Cholesterol-loaded-cyclodextrins and fertility potential of stallions spermatozoa

Spizziri, B.; Fox, Michael H.; Bruemmer, J.E.; Squires, E.L.; Graham, James K.

doi:10.1016/j.anireprosci.2009.08.001

Cited by 67 publications

(47 citation statements)

References 52 publications

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“…Despite the improved freezability of bull semen (MOCÉ; GRAHAM, 2006), the fertility rates were not different between the group in which cholesterol was added to the semen and the control group (PURDY; GRAHAM, 2004). Spizziri et al (2010), observed the improvement of progressive motility of equine semen when cholesterol combined with cyclodextrin was added to the centrifugation extender; however, Oliveira et al (2010) did not observe an improvement in motility after thawing when cholesterol was added to the semen of stallions. In the present study, the addition of a plant substance equivalent of cholesterol, alone or combined with OLA, did not result in significantly different values of motility and vigor in the treatments of Experiments 1 and 3.…”

Section: Discussionmentioning

confidence: 99%

Effect of oleic-linoleic acid and ?-sitosterol to freezing extender of bulls and stallions semen

et al. 2015

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Addition of polyunsaturated fatty acids and/or cholesterol to a freezing diluent can modify the sperm plasma membrane composition, influencing its behavior during cryopreservation, thus, favoring seminal cryoresistance. The present study aimed to evaluate the effects of the addition of oleiclinoleic acid, (OLA); β-sitosterol (β-sit), a plant analog of cholesterol; and OLA + β-sit in combination to a freezing diluent, on the cryopreservation bull and stallion semen. The following variables were analyzed: motility/vigor, plasma and acrosomal membrane integrity (by Trypan Blue/Giemsa staining), mitochondrial activity (by DAB staining), and lipid peroxidation (by a TBARS assays). The lipids were added according to experimental treatments: C -control group, A1 and A2 -OLA at concentrations of 37 μM and 74 μM, B1 and B2 -β-sit at concentrations of 1 µg mL -1 and 2 µg mL -1 ; AB1 and AB2 -OLA 37 μM + β-sit 1 µg mL -1 and OLA 74 μM + β-sit 2 µg mL -1 , respectively. The study was divided into three experiments; in Experiment 1, the concentrations of the groups A1, B1, and AB1 were evaluated, whereas in Experiment 2 the concentrations of the groups A2, B2, and AB2 were analyzed, both experiments were performed with bull semen. We conducted Experiment 3 using equine semen with the addition of lipids at all of the concentrations described. Data were subjected to analysis of variance, using the GLM procedure of SAS, with treatment means compared by Duncan test considering 5% significance. These variables differed significantly after thawing the semen post-collection. However, there was no significant difference between treatments when variables were compared within the same time point, except for Experiment 2, where there was a decrease in motility and vigor decrease postthaw in the groups following β-sit addition (C -51.0 ± 13.7%/2.9 ± 0.4; B2 -35.8 ± 15.8%/2.3 ± 0.6; AB2 -38.5 ± 16.6%/2.5 ± 0.5, respectively; p < 0.05). In conclusion, the tested concentrations of these lipids did not confer greater cryoresistance to the spermatozoa, and were not effective in preserving the structural integrity of plasma and acrosomal membranes after thawing. Furthermore, there was no change in the mitochondrial activity and lipid peroxidation due to lipids addition. Key words: Cholesterol, cryopreservation, PUFAs, spermatozoa ResumoA adição de ácidos graxos poli-insaturados e/ou colesterol ao diluidor pode modificar a composição da membrana plasmática do espermatozoide, influenciando seu comportamento frente à criopreservação e favorecendo, assim, a crioresistência seminal. O presente trabalho teve como objetivo avaliar o efeito da adição do ácido oleico-linoleico (AOL), β-sitosterol (β-sit), um análogo vegetal do colesterol, e da associação AOL + β-sit, ao diluente de congelação de sêmen bovino e equino. As variáveis analisadas foram motilidade/ vigor, integridade das membranas plasmática e acrossomais (Trypan Blue/Giemsa), atividade mitocondrial (DAB) e nível de peroxidação lipídica (TBARS). Os lipídios foram adicionados de acor...

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Section: Discussionmentioning

confidence: 99%

Effect of oleic-linoleic acid and ?-sitosterol to freezing extender of bulls and stallions semen

et al. 2015

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“…Aumento das taxas de sobrevivência a criopreservação foi encontrado em equinos (COMBES et al 2000;MO-ORE;SQUIRES;SPIZZIRI et al, 2010), bovinos (PURDY; GRAHAM, 2004b), bisão americano (HUSSAIN; LESSARD; ANZAR, 2013) ovinos (MORRIER et al, 2004, MOCÉ;GRAHAM, 2010)caprinos (KONYALI, 2009;AMIDI;KHOR, 2010;FAR-SHAD et al 2011) esuínos (GALANTINO-HOMER et al 2006, TOMÁS et al 2013) pela adição de diferentes concentrações de ciclodextrina carregada com colesterol durante o processo de criopreservação, o que permitiu a manutenção de um elevado teor de colesterol na membrana espermática, que por sua vez pode impedir que as células sofram pré-maturação e capacitação precoce, aumentando sua viabilidade.…”

