Chorionic and amniotic membrane-derived stem cells have distinct, and gestational diabetes mellitus independent, proliferative, differentiation, and immunomodulatory capacities

Chen, Liyun; Merkhan, Marwan M.; Forsyth, Nicholas R.; Wu, Pensée

doi:10.1016/j.scr.2019.101537

Cited by 29 publications

(30 citation statements)

References 49 publications

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“…During embryogenesis, the extraembryonic mesoderm surrounding the amniotic cavity gives rise to the placental membrane, composed of the amniotic and chorionic mesoderm [9]. Chorionic MSCs (CMSCs) are derived from the chorion and share common MSC characteristics and multipotency [10,11]. As CMSCs emerge during pregnancy, their characteristics are highly likely to be affected by pregnancy complications.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling reveals altered biological characteristics of chorionic stem cells from women with gestational diabetes

et al. 2020

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Background: Gestational diabetes (GDM) is a common complication of pregnancy. The impact of pregnancy complications on placental function suggests that extraembryonic stem cells in the placenta may also be affected during pregnancy. Neonatal tissue-derived stem cells, with the advantages of their differentiation capacity and noninvasive isolation processes, have been proposed as a promising therapeutic avenue for GDM management through potential cell therapy approaches. However, the influence of GDM on autologous stem cells remains unclear. Thus, studies that provide comprehensive understanding of stem cells isolated from women with GDM are essential to guide future clinical applications. Methods: Human chorionic membrane-derived stem cells (CMSCs) were isolated from placentas of healthy and GDM pregnancies. Transcriptional profiling was performed by DNA microarray, and differentially regulated genes between GDM-and Healthy-CMSCs were used to analyse molecular functions, differentiation, and pathway enrichment. Altered genes and biological functions were validated via real-time PCR and in vitro assays. Results: GDM-CMSCs displayed, vs. Healthy-CMSCs, 162 upregulated genes associated with increased migration ability, epithelial development, and growth factor-associated signal transduction while the 269 downregulated genes were strongly linked to angiogenesis and cellular metabolic processes. Notably, significantly reduced expression of detoxification enzymes belonging to the aldehyde dehydrogenase gene families (ALDH1A1/1A2, ALDH2, ALDH3) accounted for downregulation across several metabolic pathways. ALDH activity and inhibitor assays indicated that reduced gene expression of ALDHs affected ALDH enzymatic functions and resulted in oxidative stress dysregulation in GDM-CMSCs. Conclusion: Our combined transcriptional analysis and in vitro functional characterisation have provided novel insights into fundamental biological differences in GDM-and Healthy-CMSCs. Enhanced mobility of GDM-CMSCs may promote MSC migration toward injured sites; however, impaired cellular metabolic activity may negatively affect any perceived benefit.

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Section: Introductionmentioning

confidence: 99%

Transcriptional profiling reveals altered biological characteristics of chorionic stem cells from women with gestational diabetes

et al. 2020

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“…Inquiring into the immunomodulatory properties of perinatal MSC from normal and complicated pregnancies could help dissect their role in disease and, ultimately, in fetal-maternal tolerance. In addition, the epigenetic state of fetal genes has been shown to be altered during gestational diabetes mellitus (GDM) [159], and CM from hAMSC and hCMSC from healthy and GDM pregnancies show an equivalent immunoregulatory effect on modulating T cell (Jurkat) proliferation and cytokine secretion [160]. Similarly, both hAMSC and hCMSC from normal and GDM pregnancies have the ability to affect macrophage cytokine secretion, but hCMSC from GDM pregnancies had a reduced effect on macrophage regulation as compared with those from normal pregnancies [160].…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the epigenetic state of fetal genes has been shown to be altered during gestational diabetes mellitus (GDM) [159], and CM from hAMSC and hCMSC from healthy and GDM pregnancies show an equivalent immunoregulatory effect on modulating T cell (Jurkat) proliferation and cytokine secretion [160]. Similarly, both hAMSC and hCMSC from normal and GDM pregnancies have the ability to affect macrophage cytokine secretion, but hCMSC from GDM pregnancies had a reduced effect on macrophage regulation as compared with those from normal pregnancies [160]. Likewise, hAMSC from preeclamptic placenta feature similar immune modulatory [49] and cytokine profile [161] to cells from normal pregnancies, thus, suggesting a potential for these cells in efficiently counteracting the inflammatory environment and, ultimately, contributing to fetal survival.…”

