Cholesterol‐conjugated bovine serum albumin nanoparticles as a tamoxifen tumor‐targeted delivery system

Abstract

In the present study, we introduced cholesterol (CLO)‐conjugated bovine serum albumin nanoparticles (BSA NPs) as a new system for indirect targeting drug delivery. Tamoxifen, as an anticancer drug, was loaded on BSA NPs (BSA‐TAX NPs); CLO was then conjugated to the BSA‐TAX NPs surface for the targeted delivery of NPs system, by 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide/N‐hydroxy succinimide carbodiimide chemistry (CLO‐BSA‐TAX NPs). The physicochemical properties, toxicity, in vitro, and in vivo biocompatibility of the BSA NPs system were characterized on cancer cell lines (4T1). The results revealed that the BSA NPs system has a regular spherical shape and negative zeta‐potential values. The drug release of BSA NPs system has shown controlled and pH‐dependent drug release behavior. BSA NPs system was biocompatible but it was potentially toxic on the cancer cell line. The CLO‐BSA‐TAX NPs exhibited higher toxicity against cancer cell lines than other NPs formulation (BSA NPs and BSA‐TAX NPs). It can be concluded that the CLO, as an indirect targeting agent, enhances the toxicity and specificity of NPs system on cancer cell lines. It could potentially be suitable approaches to targeting the tumors in clinical cancer therapy.

Green synthesis of Zn nanoparticles and in situ hybridized with BSA nanoparticles for Baicalein targeted delivery mediated with glutamate receptors to U87‐MG cancer cell lines

“…BSA NPs and BSA‐Ba NPs were synthesized by the modified desolation method. [ 23 ] Briefly, 100 mg BSA was added to 3.5 ml PBS (or 3.5 ml PBS with 0.01% Zn NPs to prepare BSA‐Ba@Zn NPs) and kept at 40–50°C under vigorous stirring for 15 min. Next, 7 ml Ba solution with a concentration of 4 mg ml −1 (Ba/acetone solution) was dropwise added to each of the above solutions under constant stirring and dark conditions.…”

Biogenic and facile synthesis of selenium nanoparticles using Vaccinium arctostaphylos L. fruit extract and anticancer activity against in vitro model of breast cancer

“…The hemocompatibility assay data observed that all samples' hemolytic activity (fruit extract, purified SeNPs, and SS) were concentration‐dependent, without significantly increasing hemolytic activity. Hence, it can be concluded the systems have relatively appropriate biocompatibility, which was approved by previous studies (Gharbavi, Johari, Eslami, et al, 2020; Mittal & Banerjee, 2021). Furthermore, these results are consistent with the MTT assay cytotoxicity of the samples (purified SeNPs and SS) against HEK‐293 normal cells and confirmed that the investigated samples are biologically safe, high biocompatibility, and nontoxic for medicinal applications, which was reported by other studies (Mittal & Banerjee, 2021; Xu, 2020; Yan et al, 2020).…”

NANOG Decoy Oligodeoxynucleotide–Encapsulated Niosomes Nanocarriers: A Promising Approach to Suppress the Metastatic Properties of U87 Human Glioblastoma Multiforme Cells

“…All mice were acclimatized in an animal house under standard conditions for 1 week prior to use. In this assay, eight BALB/c male mice at an average weight of 25–30 g were used according to the OECD guidelines. , This assay was carried out at different NISM@B-DEC concentrations (17.5–1750 mg/kg) to determine the maximum nontoxic concentration of NISM@B-DEC to be used. All animals were monitored to observe any behavioral changes or signs of toxicity after oral administration.…”