Chromatographic Characterization of a New Cationic β-CD Based Stationary Phase Prepared by Dynamic Coating

Kučerová, Gabriela; Kalíková, Květa; Procházková, Hana; Popr, Martin; Jindřich, Jindřich; Coufal, Pavel; Tesařová, Eva

doi:10.1007/s10337-016-3050-z

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…The dynamic chiral coating process consists of the attachment of the Hp-β-CD chiral selector on a PAC column coated with C18 groups through non-covalent coating. To immobilize the selector, the 10mM Hp-β-CD was dissolved in phosphate buffer (25 mM, pH 4) and pumped through the column [15,28] at a column temperature that was fixed at 30 ºC. The chiral selector solution was recycled through the column at a constant flow rate of 0.25 µl/min Changes in the absorbance of the eluent were followed by UV detection.…”

Section: Chiral Selector Immobilization Via Physical Absorptionmentioning

confidence: 99%

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Simultaneous enantioseparation of nonsteroidal anti-inflammatory drugs by a one-dimensional liquid chromatography technique using a dynamically coated chiral porous silicon pillar array column

Naghdi

Fakhari

Bača

et al. 2020

Journal of Chromatography A

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Section: Chiral Selector Immobilization Via Physical Absorptionmentioning

confidence: 99%

“…However, α-hydroxy acids were better resolved on the dynamically coated phase [12]. Several authors have described the successful application of dynamically coated LC phase using different types of chiral selectors on a monolithic support or on packed columns [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous enantioseparation of nonsteroidal anti-inflammatory drugs by a one-dimensional liquid chromatography technique using a dynamically coated chiral porous silicon pillar array column

Naghdi

Fakhari

Bača

et al. 2020

Journal of Chromatography A

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“…For example, click chemistry can be used to prepare cyclodextrin‐based CSPs , in addition to the chemical incorporation of a chiral selector into the monolithic stationary phase (SP) . Dynamic coating is an alternative approach for SP preparation, which is characterized by efficiency, simplicity, and speed of preparation . Pittler et al.…”

Section: Introductionmentioning

confidence: 99%

The degree of substitution affects the enantioselectivity of sulfobutylether‐β‐cyclodextrin chiral stationary phases

et al. 2019

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Three chiral stationary phases were prepared by dynamic coating of sulfobutylether‐β‐cyclodextrin (SBE‐β‐CD) with different degrees of substitution, onto strong anion‐exchange stationary phases. The enantioselective potential and stability of newly prepared chiral stationary phases were examined using a set of structurally different chiral analytes. Measurements were performed in RP‐HPLC. Mobile phases consisted of methanol/formic acid, pH 2.10, and methanol/10 mM ammonium acetate buffer, pH 4.00, in various volume ratios. SBE‐β‐CDs with degrees of substitution (DS) 4, 6.3, and 10 proved suitable for the enantioseparation of 14, 11, and 8 analytes, respectively. The SBE‐β‐CD DS 4 based chiral stationary phase enabled the enantioseparation of the nearly all basic and neutral compounds. Chiral stationary phases with higher sulfobutylether‐β‐cyclodextrin substitution (especially DS 10) yielded higher enantioresolution values for acidic compounds.

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“…In addition, in the case of the polar group embedded alkyl phase, the polar group that is arranged close to the surface of silica reduces the silanol activity and improves separation of basic compound [12,13]. Furthermore, various organic phases, such as macrocyclic molecules [14,15] and helical oligoamides and -peptides [16,17], achieve specific selectivity by aligning functional groups based on their steric structure. We also found that highly ordered integration of weak interaction sites such as carbonyl groups can enhance the molecular shape selectivity of a stationary phase [18,19].…”

Section: Introductionmentioning

confidence: 99%

Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography

et al. 2016

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This paper introduces organic phases with aligned carbonyl groups derived from L-aspartic acid, L-glutamic acid, and L-α-aminoadipic acid; their stationary phases are denoted as Sil-Asp-2C n , Sil-Glu-2C n , and Sil-Adi-2C n , respectively. The stationary phases were used in high-performance liquid chromatography to investigate the alignment effect of carbonyl groups, which act as π-interaction sites, on molecular shape selectivity. The selectivities of the synthesized organic phases were evaluated using polyaromatic hydrocarbons (PAHs), including geometric isomers (e.g., cis-and trans-stilbenes). The PAH selectivities of the prepared stationary phases were higher than that of conventional octadecyl silica. Among the stationary phases prepared in this study, Sil-Glu-2C n (n = 18) showed the highest selectivity toward terphenyl isomers with different twist configurations and the lowest selectivity toward planar PAHs with different aspect ratios. The results show that the molecular shape selectivity of the phases was affected by the alignment of interaction sites. As a practical application of the octadecylated amino acid derivative-bonded stationary phases, we evaluated their selectivity for tocopherol isomers and achieved good separation. Furthermore, Sil-Asp-2C n (n = 1) showed hydrophilic interaction chromatography mode retention behavior for the separation of polar molecules like nucleosides.

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Chromatographic Characterization of a New Cationic β-CD Based Stationary Phase Prepared by Dynamic Coating

Cited by 8 publications

References 25 publications

Simultaneous enantioseparation of nonsteroidal anti-inflammatory drugs by a one-dimensional liquid chromatography technique using a dynamically coated chiral porous silicon pillar array column

Simultaneous enantioseparation of nonsteroidal anti-inflammatory drugs by a one-dimensional liquid chromatography technique using a dynamically coated chiral porous silicon pillar array column

The degree of substitution affects the enantioselectivity of sulfobutylether‐β‐cyclodextrin chiral stationary phases

Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography

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