Cholinephosphotransferase from rabbit lung microsomes. An improved assay and specificity towards exogenous diacylglycerols

Phosphatidylcholine- Lysophosphatidylcholine Cycle Pathway Enzymes in Rabbit Lung. II. Marked Differences in the Effect of Gestational Age on Activity Compared to the CDP-Choline Pathway

“…Transacylation in this pathway in the developing lung has only recently been studied (15) by following the incorporation of IysoPC into PC using developing rabbit lung slices. The acylation of IysoPC in the developing rabbit lung microsomes was also recently reported (19).…”

Lung Lipid Composition and Surfactant Biology

“…However, the experimental evidence in support of this pathway in the lung was largely (Van Golde, 1976), although not entirely (Morgan, 1969), based on the misidentification of phosphatidylglycerol as phosphatidyl-N-dimethylethanolamine (Hallman and Gluck, 1974;Rooney et al, 1974). Subsequent studies found no evidence in support of the methylation pathway of phosphatidylcholine synthesis in the normal lung (Epstein and Farrell, 1975;Rooney and Motoyama, 1976), but it may be active in conditions of choline deficiency (Yost et al, Human (Nikolasev, 1974) Rhesus Monkey (Kerr and Helmuth, 1975) Rat (Okano et al, 1978) Squirrel (Melling and Keough, 1981) Bovine (Okano and Akino, 1979) Guinea Pig (Okano and Akino, 1979) Pig (Okano and Akino, 1979) Pig (Body, 1971) Rabbit (Okano and Akino, 1979) Rat (Okano et al, 1978) Human (Hanamura et al, 1980) Rat (Okano et al, 1978) Rat (Okano et al, 1978) Bovine (Okano and Akino, 1979) Guinea Pig (Okano and Akino, 1979) Pig (Okano and Akino, 1979) Rabbit (Okano and Akino, 1979) Rat (Okano et al, 1978) Percent of the Total Phospholipid 1986). Because this pathway is not operative in the normal lung and choline is not synthesized by mammals, dietary choline is essential for de novo synthesis of phosphatidylcholine.…”

The Action of Lung Lysosomal Phospholipases on Dipalmitoyl Phosphatidylcholine and its Significance for the Synthesis of Pulmonary Surfactant

“…The de novo pathways of phosphatidylcholine synthesis in the lung produce principally unsaturated molecules (32,33,35) which must be converted to the dipalmitoyl species by a remodeling process (6,37). This starts with phospholipase A2 action on the unsaturated lipid yielding 1-palmitoyl-2-lysophosphatidylcholine which can be turned into the required product by the introduction of a palmitoyl residue at the vacant 2-position of molecule.…”