine base in formic acid is dispersed in 9.8 mL of acetonitrile followed by ultra-sonication for about 1 min (Dhand et al 2007). Thick PANI films from its colloidal suspension are prepared electrophoretically using a two-electrode cell by applying DC voltage.…”

Section: Methodsmentioning

confidence: 99%

Nanostructured conducting polymer based reagentless capacitive immunosensor

Bandodkar

Dhand

Arya

et al. 2009

Biomed Microdevices

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Nanostructured polyaniline (PANI) film electrophoretically fabricated onto indium-tin-oxide (ITO) coated glass plate has been utilized for development of an immunosensor based on capacitance change of a parallel plate capacitor (PPC) by covalently immobilizing anti-human IgG (Anti-HIgG) using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. These fabricated PANI/ITO and Anti-HIgG/PANI/ITO plates have been characterized using scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry and Fourier transform infra-red studies. The capacitance measurements indicate that dielectric medium of this biologically modified PPC (Anti-HIgG/PANI/ITO) is sensitive to HIgG in 5 - 5 x 10(5) ng mL(-1) range and has lower detection limit of 1.87 ng mL(-1). The observed results reveal that this Anti-HIgG modified PPC can be used as a robust, easy-to-use, reagentless, sensitive and selective immunosensor for estimation of human IgG.

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Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension

Cited by 109 publications

References 44 publications

Fabrication of multiwalled carbon nanotube–polyaniline/platinum nanocomposite films toward improved performance for a cholesterol amperometric biosensor

Fabrication of multiwalled carbon nanotube–polyaniline/platinum nanocomposite films toward improved performance for a cholesterol amperometric biosensor

Silk Mat as Bio-matrix for the Immobilization of Cholesterol Oxidase

Nanostructured conducting polymer based reagentless capacitive immunosensor

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