Choroidal endothelial cell activation in age-related macular degeneration

Skeie, Jessica M.

doi:10.17077/etd.rqzkgl09

Cited by 4 publications

(3 citation statements)

References 195 publications

(147 reference statements)

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“…Abnormal morphology was the first indication that the commercial “choroidal endothelial” cell line (cCEC) was not of endothelial origin. Human primary choroidal endothelial cells harvested from donor tissues (hCEC) typically maintain a “dark-centered, cobblestone-like” monolayer at confluence 42 – 44 ( Fig. 2 A), yet cCEC did not present with these hallmarks ( Fig.…”

Section: The Need For Authentic Human Primary Choroidal Cell Lines: Wmentioning

confidence: 99%

Cell Line Authentication in Vision Research and Beyond: A Tale Retold

Peavey

Malek

2020

Invest. Ophthalmol. Vis. Sci.

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We live in an age where new technologies, and organizations involved in the distribution of biological materials, such as cell culture lines, have eased accessibility to a variety of in vitro models, developed, and/or harvested from different sources. In translational and basic ophthalmology research, in vitro assays are an essential component to discovery and preclinical studies. It is, therefore, of utmost importance for vision researchers to be cognizant of the risks surrounding the use of newly developed cell culture models and how scientific integrity could be impacted when standard operating procedures are not followed for cell line validation and identification. Herein, we discuss authentication challenges we faced when we obtained a newly marketed human choroidal endothelial cell line for vision research, and outline our process of validating and characterizing primary human choroidal endothelial cell lines in the laboratory.

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Section: The Need For Authentic Human Primary Choroidal Cell Lines: Wmentioning

confidence: 99%

Cell Line Authentication in Vision Research and Beyond: A Tale Retold

Peavey

Malek

2020

Invest. Ophthalmol. Vis. Sci.

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“…Pericytes, fibroblasts, and RPE cells are the major groups of cells that contaminate primary ocular EC cultures [84,85]. Of note, ocular ECs may differentiate into fibroblastic cells after several subcultures, and the use of primary eye ECs for in vitro studies should be performed with early culture passages [86].…”

Section: Ecs In Angiogenesismentioning

confidence: 99%

The Different Facades of Retinal and Choroidal Endothelial Cells in Response to Hypoxia

Mammadzada

André

2018

IJMS

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Ocular angiogenic diseases, such as proliferative diabetic retinopathy and neovascular age-related macular degeneration, are associated with severe loss of vision. These pathologies originate from different vascular beds, retinal and choroidal microvasculatures, respectively. The activation of endothelial cells (EC) plays pivotal roles in angiogenesis, often triggered by oxygen deficiency. Hypoxia-inducible factors in ECs mediate the transcription of multiple angiogenic genes, including the canonical vascular endothelial growth factors. ECs show notable heterogeneity in function, structure, and disease, therefore the understanding of retinal/choroidal ECs (REC; CEC) biochemical and molecular responses to hypoxia may offer key insights into tissue-specific vascular targeting treatments. The aim of this review is to discuss the differences spanning between REC and CEC, with focus on their response to hypoxia, which could provide innovative and sustainable strategies for site specific targeting of ocular neovascularization.

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“…of new blood vessels into the retina. This pathology may be initiated by proteins capable of activating endothelial cells to become angiogenic or infl ammatory, later causing them to grow abnormally [5].…”

Section: Introductionmentioning

confidence: 99%

Significant improvement of Age-Related Macular Degeneration with Melanin Precursors (QIAPI 1®). Case Report

Solois¹,

Carmen²,

P³

2021

J Clin Res Ophthalmol

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Age-related macular degeneration is the fi rst cause of blindness in cold countries in the last 70 years ago or more. In spite expensive therapeutics strategies like intraocular injections, so far, the incidence and prevalence of ARMD cannot be improved signifi cantly. In this case report, we demonstrate that melanin is a fundamental part of macular biology and once is attended the recovery both anatomical and functional is signifi cant.

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Choroidal endothelial cell activation in age-related macular degeneration

Cited by 4 publications

References 195 publications

Cell Line Authentication in Vision Research and Beyond: A Tale Retold

Cell Line Authentication in Vision Research and Beyond: A Tale Retold

The Different Facades of Retinal and Choroidal Endothelial Cells in Response to Hypoxia

Significant improvement of Age-Related Macular Degeneration with Melanin Precursors (QIAPI 1®). Case Report

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