Chondrocyte Phenotype in Engineered Fibrous Matrix Is Regulated by Fiber Size

Li, Wan‐Ju; Jiang, Yi; Tuan, Rocky S.

doi:10.1089/ten.2006.12.1775

Cited by 229 publications

(131 citation statements)

References 39 publications

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“…Besides chemical properties, it has been established that the size of nanofibers regulates adhesion, proliferation, and differentiation of NIH-3T3 fibroblasts, chondrocytes, and osteoblasts. [27][28][29][30] The representative mechanical parameters of CPSAPAni/PLCL nanofibers calculated from stress-strain curves are presented in Figure 3. Overall, PACL-1 shows the highest tensile strength, Young's modulus, and elongation at break.…”

Section: Resultsmentioning

confidence: 99%

Development of Electroactive and Elastic Nanofibers that contain Polyaniline and Poly(L‐lactide‐co‐ε‐caprolactone) for the Control of Cell Adhesion

Jeong¹,

Jun²,

Choi³

et al. 2008

Macromolecular Bioscience

175

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In this work, electrically conductive polyaniline (PAni) doped with camphorsulfonic acid (CPSA) is blended with poly(L-lactide-co-epsilon-caprolactone) (PLCL), and then electrospun to prepare uniform nanofibers. The CPSA-PAni/PLCL nanofibers show a smooth fiber structure without coarse lumps or beads and consistent fiber diameters (which range from 100 to 700 nm) even with an increase in the amount of CPSA-PAni (from 0 to 30 wt.-%). However, the elongation at break decreases from 391.54 +/- 9.20% to 207.85 +/- 6.74% when 30% of CPSA-PAni is incorporated. Analysis of the surface of the nanofibers demonstrates the presence of homogeneously blended CPSA-PAni. Most importantly, a four-point probe analysis reveals that electrical properties are maintained in the nanofibers where the conductivity is significantly increased from 0.0015 to 0.0138 S x cm(-1) when the nanofibers are prepared with 30% CPSA-PAni. The cell adhesion tests using human dermal fibroblasts, NIH-3T3 fibroblasts, and C2C12 myoblasts demonstrate significantly higher adhesion on the CPSA-PAni/PLCL nanofibers than pure PLCL nanofibers. In addition, the growth of NIH-3T3 fibroblasts is enhanced under the stimulation of various direct current flows. The CPSA-PAni/PLCL nanofibers with electrically conductive properties may potentially be used as a platform substrate to study the effect of electrical signals on cell activities and to direct desirable cell function for tissue engineering applications.

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Section: Resultsmentioning

confidence: 99%

Development of Electroactive and Elastic Nanofibers that contain Polyaniline and Poly(L‐lactide‐co‐ε‐caprolactone) for the Control of Cell Adhesion

Jeong¹,

Jun²,

Choi³

et al. 2008

Macromolecular Bioscience

175

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“…Electrospun membranes possess high porosity, large surface area and maximum interconnectivity of pores with a non-woven nanofibrous structure [17][18][19]. The fibers within the resulting scaffold more closely mimic the size and structure of the native extracellular matrix than other previously investigated scaffolds, which can lead to beneficial cellular interactions [20,21].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of PU/PEGMA crosslinked hybrid scaffolds by in situ UV photopolymerization favoring human endothelial cells growth for vascular tissue engineering

Wang

Feng

et al. 2012

J Mater Sci: Mater Med

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Poly(ethylene glycol) methacrylate (PEGMA) was introduced into a polyurethane (PU) solution in order to prepare electrospun scaffold with improving the biocompatibility by electrospinning technology for potential application as small diameter vascular scaffolds. Crosslinked electrospun PU/PEGMA hybrid nanofibers were fabricated by a reactive electrospinning process with N,N'-methylenebisacrylamide as crosslinker and benzophenone as photoinitiator. The photoinduced polymerization and crosslinking reaction took place simultaneously during the electrospinning process. The electrospinning solutions with various weight ratios of PU/PEGMA were successfully electrospun. No significant difference in the scaffold morphology was found by SEM when PEGMA content was <20 wt%. The crosslinked fibrous scaffolds of PU/PEGMA exhibited higher mechanical strength than the pure PU scaffold. The hydrophilicity of scaffolds was controlled by varying the PU/PEGMA weight ratio. The tissue compatibility of electrospun nanofibrous scaffolds were tested using human umbilical vein endothelial cells (HUVECs). Cell morphology and cell proliferation were measured by SEM, fluorescence microscopy and thiazolyl blue assay (MTT) after 1, 3, 7 days of culture. The results indicated that the cell morphology and proliferation on the crosslinked PU/PEGMA scaffolds were better than that on the pure PU scaffold. Furthermore, the appropriate hydrophilic surface with water contact angle in the range of 55-75° was favorable of improvement the HUVECs adhesion and proliferation. Cells seeded on the crosslinked PU/PEGMA (80/20) scaffolds infiltrated into the scaffolds after 7 days of growth. These results indicated the crosslinked electrospun PU/PEGMA nanofibrous scaffolds were potential substitutes for artificial vascular scaffolds.

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“…Further studies demonstrated that the maintenance of a chondrocytic phenotype is enhanced on nanofi bers with dimensions similar to the native ECM, e.g., in the nano region, when compared to micro-sized fi bers. [ 41 ] A number of preceding studies, including ours, were focused on mimicking the fi brous protein structure of ECM utilizing different polymer-based fi brous scaffolds. [ 42 ] Up to this point, pellet cultures-thought of as the golden standard in cartilage engineering [ 35 ] was considered to work as positive control.…”

Section: Earlymentioning

confidence: 99%

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen‐Based Nanofibers

Forget¹,

Awaja

Gugutkov

et al. 2016

Macromolecular Bioscience

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Mimicking the complex intricacies of the extra cellular matrix including 3D configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. This study shows that human adipose derived mesenchymal stem cells (hADMSCs) establishes significant chondrogenic differentiation and may generate quality cartilage when cultured on 2D and randomly oriented fibrinogen/poly‐lactic acid nanofibers compared to 3D sandwich‐like environments. The adhering cells show well‐developed focal adhesion complexes and actin cytoskeleton arrangements confirming the proper cellular interaction with either random or aligned nanofibers. However, quantitative reverse transcription‐polymerase chain reaction analysis for Collagen 2 and Collagen 10 genes expression confirms favorable chondrogenic response of hADMSCs on random nanofibers and shows substantially higher efficacy of their differentiation in 2D configuration versus 3D constructs. These findings introduce a new direction for cartilage tissue engineering through providing a simple platform for the routine generation of transplantable stem cells derived articular cartilage replacement that might improve joint function.

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Chondrocyte Phenotype in Engineered Fibrous Matrix Is Regulated by Fiber Size

Cited by 229 publications

References 39 publications

Development of Electroactive and Elastic Nanofibers that contain Polyaniline and Poly(L‐lactide‐co‐ε‐caprolactone) for the Control of Cell Adhesion

Development of Electroactive and Elastic Nanofibers that contain Polyaniline and Poly(L‐lactide‐co‐ε‐caprolactone) for the Control of Cell Adhesion

Fabrication of PU/PEGMA crosslinked hybrid scaffolds by in situ UV photopolymerization favoring human endothelial cells growth for vascular tissue engineering

Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen‐Based Nanofibers

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