Section: Membrana Plasmática E Colesterolunclassified

“…Em equinos, Oliveira et al (2010) sugeriram que a incorporação de colesterol à membrana plasmática atrasa os processos de capacitação espermática e reduz a reação acrossômica subsequente, o que aumenta o tempo necessário para a capacitação in vivo (SPIZZIRI et al, 2010), fato que prejudicaria a fertilidade, e fundamenta as baixas taxas de gestação (25%/75% e 28,6/15% )em éguas inseminadas com e sem a adição de colesterol ao sêmen, respectivamente (ZANH et al, 2002;SPIZZIRI et al, 2010). Portanto os espermatozoides tratados com CCC podem precisar ser inseminados em um ponto de tempo diferente em relação à ovulação, permitindo uma capacitação mais longa e reação acrossômicaa-dequada (SPIZZIRIet al, 2010).…”

Section: Fertilidade Do Sêmen Tratado Com CCCunclassified

Uso De Colesterol Na Criopreservação Espermática E Fertilidade: Uma Revisão

Boscarato

Martins

Pinho

et al. 2015

ArqVet

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MARTINS, L. F.; PINHO, R. O.; MACEDO, G. G.; ORLANDINI, C. F. Uso de colesterol na criopreservação espermática e fertilidade: uma revisão. Arq. Ciênc. Vet. Zool. UNIPAR, Umuarama, v. 17, n. 2, p. 145-150, abr./ jun. 2014. RESUMO:A criopreservação é um método amplamente utilizado para o armazenamento de células espermáticas em longo prazo. Essa técnica facilita a difusão e a disponibilidade de material genético a ser utilizado tanto na produção in vitro (PIV) como na inseminação artificial, biotecnologia aplicada na maioria das espécies domésticas. Pesquisas têm desenvolvido estratégias para otimizar a utilização do sêmen por meio da redução das injúrias ocorridas durante o processo de criopreservação, e vem conseguindo melhorar os parâmetros espermáticos pós-descongelação com a adição de ciclodextrina carregada com colesterol (CCC) ao sêmen puro ou previamente diluído das diferentes espécies estudadas. Apesar dos resultados promissores, melhor fertilidade do sêmen tratado com CCC tem sido demonstrada somente in vitro, enquanto que in vivo os resultados ainda não são compensatórios, necessitando maiores investigações para tornar viável a aplicação comercial dessa técnica. O objetivo dessa revisão foi compilar alguns resultados dos principais pesquisadores na área, expondo o estado atual e as perspectivas para melhorias da fertilidade do sêmen tratado com CCC. PALAVRAS-CHAVE: Ciclodextrina. Inseminação artificial. Membrana plasmática. Semen. USE OF CHOLESTEROL IN SPERM CRYOPRESERVATION AND FERTILITY: A REVIEWABSTRACT: Cryopreservation is a method widely used for the long-term storage of sperm cells. This technique facilitates the dissemination and availability of genetic material to be used in both in vitro production (IVP) and in artificial insemination, the biotechnology applied to most domestic species. Research has developed strategies to optimize the use of semen through the reduction of injuries occurred during the cryopreservation process, having succeeded in improving post-thaw sperm parameters with the addition of cholesterol-loaded cyclodextrin (CLC) to pure or previously diluted semen samples from the different species studied. Despite the promising results, better fertility of semen treated with CLC has only been demonstrated in vitro, while in vivo results are not yet feasible, requiring further investigation to result in viable commercial application of such technique. The objective of this review is to compile a few results from the leading researchers in the area, exhibiting the current state and prospects for improving the fertility of semen treated with CLC. KEYWORDS: Cyclodextrin. Artificial insemination. Plasma membrane. Semen. USO DE COLESTEROL EN LA CRIOPRESERVACIÓN ESPERMÁTICA Y FERTILIDAD: UNA REVISIÓNRESUMEN: La criopreservación es un método ampliamente utilizado para el almacenamiento de células espermáticas a largo plazo. Esa técnica facilita la difusión y la disponibilidad de material genético a ser utilizado tanto en la producción in vitro (PIV) como en la inseminación artificial, bio...

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“…Inducing cholesterol in sperm plasma membrane with cyclodextrin molecules (CLCs) (44) enhanced the cryotolerance in rams (45), bulls (46)(47)(48), bucks (49), elephants (50), and stallions (51). This might be due to the fact that increased cholesterol in the sperm cell membrane enhanced the osmotic tolerance and inhibited the acrosome reaction (52).…”

Section: β-Cyclodextrinmentioning

confidence: 99%

Cholesterol efflux from sperm: approaches and applications

Naseer¹,

Ahmad²,

Aksoy³

2014

Turk J Vet Anim Sci

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Following ejaculation, sperm appear morphologically mature, but functionally they are inactive. To attain functional capability or capacitation in vivo, sperm must reside in the female reproductive tract for a certain period of time before fertilization. In the female reproductive tract, cholesterol efflux from the sperm plasma membrane is considered vital for the initiation of capacitation. A number of in vitro studies have used biological and nonphysiological means to understand the phenomenon of cholesterol efflux and capacitation. In this review, we present a short overview of the role of cholesterol efflux in regulating sperm capacitation using various agents. This review provides insight to researchers designing new procedures for sperm preparation to be used in assisted reproductive technology.

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Cholesterol-loaded-cyclodextrins and fertility potential of stallions spermatozoa

Cited by 67 publications

References 52 publications

Effect of oleic-linoleic acid and ?-sitosterol to freezing extender of bulls and stallions semen

Effect of oleic-linoleic acid and ?-sitosterol to freezing extender of bulls and stallions semen

Uso De Colesterol Na Criopreservação Espermática E Fertilidade: Uma Revisão

Cholesterol efflux from sperm: approaches and applications

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