Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads

Ma, Hsiao-Li; Hung, Shih‐Chieh; Lin, Shan-Yang; Chen, Yuh‐Lien; Lo, Wai‐Hee

doi:10.1002/jbm.a.10370

Cited by 193 publications

(158 citation statements)

References 42 publications

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“…Chondrocytes encapsulated in alginate remain differentiated, as opposed to cells cultured in monolayers, which become fibroblastic (Hauselmann et al, 1994). Moreover, MSCs in beads of this polymer that were cultured in medium containing TGF or BMP-2, -6, or -9 underwent chondrogenic differentiation (Ma et al, 2003;Majumdar et al, 2001). Bovine MSCs seeded in alginate beads also maintained a spherical form and increased the expression of chondrocyte-specific genes under TGF b1 treatment.…”

Section: Discussionmentioning

confidence: 99%

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis

Bosnakovski

Mizuno

Kim

et al. 2006

Biotech & Bioengineering

444

322

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Bone marrow mesenchymal stem cells (MSCs) are candidate cells for cartilage tissue engineering. This is due to their ability to undergo chondrogenic differentiation after extensive expansion in vitro and stimulation with various biomaterials in three-dimensional (3-D) systems. Collagen type II is one of the major components of the hyaline cartilage and plays a key role in maintaining chondrocyte function. This study aimed at analyzing the MSC chondrogenic response during culture in different types of extracellular matrix (ECM) with a focus on the influence of collagen type II on MSC chondrogenesis. Bovine MSCs were cultured in monolayer as well as in alginate and collagen type I and II hydrogels, in both serum free medium and medium supplemented with transforming growth factor (TGF) b1. Chondrogenic differentiation was detected after 3 days of culture in 3-D hydrogels, by examining the presence of glycosaminoglycan and newly synthesized collagen type II in the ECM. Differentiation was most prominent in cells cultured in collagen type II hydrogel, and it increased in a timedependent manner. The expression levels of the of chondrocyte specific genes: sox9, collagen type II, aggrecan, and COMP were measured by quantitative ''Real Time'' RT-PCR, and genes distribution in the hydrogel beads were localized by in situ hybridization. All genes were upregulated by the presence of collagen, particularly type II, in the ECM. Additionally, the chondrogenic influence of TGF b1 on MSCs cultured in collagenincorporated ECM was analyzed. TGF b1 and dexamethasone treatment in the presence of collagen type II provided more favorable conditions for expression of the chondrogenic phenotype. In this study, we demonstrated that collagen type II alone has the potential to induce and maintain MSC chondrogenesis, and prior interaction with TGF b1 to enhance the differentiation.

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Section: Discussionmentioning

confidence: 99%

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis

Bosnakovski

Mizuno

Kim

et al. 2006

Biotech & Bioengineering

444

322

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“…Alginate is broadly used in in vitro experiments and provides an easy to control 3D cell culture environment. Previous studies have demonstrated differentiation of MSCs in alginate to NP-like cells [25,26] but direct comparison with medically approved, although for different applications, materials was not done before. Since a range of these medical devices are made of biological extracellular matrix molecules, we hypothesized that they may support equally to or better than alginate chondrogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…To induce MSC chondrogenesis growth factors such as transforming growth factor (TGF)-b1, 2 or 3 [18], bone morphogenetic protein-2 [19], -4 [20], -7 [21] or -14 (GDF-5) [22], have been described to facilitate MSCs differentiation, however the aim of this project was to focus on the differences caused by variety of matrixes. In particular, many studies already described culture of MSCs embedded in several types of scaffolds, such as agarose gels [23], alginate beads [24][25][26], synthetic polymers [27,28] and other biomaterials [29][30][31][32]. These studies provide clear evidence that there is a need of a cell support for enhancing cell-cell and cell-matrix interactions and creating a 3D environment.…”

Section: Introductionmentioning

confidence: 99%

Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

et al. 2011

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Introduction Cell-based therapies for regeneration of the degenerated intervertebral disc (IVD) are an alternative to current surgical intervention. Mesenchymal stem cells (MSCs), in combination with a scaffold, might be ideal candidates for regenerating nucleus pulposus (NP), the pressure-distributing part of the IVD. While the use of growth factors for MSCs differentiation currently receives major attention, in this study we compare the performance of sponge-like matrixes in supporting cell differentiation into NP-like cells. Materials and methods Four types matrixes approved as medical devices for other applications were tested as scaffolds for MSCs: two made of equine or porcine collagen, one of gelatin and one of chitosan. Bone marrowderived human MSCs were seeded in these scaffolds or embedded in alginate, as a three-dimensional control. After five weeks in culture, NP-like differentiation of the cellscaffold constructs was analyzed by qRT-PCR, histology, total DNA quantification, proteoglycan accumulation and immunohistochemistry. Results MSCs in collagen matrixes and gelatin produced more mRNA and proteins of the chondrogenic markers collagen type I, collagen type II (COL2) and aggrecan (ACAN), when compared with cells embedded in alginate or chitosan. Proteoglycan accumulation and cell survival were also higher in collagen and gelatin matrixes. Gene expression results were also confirmed by histological and immunohistochemical staining. In contrast to alginate control, the gene expression of the undesired bone marker osteopontin was lower in all tested groups. In porcine collagen supports, MSC expression ratio between COL2/ ACAN closely resembled the expression of nucleus pulposus cells, but gene expression of recently described NP markers keratin19, PAX1 and FOXF1 was lower. Conclusions Collagen supports provide a readily available, medically approved and effective scaffold for chondrogenic differentiation in vitro, but the phenotype of differentiated MSCs is not yet completely equivalent to that of NP cells.

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“…The immobilization of cells in particles is widely discussed in tissue engineering; the possibility to encapsulate non-human cells reduces the host's immune system response, facilitating transplantation as an alternative to the limited donor tissues available [1]. The technique of cell encapsulation is being used for the production of "Bio artificial Pancreas" [2], immunobarrier for islet cells transplantation [2][3][4], and the encapsulation of stem cells [5,6]. Encapsulation of living cells was also suggested for the production of chemicals such as alcohol, organic acids, steroids, antibiotics, vaccines [7].…”

Section: Introductionmentioning

confidence: 99%

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

Morelli

Holdich

Dragosavac

2017

Journal of Membrane Science

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Citation: MORELLI, S., HOLDICH, R. and DRAGOSAVAC, M., 2017. Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification. Journal of Membrane Science, 524, Additional Information:• This paper was accepted for publication in the journal Journal of This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. conditions. The liquid drops were loaded with increasing amount of yeast (3.14 x 10 7 to 3.14 x 10 8 cells/ mL). The stability and uniformity of the emulsions was independent of the cell concentration. PTFE coated hydrophobic membrane produced smaller W/O drops compared to FAS coated membranes. The liquid polymeric droplets were solidified in solid particles using thermal gelation and/or ionic crosslinking, obtaining yeast encapsulated particles sized ~100 µm.The pH sensitive polymer, Eudragit S100, was used as a coating to create gastro resistant particles suitable for intestinal-colonic targeted release. Viability of the released yeast cells was demonstrated using fluorescence probes and checking cell glucose metabolism with time. AbbreviationsW/O emulsion, water in oil

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Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads

Cited by 193 publications

References 42 publications

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: Influence of collagen type II extracellular matrix on MSC chondrogenesis

Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification

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