Choline transport in Pseudomonas aeruginosa

Salvano, Mario A.; Lisa, Teresita A.; Domenech, Carlos E.

doi:10.1007/bf00223517

Cited by 30 publications

(34 citation statements)

References 20 publications

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“…We began to detect choline and GB accumulation by 1 h postaddition, a time course similar to those previously reported (9,33,47). These pools were maintained for at least 12 h during growth on choline as a sole carbon source but were below the detection limit at 24 h (data not shown).…”

Section: Figsupporting

confidence: 77%

Cellular Choline and Glycine Betaine Pools Impact Osmoprotection and Phospholipase C Production in Pseudomonas aeruginosa

Fitzsimmons¹,

Hampel²,

Wargo³

2012

Journal of Bacteriology

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Section: Figsupporting

confidence: 77%

Cellular Choline and Glycine Betaine Pools Impact Osmoprotection and Phospholipase C Production in Pseudomonas aeruginosa

Fitzsimmons¹,

Hampel²,

Wargo³

2012

Journal of Bacteriology

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“…However, its metabolic precursor choline is predicted to be more abundant, predominately as a moiety on larger molecules. Evidence from experimental and natural systems points to rapid bacterial uptake of choline (25)(26)(27)(28). This rapid uptake suggests that, while choline release rates may be high, free choline concentrations may be low.…”

Section: Distribution and Biological Roles Of Choline And Gbmentioning

confidence: 99%

Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Wargo

2013

Appl Environ Microbiol

155

121

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Most sequenced bacteria possess mechanisms to import choline and glycine betaine (GB) into the cytoplasm. The primary role of choline in bacteria appears to be as the precursor to GB, and GB is thought to primarily act as a potent osmoprotectant. Choline and GB may play accessory roles in shaping microbial communities, based on their limited availability and ability to enhance survival under stress conditions. Choline and GB enrichment near eukaryotes suggests a role in the chemical relationships between these two kingdoms, and some of these interactions have been experimentally demonstrated. While many bacteria can convert choline to GB for osmoprotection, a variety of soil-and water-dwelling bacteria have catabolic pathways for the multistep conversion of choline, via GB, to glycine and can thereby use choline and GB as sole sources of carbon and nitrogen. In these choline catabolizers, the GB intermediate represents a metabolic decision point to determine whether GB is catabolized or stored as an osmo-and stress protectant. This minireview focuses on this decision point in Pseudomonas aeruginosa, which aerobically catabolizes choline and can use GB as an osmoprotectant and a nutrient source. P. aeruginosa is an experimentally tractable and ecologically relevant model to study the regulatory pathways controlling choline and GB homeostasis in choline-catabolizing bacteria. The study of P. aeruginosa associations with eukaryotes and other bacteria also makes this a powerful model to study the impact of choline and GB, and their associated regulatory and catabolic pathways, on host-microbe and microbe-microbe relationships.

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“…Included among the pathways found in a Pseudomonas spp. which are regulated by catabolite repression control (CRC) are the inducible enzymes and transport proteins of carbohydrate catabolism (42), histidase (28), amidase (36), protocatechuate dioxygenase (43), alkyl sulfatase (7), urocanase (30), and choline transport (32).…”

mentioning

confidence: 99%

Cloning of a catabolite repression control (crc) gene from Pseudomonas aeruginosa, expression of the gene in Escherichia coli, and identification of the gene product in Pseudomonas aeruginosa

et al. 1991

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Mutants which are defective in catabolite repression control (CRC) of multiple independentdy regulated catabolic pathways have been previously described. The mutations were mapped at 11 min on the Pseudomonas aeruginosa chromosome and designated crc. This report describes the cloning of a gene which restores normal CRC to these Crc-mutants in trans. The gene expressing this CRC activity was subcloned on a 2-kb piece of DNA. When this 2-kb fragment was placed in a plasmid behind a phage T7 promoter and transcribed by T7 RNA polymerase, a soluble protein with a molecular weight (MW) of about 30,000 was produced in Escherichia coli. A soluble protein of identical size was overproduced in a Crc-mutant when it contained the 2-kb fragment on a multicopy plasmid. This protein could not be detected in the mutant containing the vector without the 2-kb insert or with no plasmid. When a 0.3-kb AccI fragment was removed from the crc gene and replaced with a kanamycin resistance cassette, the interrupted crc gene no longer restored CRC to the mutant, and the mutant containing the interrupted gene no longer overproduced the 30,000-MW protein. Pools of intracellular cyclic AMP and the activities of adenylate cyclase and phosphodiesterase were measured in mutant and wild-type strains with and without a plasmid containing the crc gene. No consistent differences between any strains were found in any case. These results provide original evidence for a 30,000-MW protein encoded by crc+ that is required for wild-type CRC in P. aeruginosa and confirms earlier reports that the mode of CRC is cyclic AMP independent in this bacterium.Pseudomonas aeruginosa, like Escherichia coli and other enteric bacteria, is able to preferentially metabolize one carbon source over another when both are present in the growth medium and can repress induction of multiple catabolic pathways by growth on a preferred carbon source (17). Included among the pathways found in a Pseudomonas spp. which are regulated by catabolite repression control (CRC) are the inducible enzymes and transport proteins of carbohydrate catabolism (42), histidase (28), amidase (36), protocatechuate dioxygenase (43), alkyl sulfatase (7), urocanase (30), and choline transport (32).In the enteric bacteria, growth on glucose results in repression of other catabolic pathways, but in P. aeruginosa and other Pseudomonas species, intermediates of the tricarboxylic acid cycle cause repression. CRC has been extensively studied in E. coli (27,35,40,41), and the mechanism of regulation is known to involve a protein (CAP) which, when bound to cyclic AMP (cAMP), interacts with promoter regions to facilitate the binding of RNA polymerase, thereby initiating transcription. In the presence of glucose, the cAMP concentration is lowered, CAP is not bound, and the regulated genes are not transcribed (i.e., they are repressed). The effect of glucose on cAMP pools is mediated by components of the phosphoenolpyruvate: sugar phosphotransferase system (PTS) (29,31). Both genetic and biochemical evidence su...

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Choline transport in Pseudomonas aeruginosa

Cited by 30 publications

References 20 publications

Cellular Choline and Glycine Betaine Pools Impact Osmoprotection and Phospholipase C Production in Pseudomonas aeruginosa

Cellular Choline and Glycine Betaine Pools Impact Osmoprotection and Phospholipase C Production in Pseudomonas aeruginosa

Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Cloning of a catabolite repression control (crc) gene from Pseudomonas aeruginosa, expression of the gene in Escherichia coli, and identification of the gene product in Pseudomonas aeruginosa

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