Cholinergic molecules modify the in vitro behavior of cells from early embryos of the medaka Oryzias latipes, a teleost fish

Fluck, Richard A.; Wynshaw‐Boris, Anthony; Schneider, L.M.

doi:10.1016/0306-4492(80)90054-4

Cited by 15 publications

(7 citation statements)

References 15 publications

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“…The same localization was reported by (Fluck et al, 1980; Fluck and Shih, 1981) in Oryzias latipes mesoderm cell cultures. After these discoveries, the presence of AChE activity is considered as a good marker of migrating neural crest cells (Le Douarin, 1986) and of poly ingressing epiblast cells during gastrulation (Weinberger et al, 1984).…”

Section: Non-neuromuscular Roles Of the Molecules Related To The Cholsupporting

confidence: 88%

“…A number of research groups found active ChE localized in extra-neuromuscular sites of different animal embryos, including invertebrate (Drews, 1975), and vertebrate embryos (Drews, 1975; Fluck et al, 1980; Minganti and Falugi, 1980; Minganti et al, 1981), and in developing buds of vegetal organisms (Raineri and Modenesi, 1986, 1988). They suggested that this AChE activity might be related to “embryonic” functions: cell migration (Drews, 1975) and cell-to-cell communication related to positional information exchange, such as the one between apical ectodermal ridge (AER) and mesenchyme during the limb development (Drews, 1975; Falugi and Raineri, 1985).…”

Section: Non-neuromuscular Roles Of the Molecules Related To The Cholmentioning

confidence: 99%

“…3.1.1.7) has been found during gametes interaction (Piomboni et al, 2001; Angelini et al, 2004, 2005; Falugi et al, 2008), embryonic development, in temporal and spatial windows related to differentiation and cell migration (Drews, 1975; Fluck et al, 1980; Falugi, 1993). …”

Section: Introductionmentioning

confidence: 99%

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Early appearance and possible functions of non-neuromuscular cholinesterase activities

Falugi¹,

Aluigi²

2012

Front. Mol. Neurosci.

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The biological function of the cholinesterase (ChE) enzymes has been studied since the beginning of the twentieth century. Acetylcholinesterase plays a key role in the modulation of neuromuscular impulse transmission in vertebrates, while in invertebrates pseudo cholinesterases are preeminently represented. During the last 40 years, awareness of the role of ChEs role in regulating non-neuromuscular cell-to-cell interactions has been increasing such as the ones occurring during gamete interaction and embryonic development. Moreover, ChE activities are responsible for other relevant biological events, including regulation of the balance between cell proliferation and cell death, as well as the modulation of cell adhesion and cell migration. Understanding the mechanisms of the regulation of these events can help us foresee the possible impact of neurotoxic substances on the environmental and human health.

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Section: Non-neuromuscular Roles Of the Molecules Related To The Cholsupporting

confidence: 88%

Section: Non-neuromuscular Roles Of the Molecules Related To The Cholmentioning

confidence: 99%

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Early appearance and possible functions of non-neuromuscular cholinesterase activities

Falugi¹,

Aluigi²

2012

Front. Mol. Neurosci.

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“…The primary toxicity of these substances is due to an "irreversible" inhibition of acetylcholinesterase (AChE), the lytic enzyme of the cholinergic neurotransmission system. In the past few years, there has been increasing evidence that AChE may display a role in regulating embryonic development and neurogenesis (Drews 1975;Fluck et al 1980;Buznikov et al 1997;Grifman et al 1998). It is now well known that neurotransmitter molecules produce signals that may be translated into cell-to-cell and cell-environment interactions driving development.…”

Section: Introductionmentioning

confidence: 98%

The sea urchin, Paracentrotus lividus, embryo as a “bioethical” model for neurodevelopmental toxicity testing

Aluigi

Angelini

Corte³

et al. 2008

Cell Biol Toxicol

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Presently, a large effort is being made worldwide to increase the sustainability of industrial development, while preserving not only the quality of the environment but also that of animal and human life. In this work, sea urchin early developmental stages were used as a model to test the effects of the organophosphate pesticide (diazinon) on the regulation of gene expression by immunohistochemical localization of the human regulatory protein against the human OTX2. Egg exposure to diazinon did not affect fertilization; however, at concentrations 10(-5)-10(-6) M, it did cause developmental anomalies, among which was the dose-dependent alteration of the intracellular distribution of a regulatory protein that is immunologically related to the human OTX2. The severe anomalies and developmental delay observed after treatment at 10(-5) M concentration are indicators of systemic toxicity, while the results after treatment at 10(-6) M suggest a specific action of the neurotoxic compound. In this second case, exposure to diazinon caused partial delivery of the protein into the nuclei, a defective translocation that particularly affected the blastula and gastrula stages. Therefore, the possibility that neurotoxic agents such as organophosphates may damage embryonic development is taken into account. Specifically, the compounds are known to alter cytoplasmic dynamics, which play a crucial role in regulating the distribution of intracellular structures and molecules, as well as transcription factors. Speculatively, basing our assumptions on Fura2 experiments, we submit the hypothesis that this effect may be due to altered calcium dynamics, which in turn alter cytoskeleton dynamics: the asters, in fact, appear strongly positive to the OTX2 immunoreaction, in both control and exposed samples. Coimmunoprecipitation experiments seem to supply evidence to the hypothesis.

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“…In one case, the consequence of the depletion of AChE activity (due to professional OP exposure) in the maternal blood, lasting throughout the pregnancy, caused the birth of a baby with only one eye, brain and heart anomalies, who died after some days (Romero et al, 1989). During the last decades, neurotransmitter systems (Buznikov, 1990;Buznikov and Shmukler, 1996) and in particular the cholinergic systems (Drews, 1975;Minganti et al, 1981;Fluck et al, 1980 andFalugi, 1993) have been found responsible for cell interactions leading early development. In particular, molecules belonging to the cholinergic system, whose presence and amount is regulated by AChE and BChE activity, were found to exert a neurotrophic effect (Filogamo & Marchisio, 1971), and a strong input to neurogenesis and axon differentiation (Biagioni et al, 2001).…”

Section: Reproductive and Developmental Anomaliesmentioning

confidence: 99%

Cholinergic Pesticides

Falugi¹,

Rakonczay²,

Thielecke³

et al. 2011

Pesticides - The Impacts of Pesticides Exposure

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Cholinergic molecules modify the in vitro behavior of cells from early embryos of the medaka Oryzias latipes, a teleost fish

Cited by 15 publications

References 15 publications

Early appearance and possible functions of non-neuromuscular cholinesterase activities

Early appearance and possible functions of non-neuromuscular cholinesterase activities

The sea urchin, Paracentrotus lividus, embryo as a “bioethical” model for neurodevelopmental toxicity testing

Cholinergic Pesticides

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