Chondroitin sulfate epitope (WF6) and hyaluronic acid as serum markers of cartilage degeneration in patients following anterior cruciate ligament injury

Pruksakorn, Dumnoensun; Rojanasthien, S.; Pothacharoen, Peraphan; Luevitoonvechkij, Sirichai; Wongtreratanachai, Prasit; Ongchai, Siriwan; Kongtawelert, Prachya

doi:10.1016/j.jsams.2008.02.003

Cited by 15 publications

(19 citation statements)

References 8 publications

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“…Two veterinarians recorded the severity of clinical signs at each visit (weekly) using a clinical scoring system applied from Nganvongpanit [27] (Table 2). All veterinarians were blinded to the study.…”

Section: Methodsmentioning

confidence: 99%

“…All veterinarians were blinded to the study. During the experiment, 3 mL of blood was collected from the cephalic vein of each dog for evaluation of the level of biomarkers for OA weekly [21,27]. …”

Section: Methodsmentioning

confidence: 99%

“…Quantitative ELISA for the epitope recognized by the monoclonal antibody WF6 was modified from a previous study [21,25,27]. The antibody was specific for intact chondroitin sulfate chains and showed no interaction with other sulfated glycosaminoglycans, hyaluronan, or other polyanions such as DNA, RNA or dextran sulfate.…”

Section: Methodsmentioning

confidence: 99%

“…OPD substrate was then used for detection of the conjugated antibody, and plate reading was carried out as described above. The concentration of HA in samples was calculated from the standard curve [21,25,27]. …”

Section: Methodsmentioning

confidence: 99%

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Effects of one-time and two-time intra-articular injection of hyaluronic acid sodium salt after joint surgery in dogs

Nganvongpanit

Boonsri²,

Sripratak³

et al. 2013

J Vet Sci

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Thirty-one dogs with patellar luxation (grades 2 and 3) were categorized into three groups. Group 1 (G.1; n = 12) had sodium hyaluronate (SHA) intra-articularly injected into the stifle joint that received surgery. Group 2 (G.2; n = 10) received SHA twice: first after surgery and then 1 week later. Group 3 (G.3; n = 9) served as a control, without injection. Blood was collected before injection and then once a week for 4 weeks after injection for evaluation of chondroitin sulfate (CS-WF6) and hyaluronan (HA). The results revealed significantly (p < 0.05) improved clinical scores by the end of week 4 in G.1 and G.2 relative to G.3; however, there was no significant difference between G.1 and G.2. There was a significant decrease (p < 0.05) in serum CS-WF6 levels beginning at week 2 in G.1 and G.2. At weeks 3 and 4, serum HA in G.1 and G.2 differed from that in G.3 (p < 0.05). No significant difference (p > 0.05) was observed in serum biomarkers between G.1 and G.2. In conclusion, intra-articular injection with SHA after joint surgery may improve homeostasis of the joint, retarding the process of OA.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of one-time and two-time intra-articular injection of hyaluronic acid sodium salt after joint surgery in dogs

Nganvongpanit

Boonsri²,

Sripratak³

et al. 2013

J Vet Sci

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“…Serum is easier to obtain than synovial fluid and is of particular interest in biomarker analysis for screening purposes and for longitudinal studies. In a recent study, the concentration of W6F (a chondroitin sulfate epitope) was higher in the serum of ACL-injured subjects compared with uninjured controls [78]. Appropriate analysis of serum biomarkers probably cannot be performed in isolation because the detected concentrations represent processes within the entire body, while synovial fluid is more reflective of metabolism within the affected joints.…”

Section: Mri Of Bone Marrow Menisci and Synovium In Pre-osteoarthritismentioning

confidence: 99%

Early diagnosis to enable early treatment of pre-osteoarthritis

et al. 2012

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Osteoarthritis is a prevalent and disabling disease affecting an increasingly large swathe of the world population. While clinical osteoarthritis is a late-stage condition for which disease-modifying opportunities are limited, osteoarthritis typically develops over decades, offering a long window of time to potentially alter its course. The etiology of osteoarthritis is multifactorial, showing strong associations with highly modifiable risk factors of mechanical overload, obesity and joint injury. As such, characterization of pre-osteoarthritic disease states will be critical to support a paradigm shift from palliation of late disease towards prevention, through early diagnosis and early treatment of joint injury and degeneration to reduce osteoarthritis risk. Joint trauma accelerates development of osteoarthritis from a known point in time. Human joint injury cohorts therefore provide a unique opportunity for evaluation of pre-osteoarthritic conditions and potential interventions from the earliest stages of degeneration. This review focuses on recent advances in imaging and biochemical biomarkers suitable for characterization of the pre-osteoarthritic joint as well as implications for development of effective early treatment strategies.

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Walking Speed As a Potential Indicator of Cartilage Breakdown Following Anterior Cruciate Ligament Reconstruction

Pietrosimone

Blackburn

Harkey

et al. 2016

Arthritis Care & Research

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Objective. To determine whether or not self-selected walking speed associates with serum biomarkers of cartilage (collagen and proteoglycan) breakdown in anterior cruciate ligament reconstructed (ACLR) individuals. Methods. Twenty individuals with a history of a primary unilateral ACLR participated in this cross-sectional study. Resting blood was collected from each participant prior to completing 5 walking gait trials at a self-selected comfortable speed. Walking speed was evaluated in a 3-dimensional motion capture laboratory and determined from the velocity of the pelvic center of mass. Sera were assessed for collagen type II cleavage product (C2C) and proteoglycan (aggrecan) concentrations using commercially available specific enzyme-linked immunosorbent assays. Pearson's product-moment (r) and Spearman's (r) correlations were used to evaluate associations between walking speed and biomarkers of cartilage breakdown metabolism. Partial correlations were used to determine whether covariates influenced associations between walking speed and biomarkers of cartilage breakdown. Results. ACLR individuals with a slower walking speed demonstrated higher concentrations of serum C2C (r 5 20.52, P 5 0.02), while there was no significant association between walking speed and aggrecan concentrations (r 5 20.29, P 5 0.31). After accounting for the variance associated with stance phase duration, ACLR individuals with a slower walking speed still demonstrated greater serum C2C concentrations (partial r 5 20.53, P 5 0.02). Conclusion. ACLR individuals who habitually walk slower may experience a greater degree of collagen breakdown, suggesting that walking speed may be a future useful clinical indicator for identifying individuals with higher levels of cartilage breakdown and preradiographic osteoarthritic joint changes.

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Chondroitin sulfate epitope (WF6) and hyaluronic acid as serum markers of cartilage degeneration in patients following anterior cruciate ligament injury

Cited by 15 publications

References 8 publications

Effects of one-time and two-time intra-articular injection of hyaluronic acid sodium salt after joint surgery in dogs

Effects of one-time and two-time intra-articular injection of hyaluronic acid sodium salt after joint surgery in dogs

Early diagnosis to enable early treatment of pre-osteoarthritis

Walking Speed As a Potential Indicator of Cartilage Breakdown Following Anterior Cruciate Ligament Reconstruction

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