Cholesterol was identified as a biomarker in human melanocytic nevi using DESI and DESI/PI mass spectrometry imaging

Qi, K. H.; Lv, Yongmei; Ren, Yun; Wang, Xian; Wu, Lisheng; Wang, Jingwen; Zhang, Xuan; He, Yifu; Zhang, Chenchen; Liu, Chengyuan; Pan, Yang

doi:10.1016/j.talanta.2021.122380

Cited by 17 publications

(14 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All operations were controlled by self-programmed control software based on LabView from National Instruments. The homemade DESI/PI source has been described elsewhere in detail . Two modes, DESI/PI and DESI, were selectively used on-demand by switching on/off an VUV krypton lamp (PKS 106, Heraeus, Cambridge, U.K).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Liu

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

Plant metabolites exhibit a variety of different chemical properties, physiological activities, and biological functions. However, untargeted imaging of highly diverse metabolic profiles is still a great challenge. Here, metabolites in plant leaves were imaged via imprint, followed by desorption electrospray ionization/post-photoionization (imprint DESI/PI) mass spectrometry imaging. In contrast to the traditional imprint DESI method, quite a few metabolites, such as terpenoids, flavonoids, glycosides, alkylphenols, amino acids, phenolic acids, tannins, and lipids, in fresh sage leaves, ginkgo leaves, and tea leaves were well detected and imaged by imprint DESI/PI. More than 80 metabolites were additionally identified, and more than 1 order of magnitude higher signal intensities were obtained for most metabolites in the negative ion mode. By virtue of the significant improvement of coverage and sensitivity of PI, the catechin biosynthesis network in fresh tea leaves could be clearly illustrated, indicating the potential applicability of imprint DESI/PI in exploring the sites and pathways of plant metabolic conversion.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The homemade DESI/PI source has been described elsewhere in detail. 29 Two modes, DESI/PI and DESI, were selectively used on-demand by switching on/ off an VUV krypton lamp (PKS 106, Heraeus, Cambridge, U.K). A DESI sprayer consisted of an inner fused silica capillary (i.d.…”

Section: ■ Introductionmentioning

confidence: 99%

Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Liu

et al. 2022

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…One way to overcome this shortcoming is to introduce a secondary ionization source. Recently, a post‐photoionization source based on a portable krypton discharge lamp was combined with DESI (DESI/PI) in our group to enable the simultaneous ionization of polar and nonpolar compounds in biological tissues at ambient condition 18,19 . The content and type of dopant was found to be essential for the sensitivity of target compounds.…”

Section: Introductionmentioning

confidence: 99%

Effects of dopants in the imaging of mouse brain by desorption electrospray ionization/post‐photoionization mass spectrometry

et al. 2022

J Mass Spectrom

Self Cite

View full text Add to dashboard Cite

Desorption electrospray ionization/post‐photoionization (DESI/PI) is a newly developed ionization method by the combination of DESI and post‐photoionization for the simultaneous imaging of polar and nonpolar compounds in biological tissues. Dopants are of great importance in DESI/PI for the enhancement of signal intensities through ion–molecule reactions. In this work, to evaluate the performance of dopants in DESI/PI, an efficient homogenate model was developed, and four kinds of dopants (toluene, chlorobenzene, bromobenzene, and anisole) were tested using homogenate of mouse brain tissue as target sample. The influences of the dopants on the signal enhancements of different compounds were explained reasonably by the ionization mechanism. Then, the dopants with their optimum volume contents were applied to the mass spectrometry imaging (MSI). For a comprehensive imaging of various compounds with different polarities, methanol/toluene/formic acid (7:3:0.1) was chosen as the best choice. Finally, the stronger quantitative ability of DESI/PI with toluene as dopant for a few compounds in mouse brain tissue was demonstrated.

show abstract

“…In 2010, DESI imaging played a role in cancer diagnostics by discriminating between cancerous and normal tissues of human bladders [ 76 ]. Over the years, it has been proved that DESI-MSI is a powerful tool for in situ analysis of biomolecules such as lipids [ 77 ] and proteins [ 78 ]. Apart from biological samples, various kinds of natural compounds in plant tissues could also be visualized using DESI-MSI [ 30 ].…”

Section: Main Textmentioning

confidence: 99%

The application of mass spectrometry imaging in traditional Chinese medicine: a review

et al. 2022

View full text Add to dashboard Cite

Mass spectrometry imaging is a frontier technique which connects classical mass spectrometry with ion imaging. Various types of chemicals could be visualized in their native tissues using mass spectrometry imaging. Up to now, the most commonly applied mass spectrometry imaging techniques are matrix assisted laser desorption ionization mass spectrometry imaging, desorption electrospray ionization mass spectrometry imaging and secondary ion mass spectrometry imaging. This review gives an introduction to the principles, development and applications of commonly applied mass spectrometry imaging techniques, and then illustrates the application of mass spectrometry imaging in the investigation of traditional Chinese medicine. Recently, mass spectrometry imaging has been adopted to explore the spatial distribution of endogenous metabolites in traditional Chinese medicine. Data collected from mass spectrometry imaging can be further utilized to search for marker components of traditional Chinese medicine, discover new compounds from traditional herbs, and differentiate between medicinal plants that are similar in botanical features. Moreover, mass spectrometry imaging also plays a role in revealing the pharmacological and toxicological mechanisms of traditional Chinese medicine.

show abstract

Cholesterol was identified as a biomarker in human melanocytic nevi using DESI and DESI/PI mass spectrometry imaging

Cited by 17 publications

References 56 publications

Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Enhanced Coverage and Sensitivity of Imprint DESI Mass Spectrometry Imaging for Plant Leaf Metabolites by Post-photoionization

Effects of dopants in the imaging of mouse brain by desorption electrospray ionization/post‐photoionization mass spectrometry

The application of mass spectrometry imaging in traditional Chinese medicine: a review

Contact Info

Product

Resources

About