Cholinoreceptor neurons of the snail: Identification, plasticity, and its regulation by opioids and second messengers

Pivovarov, A. S.

doi:10.1007/bf02355664

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…In the common snail, depolarization of neurons RPa4, RPa3, and LPa3 is induced by selective n-and selective m-cholinomimetics [5]. Thus, the n-and m-cholinomceptors have similar functions.…”

Section: As Regards the Identification Of Cholinoreceptors Inmentioning

confidence: 98%

Characteristics of cholinoreceptors on identified TAN neurons of the ground snailAchatina fulica

Stepanov

Losev

2000

Neurosci Behav Physiol

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The characteristics of cholinoreceptors located on neurons TAN1, TAN2, and TAN3 of the ground snail Achatina fulica were studied by incubation of the central ganglia in a bath with cholinotropic preparations during intracellular recording of background neuron spike activity. Acetylcholine, nicotine, the selective n-cholinoreceptor agonist suberyldicholine, and the selective n-cholinoreceptor agonist 5-methylfurmethide concentration-dependently inhibited background spike activity to the level of complete blockade at concentrations of 500 microM. The m-cholinoblocker metamizil (500 microM) completely prevented the inhibitory activity of concentrations of 5-methylfurmethide of up to 500 microM. The central n-cholinoblocker etherophen (500 microM) completely blocked the inhibitory activity of 500 microM suberyldicholine. However, metamizil and etherophen added separately only partially decreased the inhibitory effects of acetylcholine but could not completely block the effect of acetylcholine. At the same time, mixtures of metamizil and etherophen (500 microM each) completely blocked the inhibition of background spike activity induced by acetylcholine. These results show that both classes of cholinoreceptors act on TAN neurons in the same direction.

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Section: As Regards the Identification Of Cholinoreceptors Inmentioning

confidence: 98%

Characteristics of cholinoreceptors on identified TAN neurons of the ground snailAchatina fulica

Stepanov

Losev

2000

Neurosci Behav Physiol

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“…The local application of acetylcholine to specific loci of the membrane induces depolarizational transmitter potentials that are blocked by tubocurarine or atropine, respectively. In addition, some segments of the membrane are sensitive to serotonin [8,20].…”

Section: The Command Neuronmentioning

confidence: 99%

“…The excitation of the modulatory neurons intensifies the efficiency of the synapses of detectors which are formed on a command neuron [9,30]. In addition, the modulatory neurons, by secreting a neuropeptide into the intercellular milieu, activate the intracellular mechanisms of the command neurons, turning on the latent pacemaker mechanism [20,28].…”

Section: The Conceptual Reflex Arcmentioning

confidence: 99%

The architecture of the reflex arc

1994

Neurosci Behav Physiol

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The identification of local detectors, command neurons, and modulator neurons has opened up the possibility of the structural identification of individual synaptic contacts supporting the functioning of the reflex arc. Nonassociative plasticity (habituation and sensitization) are [sic] realized at the level of the receptors and potential-dependent calcium channels through dephosphorylation-phosphorylation of receptor and channel proteins. Associative plasticity (the development and extinction of the conditioned reflex) includes two levels of regulation: short-term (through dephosphorylation-phosphorylation of receptor and channel proteins) and long-term (through the expression of genes coding structural and translocational genes). Its selectivity is an extremely important characteristic of associative plasticity. The mechanism of associative plasticity is based on the principles of the Hebb plastic synapse, supplemented by indication of the role of nonspecific (modulating) influences.

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“…Repeated applications of acetylcholine (ACh) to the command neurons of H. lucorum lead to a short-term depression of an acetylcholine-induced inward current (ACh-current). Postsynaptic mechanisms are responsible for this effect because ACh release from the ionophoretic pipette was constant (Pivovarov 1992(Pivovarov , 1995(Pivovarov , 2001. These changes, which can be maintained from a few minutes to a few hours, are responsible for synaptic plasticity.…”

Section: Introductionmentioning

confidence: 99%

Habituation-Like Decrease of Acetylcholine-Induced Inward Current in Helix Command Neurons: Role of Microtubule Motor Proteins

2015

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The role of kinesin and dynein microtubuleassociated molecular motors in the cellular mechanism of depression of acetylcholine-induced inward chloride current (ACh-current) was examined in command neurons of land snails (Helix lucorum) in response to repeated applications of ACh to neuronal soma. This pharmacological stimulation imitated the protocol of tactile stimulation evoking behavioural habituation of the defensive reaction.In this system, a dynein inhibitor (erythro-9-(2-hydroxy-3-nonyl)adenine, 50 lM) decreased the ACh-current depression rate. Kinesin Eg5 inhibitors (Eg5 inhibitor III, 10 lM and Eg5 inhibitor V, trans-24, 15 lM) reduced the degree of current depression, and Eg5 inhibitor V also reduced the initial rate of depression. The results of electrophysiological experiments in combination with mathematical modelling provided evidence of the participation of dyneins and kinesin Eg5 proteins in the radial transport of acetylcholine receptors in command neurons of H. lucorum in the cellular analogue of habituation. Furthermore, these results suggest that the reciprocal interaction between dynein and kinesin proteins located on the same vesicle can lead to reverse their usual direction of transport (dyneins-in exocytosis and kinesin Eg5-in endocytosis).Keywords EHNA Á Eg5-inhibitor-III Á Eg5-inhibitor-V trans-24 Á Short-term depression of ACh-induced chloride current Á Command neurons Á Helix lucorum Á Habituation

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Cholinoreceptor neurons of the snail: Identification, plasticity, and its regulation by opioids and second messengers

Cited by 3 publications

References 17 publications

Characteristics of cholinoreceptors on identified TAN neurons of the ground snailAchatina fulica

Characteristics of cholinoreceptors on identified TAN neurons of the ground snailAchatina fulica

The architecture of the reflex arc

Habituation-Like Decrease of Acetylcholine-Induced Inward Current in Helix Command Neurons: Role of Microtubule Motor Proteins

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