Choline Oxidase Based Composite ZrO₂@AuNPs with Cu₂O@MnO₂ Platform for Signal Enhancing the Choline Biosensors

Abstract

A novel metal composite material based on zirconium dioxide decorated gold nanoparticles (ZrO2@AuNPs), copper (I) oxide at manganese (IV) oxide (Cu2O@MnO2) and immobilized choline oxidase (ChOx) onto a glassy carbon electrode (GCE) (ChOx/Cu2O@MnO2‐ZrO2@AuNPs/GCE) has been developed for enhancing the electro‐catalytic property, sensitivity and stability of the amperometric choline biosensor. The ChOx/Cu2O@MnO2‐ZrO2@AuNPs/GCE displayed an excellent electrocatalytic response to the oxidation of the byproduct H2O2 from the choline catalyzed reaction, which exhibited a charge transfer rate constant (Ks) of 0.97 s−1, a diffusion coefficient value (D) of 4.50×10−6 cm2 s−1, an electroactive surface area (Ae) of 0.97 cm2 and a surface concentration (γ) of 0.54×10−8 mol cm−2. The modified electrode also provided a wide linear range of choline concentration from 0.5 to 1,000.0 μM with good sensitivity (97.4 μA cm−2 mM−1) and low detection limit (0.3 μM). The apparent Michaelis‐Menten constant was found to be 0.08 mM with Imax of 0.67 μA. This choline biosensor presented high repeatability (%RSD=2.9, n=5), excellent reproducibility (%RSD=2.9, n=5), long time of use (n=28 with %I>50.0 %) and good selectivity without interfering effects from possible electroactive species such as ascorbic acid, aspirin, amoxicillin, caffeine, dopamine, glucose, sucrose and uric acid. This optimal method was successfully applied for choline measurement in prepared human blood samples which demonstrated accurate and excellent reliability in the recovery range from 96.7 to 102.0 %.

A Novel Label‐free Chronoamperometric Immunosensor Based on a Biocomposite Material for Rapid Detection of Carcinoembryonic Antigen

“…Unlike each substance or material modified sensors, various kinds of developed composite materials bring the synergistic phenomena in sensing performance [30][31][32]. As well as our previous research that has demonstrated synergistic effects of the developed multicomposite material solution toward glucose sensing performance [33].…”

Biomedical Applications of Zirconia-Based Nanomaterials: Challenges and Future Perspectives

“…The elaborated fabrication of the composite and the detection mechanism for the designed DNA biosensor are illustrated in Figure 6 [ 116 ]. Various ZrO 2 -based materials are utilized for biosensing applications, such as ZrO 2 /Chitosan composites [ 117 , 118 ], nZrO 2 @PC [ 119 ], CeO 2 -ZrO 2 composites [ 120 ], ZrO 2 @CuNCs [ 121 ], Gox-PLL/RGO-ZrO 2 composites [ 122 ], TiO 2 -ZrO 2 nanocomposites [ 123 ], GQDs@La 3+ @ZrO 2 [ 124 ], ChOx/Cu 2 O@MnO 2 -ZrO 2 @AuNPs/GCE [ 125 ], ZrO 2 NPs in 1-butyl-3-methylimidazolium trifluoroacetate [ 126 ], ZrO 2 -NPs/MacroPSi EGFET [ 127 ], polyaniline–graphene oxide composites decorated with ZrO 2 NPs [ 128 ], etc.…”

Metal and Metal Oxide Based Advanced Ceramics for Electrochemical Biosensors-A Short Review

“…A novel Ga 2 O 3 / lignin and ZrO 2 /lignin hybrid materials have been developed by Jędrzak et al (2019) for the immobilization of glucose oxidase which acts as a biosensor for the detection of glucose. An amperometric choline biosensor was introduced by Ouiram et al (2020) based on zirconium dioxide decorated gold nanoparticles (ZrO 2 @AuNPs), copper (I) oxide at manganese (IV) oxide (Cu 2 O@ MnO 2 ), and immobilized choline oxidase (ChO x ) onto a glassy carbon electrode (GCE) (ChO x /Cu 2 O@MnO 2 -ZrO 2 @AuNPs/GCE) for the detection of choline in blood samples.…”