Cholinergic and adrenergic receptors on mouse cardiocytes in vitro

Lane, Mary-Anne; Sastre, Antonio; Law, M.; Salpeter, Miriam M.

doi:10.1016/0012-1606(77)90213-5

Cited by 53 publications

(23 citation statements)

References 25 publications

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“…These results are in agreement with previous studies on beating heart cells (40,141). Beat-to-beat regulation in the heart is thought to be controlled by the entry of extracellular calcium which triggers calcium release from the sarcoplasmic reticulum (142).…”

Section: Discussionsupporting

confidence: 83%

Studies of adrenergic hormone action and protein thiolation in beating rat heart cells

Fisher

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Section: Discussionsupporting

confidence: 83%

Studies of adrenergic hormone action and protein thiolation in beating rat heart cells

Fisher

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“…The quantity k1/k2 is the amount bound per unit area per unit concentration at equilibrium. The number of binding sites for the antagonist [3H]alprenolol in cultured heart muscle cells has been stated to be at least 30-50 per /tm2 and possibly many times that number (Lane, Sastre & Salpeter, 1977); we shall take a value of 200. In the absence of definite information we assume half the receptors (i.e.…”

Section: Diffusionmentioning

confidence: 99%

Synaptic delay in the heart: an ionophoretic study.

Hill-Smith¹,

Purves²

1978

The Journal of Physiology

104

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SUMMARY1. Neurotransmitters were applied ionophoretically to spontaneously beating clusters of ventricular muscle cells cultured from neonatal rats.2. Acetylcholine or its analogue carbachol produced hyperpolarization and decreased the rate of spontaneous beating. These responses had minimum latencies of about 250 msec and total durations of 6-12 sec.3. Noradrenaline, adrenaline or isoprenaline increased the rate of spontaneous beating. The minimum latency for this effect was 3-6 sec. Following a single brief pulse the rate remained elevated for 2 min or more.4. Chronotropic responses of intact atria from adult rats to stimulation of the autonomic nerves were of similar time course to responses of the cultured muscle cells.5. Calculations based on the theory of diffusion showed that access of drugs to their receptors could not be rate-limiting for the observed responses, unless a diffusion barrier of rather special properties was postulated. A number of other explanations for the long latencies have been ruled out; these are most likely to be due to some physical or chemical process occurring in or under the cell membrane.6. Attempts to mimic responses to catecholamines by intracellular application of cyclic AMP were unsuccessful, perhaps because the release of nucleotide from the pipettes was insufficient. A theoretical treatment suggests that ionophoretic efflux of anions might be greatly diminished by the opposing electro-osmotic flux.

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“…A recent study using a rapid-flow technique suggested that cultured heart cells were capable of an immediate response to as little as 1 nM AcCho (4). Autoradiographic observations suggest that the potent muscarinic antagonist quinuclidinyl benzilate (QNB) binds specifically to cultured mouse heart cells (5). However, studies of properties of direct ligand binding to muscarinic receptors in heart cell cultures have not been reported.…”

mentioning

confidence: 99%

Properties of muscarinic acetylcholine receptors in heart cell cultures

Galper

Smith

1978

Proc. Natl. Acad. Sci. U.S.A.

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The binding of acetylcholine to receptors in the intact heart causes a decrease in the frequency (chronotropic effect) and force (ionotropic effect) of contraction. The studies reported here demonstrate a chronotropic response of cultured embryonic chicken heart cells to the muscarinic agonist carbamoylcholine. ¶tis response is markedly decreased after a 3-hr incubation with 0.1 mM carbamoylcholine. In order to determine whether agonist-induced alterations in muscarinic receptors were responsible for this decrease, we studied the effects of incubation with carbamoylcholine on the binding of the 3'H-labeled muscarinic The binding of acetylcholine (AcCho) to muscarinic receptors of the myocardial cell causes a decrease in the frequency and force of contraction. Evidence for the presence of muscarinic AcCho receptors in cultured heart cells is based primarily on studies of the chronotropic effects of muscarinic agonists (1-3). Although early studies suggested that chicken heart cells in culture were insensitive to the effects of f,-adrenergic and cholinergic agonists (1), other groups have demonstrated negative chronotropic effects of cholinergic agonists after 15-30 min of incubation (2, 3). A recent study using a rapid-flow technique suggested that cultured heart cells were capable of an immediate response to as little as 1 nM AcCho (4). Autoradiographic observations suggest that the potent muscarinic antagonist quinuclidinyl benzilate (QNB) binds specifically to cultured mouse heart cells (5). However, studies of properties of direct ligand binding to muscarinic receptors in heart cell cultures have not been reported.After exposure to agonists, many types of hormone receptors rapidly become desensitized (6). Exposure of embryonicThe publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact. chicken heart to the muscarinic agonist carbamoylcholine (CbmCho) causes slowing of beating rate, but a return to control beating rate occurs within 1-2 min (7). More prolonged exposure of hormone-sensitive tissues to f3-adrenergic and prostaglandin agonists markedly decreases the number of sites available for specific binding of radiolabeled ligands (8-10). In those systems in which hormones produce increased adenylate cyclase activity, incubation with agonists also results in "partial desensitization" of the adenylate cyclase response (8)(9)(10). Whether this apparent decrease in receptor number and the accompanying decrease in adenylate cyclase response are related to the physiologic desensitization to agonists is not clear.Previous studies utilizing direct binding of [3H]QNB (11) defined the properties of [3H]QNB binding to muscarinic receptors in embryonic chicken heart (7) and, recently, in rabbit, rat, and guinea pig heart (12). In the present investigation we have extended these techniques to study the properties of muscarinic receptors in heart cell cultures an...

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Cholinergic and adrenergic receptors on mouse cardiocytes in vitro

Cited by 53 publications

References 25 publications

Studies of adrenergic hormone action and protein thiolation in beating rat heart cells

Studies of adrenergic hormone action and protein thiolation in beating rat heart cells

Synaptic delay in the heart: an ionophoretic study.

Properties of muscarinic acetylcholine receptors in heart cell cultures

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