Chondrocyte migration in an acellular tissue‐engineered cartilage substitute

Nachtsheim, Julia; Dursun, Gözde; Markert, Bernd; Stoffel, Marcus

doi:10.1002/pamm.201800425

Cited by 3 publications

(9 citation statements)

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“…Our results show that the chondrocytes migrate from the matrix into the cell-free implants. This confirms previous findings in the literature [8][10] [11]. However, a number of questions concerning cell phenotype and function remain to be answered.…”

Section: Discussionsupporting

confidence: 91%

“…In vitro models play a key role in studying cellular processes [8]. In this study, we have established a new in vitro cellular model consisting of chondrocyte-seeded matrix and cellfree collagen I based implants in a PDMS mold to study the chondrocyte colonisation into cell-free implants.…”

Section: Discussionmentioning

confidence: 99%

“…The load cell placed under the cultivation chamber records the force during experiments. The cultivation chamber including the cell-seeded matrix and the cell-free implants was manufactured from autoclavable materials (PEEK(Polyether ether ketone) and glass) and possesses two filter caps for gas exchange [8] (see Figure 2). Cyclic compression, with a strain of 5% and frequency of 0.1 Hz, was applied to dynamically cultured constructs without any break while statically cultured constructs were not exposed to any cyclic compression.…”

Section: Mechanical Stimulation In a Compression Bioreactormentioning

confidence: 99%

“…Previous studies have already shown the potential of cell-free collagen type-I implants to trigger the cellular in-growth and create a good quality repair tissue in short and long terms [5] [6]. However, based on the knowledge of authors, there are no studies applying mechanical stimulation to promote and accelerate the chondrocyte migration into the cell-free implants [7] [8].…”

Section: Introductionmentioning

confidence: 99%

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Experimental Study on Cell-free Approach for Articular Cartilage Treatment

Dursun

Markert

Stoffel

2019

Current Directions in Biomedical Engineering

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Cell-free based approaches are introduced as a promising treatment method for articular cartilage. The success of this method requires cell colonisation from resident tissue into cell-free implants. The objective of our study is to promote the cell colonisation into cell-free collagen I based implants by mechanical stimulation. Therefore, a new in vitro cellular model consisting chondrocyte-seeded matrix and cell-free implants was developed in a polydimethylsiloxan (PDMS) mold. These constructs were cultured under dynamic and static culture conditions. For the dynamic culture, we have developed an in-house bioreactor system where both the load and the deformation applied to the specimen are recorded. Cyclic compression, with a strain of 5% and frequency of 0.1 Hz, was applied to constructs without any break. At the end of three days of dynamic and static cultivation, the cell-free implants were seperated from cell-seeded matrix and cultured in a petri dish three days long. Afterwards, they were analysed using fluoresence dyes. The microscopic assessment indicated that there was a cell migration into the cell-free implants which were cultured dynamically.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Mechanical Stimulation In a Compression Bioreactormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Experimental Study on Cell-free Approach for Articular Cartilage Treatment

Dursun

Markert

Stoffel

2019

Current Directions in Biomedical Engineering

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“…To our knowledge, this is the first study suggesting mobilization of MSCs from a low compartment to another at the top against gravity, induced by biomechanical stimulation in vitro. Migration of chondrocytes under mechanical stimulation has been previously stated [40][41][42], and migration of MSCs under mechanical stimulation was addressed in the past by Ode and collaborators, demonstrating that loading hampered the mobilization of MSCs in bone healing context [43] using a bioreactor system previously described [44]. Both bioreactors were able to apply load on a scaffold.…”

Section: Discussionmentioning

confidence: 92%

Bioreactor for mobilization of mesenchymal stem/stromal cells into scaffolds under mechanical stimulation: Preliminary results

et al. 2020

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OPEN ACCESS Citation: Gamez C, Schneider-Wald B, Schuette A, Mack M, Hauk L, Khan AuM, et al. (2020) Bioreactor for mobilization of mesenchymal stem/ stromal cells into scaffolds under mechanical stimulation: Preliminary results. PLoS ONE 15(1): e0227553. https://doi.org/10.1371/journal. ResultsThe bioreactor was able to stimulate the scaffolds and the cells for 24.4 (±1.7) hours, exerting compression with vertical displacements of 185.8 (±17.8) μm and a force-amplitude of 1.87 (±1.37; min 0.31, max 4.42) N. Our results suggest that continuous mechanical stimulation hampered the viability of the cells located at the cell reservoir when comparing to intermittent mechanical stimulation (34.4 ± 2.0% vs. 66.8 ± 5.9%, respectively).Functionalizing alginate scaffolds with laminin-521 (LN521) seemed to enhance the mobilization of cells from 48 (±21) to 194 (±39) cells/mm 3 after applying intermittent mechanical loading. ConclusionThe bioreactor presented here was able to provide mechanical stimulation that seemed to induce the mobilization of MSCs into LN521-alginate scaffolds under an intermittent loading regime.Bioreactor for mobilization of MSCs under mechanical stimulation PLOS ONE | https://doi.

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Designing of an Advanced Compression Bioreactor with an Implementation of a Low-Cost Controlling System Connected to a Mobile Application

et al. 2021

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(1) Background: Bioreactors mimic the natural environment of cells and tissues by providing a controlled micro-environment. However, their design is often expensive and complex. Herein, we have introduced the development of a low-cost compression bioreactor which enables the application of different mechanical stimulation regimes to in vitro tissue models and provides the information of applied stress and strain in real-time. (2) Methods: The compression bioreactor is designed using a mini-computer called Raspberry Pi, which is programmed to apply compressive deformation at various strains and frequencies, as well as to measure the force applied to the tissue constructs. Besides this, we have developed a mobile application connected to the bioreactor software to monitor, command, and control experiments via mobile devices. (3) Results: Cell viability results indicate that the newly designed compression bioreactor supports cell cultivation in a sterile environment without any contamination. The developed bioreactor software plots the experimental data of dynamic mechanical loading in a long-term manner, as well as stores them for further data processing. Following in vitro uniaxial compression conditioning of 3D in vitro cartilage models, chondrocyte cell migration was altered positively compared to static cultures. (4) Conclusion: The developed compression bioreactor can support the in vitro tissue model cultivation and monitor the experimental information with a low-cost controlling system and via mobile application. The highly customizable mold inside the cultivation chamber is a significant approach to solve the limited customization capability of the traditional bioreactors. Most importantly, the compression bioreactor prevents operator- and system-dependent variability between experiments by enabling a dynamic culture in a large volume for multiple numbers of in vitro tissue constructs.

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Chondrocyte migration in an acellular tissue‐engineered cartilage substitute

Cited by 3 publications

References 4 publications

Experimental Study on Cell-free Approach for Articular Cartilage Treatment

Experimental Study on Cell-free Approach for Articular Cartilage Treatment

Bioreactor for mobilization of mesenchymal stem/stromal cells into scaffolds under mechanical stimulation: Preliminary results

Designing of an Advanced Compression Bioreactor with an Implementation of a Low-Cost Controlling System Connected to a Mobile Application

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