Section: Resultsmentioning

confidence: 99%

Perinatal Mesenchymal Stromal Cells and Their Possible Contribution to Fetal-Maternal Tolerance

Magatti

Stefani

Papait

et al. 2019

Cells

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During pregnancy, a successful coexistence between the mother and the semi-allogenic fetus occurs which requires a dynamic immune system to guarantee an efficient immune protection against possible infections and tolerance toward fetal antigens. The mechanism of fetal-maternal tolerance is still an open question. There is growing in vitro and in vivo evidence that mesenchymal stromal cells (MSC) which are present in perinatal tissues have a prominent role in generating a functional microenvironment critical to a successful pregnancy. This review highlights the immunomodulatory properties of perinatal MSC and their impact on the major immune cell subsets present in the uterus during pregnancy, such as natural killer cells, antigen-presenting cells (macrophages and dendritic cells), and T cells. Here, we discuss the current understanding and the possible contribution of perinatal MSC in the establishment of fetal-maternal tolerance, providing a new perspective on the physiology of gestation.

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“…The result was confirmed by submitting two additional cultures to the adipogenic medium. Although trilineage differentiation is one of the minimal criteria to define MSCs (Dominici et al 2006), little or no adipogenic differentiation potential has already been described for cells isolated from different tissues, such as murine (Nadri and Soleimani 2007) and human amniotic fluid (Chen et al 2019) or human umbilical chord (Kern et al 2006;Shetty et al 2010). In these cases, lack of adipogenic differentiation was suggested to be related to the ontogenetic age of the cells.…”

Section: Discussionmentioning

confidence: 99%

Coronary corium, a new source of equine mesenchymal stromal cells

et al. 2020

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Mesenchymal stromal cells (MSCs) have attracted great attention for therapeutic applications. Since cells derived from different tissues have different properties, using the right tissue source may impact their efficiency in regenerative medicine. This study describes for the first time the isolation and characterization of MSCs derived from the equine coronary corium, which may be useful for treating diseases such as laminitis. Seven coronary corium samples were used for isolation of cells (ccMSCs). Adherent cells were characterized for morphology, immunophenotype, proliferation and differentiation potential, in vitro migration and colony-forming capacity. The cells displayed the characteristic fibroblastoid morphology, with population doubling time increasing until passage 7 and reaching a plateau in passage 10. Cells were negative for CD14 and CD45, and positive for CD73 and CD90. ccMSCs showed chondrogenic and osteogenic, but not adipogenic differentiation, and migrated with nearly total closing of the empty area in 48 h, in the scratch assay. The clonogenic potential was in average 18% to 23%. This study describes for the first time the establishment of mesenchymal stromal cell cultures from the equine coronary corium. The results are similar to MSCs isolated from many other equine tissues, except for restricted differentiation potential. As coronary corium stem cell regulation may contribute to the pathogenesis of equine chronic laminitis, the use of ccMSCs in cell therapy for this significantly debilitating disease should be further investigated.

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Chorionic and amniotic membrane-derived stem cells have distinct, and gestational diabetes mellitus independent, proliferative, differentiation, and immunomodulatory capacities

Cited by 29 publications

References 49 publications

Transcriptional profiling reveals altered biological characteristics of chorionic stem cells from women with gestational diabetes

Transcriptional profiling reveals altered biological characteristics of chorionic stem cells from women with gestational diabetes

Perinatal Mesenchymal Stromal Cells and Their Possible Contribution to Fetal-Maternal Tolerance

Coronary corium, a new source of equine mesenchymal stromal cells

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