Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells induced by acellular cartilage sheets

Xue, Ji Xin; Gong, Yi; Zhou, Guang; Liu, Wei; Cao, Yilin; Zhang, Wen Jie

doi:10.1016/j.biomaterials.2012.04.054

Cited by 97 publications

(89 citation statements)

References 22 publications

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“…Therefore, these matrices can potentially direct cell behavior in tissue-engineered constructs. CDM scaffolds have previously been reported to be beneficial for the chondrogenic differentiation of MSCs [39,40]. The present study gives a further insight in the performance of MSCs and chondrocytes in relation to CDM.…”

Section: Discussionmentioning

confidence: 74%

Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles

Gawlitta²,

et al. 2015

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Section: Discussionmentioning

confidence: 74%

Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles

Gawlitta²,

et al. 2015

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“…Synthetic polymers may have problems with retention and degradation in situ, 35 while biological materials carry the risk of infection and immunological reactions. 36,37 Therefore, new approaches that avoid the use of such scaffolds have been developed, including the use of thermos-responsive polymeric surfaces, 38 acellular sheet technology, 39 and electrospun sheet technology. 40 Unfortunately, these technologies are not easy to manipulate.…”

Section: Discussionmentioning

confidence: 99%

Transplantation of Scaffold-Free Cartilage-Like Cell-Sheets Made from Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair

et al. 2016

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Objective The object of this study was to determine culture conditions that create stable scaffold-free cartilage-like cell-sheets from human bone marrow–derived mesenchymal stem cells (hBMSCs) and to assess their effects after transplantation into osteochondral defects in nude rats. Design (Experiment 1) The hBMSCs were harvested from 3 males, the proliferative and chondrogenic capacities were assessed at passage 1, and the cells were expanded in 3 different culture conditions: (1) 5% fetal bovine serum (FBS), (2) 10% FBS, and (3) 5% FBS with fibroblast growth factor 2 (FGF-2). The cells were harvested and made chondrogenic pellet culture. The cell proliferation rate, glycosaminoglycan/DNA ratio, and safranin-O staining intensity of pellets cultured condition 3 were higher than those of conditions 1 and 2. (Experiment 2) The hBMSCs were expanded and passaged 3 times under culture condition 3, and fabricate the cell-sheets in chondrogenic medium either with or without FBS. The cell-sheets fabricated with FBS maintained their size with flat edges. (Experiment 3) The cell-sheets were transplanted into osteochondral defects in nude rats. Histological analysis was performed at 2, 4, and 12 weeks after surgery. Results The osteochondral repair was better after sheet transplantation than in the control group and significantly improved Wakitani score. Immunostaining with human-specific vimentin antibody showed that the transplanted cells became fewer and disappeared at 12 weeks. Conclusions These results indicate that culture with FGF-2 may help to quickly generate sufficient numbers of cells to create stable and reliable scaffold-free cartilage-like cell-sheets, which contribute to the regeneration of osteochondral defects.

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“…M esenchymal stem/stromal cells (MSCs) are multipotent cells, which are able to give rise to fibroblast-like colonies [colony-forming units-fibroblasts (CFU-F)] [1,2] and to differentiate into mesodermally derived tissues, including bone, cartilage, muscle, stromal cells, tendon, and connective tissue [3][4][5][6][7]. Cultured MSCs are well-characterized spindleshaped cells that express a panel of key surface markers, including CD29, CD73, CD90, CD105, CD106, CD140b, and CD166, but lack CD31, CD45, CD34, CD133, and class II MHC expression [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Prospective Isolation of Mesenchymal Stem Cells from Human Bone Marrow Using Novel Antibodies Directed Against Sushi Domain Containing 2

Sivasubramaniyan

Harichandan

Schumann

et al. 2013

Stem Cells and Development

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Several strategies have been developed to facilitate the prospective isolation of bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs) based on the selective expression or absence of surface markers. Recently, we described the monoclonal antibodies W3D5 and W5C5, which selectively react with BM-MSCs, but not with hematopoietic cells. Both antibodies showed an identical reactivity pattern, indicating that they may recognize the same molecule. To identify the cognate antigen, cultured MSCs were sorted for cells expressing either very high levels of W5C5/W3D5 antigen or for cells which were negative for this antigen. Further processing of these cells for microarray analysis revealed a 20-fold enrichment of the type 1 integral membrane protein Sushi domain containing 2 (SUSD2) in the in W5C5(+) subset. To confirm the identity of the W5C5/W3D5 antigen to SUSD2, HEK293 cells were transfected with the full-length coding sequence of human SUSD2 followed by reactivity analysis of W5C5 and W3D5 antibodies with the transfected line. Flow cytometric analysis showed that both antibodies selectively recognized HEK293/huSUSD2 cells, but not the parental cell line. In line with this, SUSD2 siRNA treatment of SUSD2(+) WERI-RB-1 retinoblastoma cells reduced the expression levels of W3D5 and W5C5 antigens to ~39% and 37%, respectively. Finally, FACSorting and colony assays revealed that only SUSD2(+), but not SUSD2(-) BM cells give rise to colony-forming units-fibroblasts and are able to differentiate into osteoblasts, adipocytes, and chondrocytes. In conclusion, we identified SUSD2 as a novel and specific marker for the prospective isolation of BM-MSCs.

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Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells induced by acellular cartilage sheets

Cited by 97 publications

References 22 publications

Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles

Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles

Transplantation of Scaffold-Free Cartilage-Like Cell-Sheets Made from Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair

Prospective Isolation of Mesenchymal Stem Cells from Human Bone Marrow Using Novel Antibodies Directed Against Sushi Domain Containing 2

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