Chromatographic characterization of triacylglycerides and fatty acid methyl esters in microalgae oils for biodiesel production

Soares, Aline Terra; Silva, B. F.; Fialho, Lucimar L.; Pequeno, Marcos Antonio Gomes; Vieira, Armando Augusto Henriques; Souza, A. G.; Filho, Nelson Roberto Antoniosi

doi:10.1063/1.4821289

Cited by 7 publications

(5 citation statements)

References 26 publications

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“…[15,45,47,49,55,58] [[50][51][52][53][54] Extubocellulus cf cribriger NCC229 France, NW Atlantic coast 4.8 ± 0.7 3.9 ± 0.5 9 ± 0 0.81 ± 0.09 33.7 ± 2.3 0.155 ± 0.003 14.2 ± 4.8 [52,59,60] Amphora sp. 1 NCC260 France, NW Atlantic coast 9.8 ± 1.3 3.7 ± 0.5 13 ± 0 0.78 ± 0.30 36.2 ± 2.6 0.176 ± 0.041 18.5 ± 4.2 [49,55,58,61,62] Conticriba weissflogii CCMP1336 USA, NE Atlantic coast 15.6 ± 2.5 10.8 ± 1.4 12 ± 0 0.59 ± 0.15 22.9 ± 2.6 0.277 ± 0.035 7.2 ± 1.7 -Brockmaniella brockmanii NCC161 France, NW Atlantic coast 9.2 ± 2.4 4.3 ± 0.6 10 ± 0 0.58 ± 0.01 37.5 ± 0.2 0.185 ± 0.025 3.7 ± 1.1 -Craspedostauros britannicus NCC195 France, NW Atlantic coast 37.8 ± 8.8 11.0 ± 2.2 9 ± 0 0.53 ± 0.09 44.7 ± 0.1 0.502 ± 0.039 8.2 ± 1.8 -Conticriba weissflogii NCC133 Morocco,Oum R'bia estuary 14.3 ± 1.7 12.1 ± 1.…”

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confidence: 99%

Lipids in benthic diatoms: A new suitable screening procedure

et al. 2019

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The selection of suitable and indigenous microalgae species is a fundamental requirement in developing added-value bioactive compounds recoverable in the food, health, and cosmetics markets. In this work, an integrated screening approach was developed to characterize the lipid rate of 33 diatom species (including 15 species studied for the first time) belonging to 16 genera from the Nantes Culture Collection, with the main objective of discovering bioactive lipid producers. For that purpose, a simple reliable method for establishing growth kinetics of strains and semi-quantitative analysis of lipid rates was developed. Growth kinetics measurements were achieved by daily minimal measurement fluorescence (F0) whereas lipid rate analyses were performed by high-throughput Fourier Transform Infrared spectroscopy on entire cells and lipid extracts. Results indicated that the method could be used directly on entire cells in spite of the presence of silica for the FTIR approach (due to frustule). The total lipid rate was species-dependant and ranged from 3.7% to 30.5% DW. Six strains out of 33 were found to present a higher total lipid rate superior to 15% DW, and 11 showed medium lipid rates ranging from 10% to 15% DW. The results revealed that five diatom species i.e. Amphora sp. NCC169, Nitzschia sp. NCC109, Nitzschia alexandrina NCC33, Opephora sp NCC366 and Staurosira sp. NCC182 presented interesting growth capabilities and should be further investigated as potential sources for their original lipid rate.

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confidence: 99%

Lipids in benthic diatoms: A new suitable screening procedure

et al. 2019

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“…Considering the environmental sustainability, like biodiesel production, taking account that European legislation (European Committee, 2008) establishes a maximum of 12% for linoleic acid and 1% for polyunsaturated acids (PUFA) (EN 14214). In addition to linolenic acid, γ-linolenic acid (C16:3 cis 6,9,12), C16:3 and C17:3 may also be present in microalgae species (Soares et al., 2013; Menezes et al., 2016). Since these are similar to linolenic acid, they need to be taken into consideration.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, these microorganisms are rich in triacylglycerides, which can be converted into fatty acids methyl esters (FAME) that can be used as feedstock for biodiesel production. This renewable fuel is less polluting than petroleum diesel, being a eco-friendly fuel (Menezes et al., 2013; Soares et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The gas chromatography coupled to flame ionization detector (GC-FID) is a powerful tool to detect and characterize FAME. These substances are thermally stable (Soares et al, 2013(Soares et al, , 2014 and it is the most applied method for the FAME analysis in vegetables, fruits, animal and microorganisms (Cossignani et al, 2011;Menezes et al, 2016;Blasi et al, 2017;Gouveia et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of major carotenoids and fatty acid composition of freshwater microalgae

Soares

Costa

Vieira

et al. 2019

Heliyon

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Considering the nutraceutical properties, the high commercial value from pigments and essential lipids and the environmental sustainability, the purposes of this study were to assess the major carotenoids and fatty acids composition of nine microalgae species as a source of nutraceutical compounds and as fatty raw material for biodiesel production. The carotenoid and fatty acid content were analyzed by high performance liquid chromatography tandem mass spectrometry detection method with atmospheric pressure chemical ionization mode (HPLC/APCI-MS/MS) and by high resolution gas chromatography with flame ionization detector (GC-FID). For the carotenoid analysis, the developed method presented a rapid response, a good chromatographic separation, higher sensitivity and can provides more compounds information due the mass spectrum. Among the microalgae evaluated, Desmodesmus protuberans (10.3 mg g -1 ), Desmodesmus denticulatus var. linearis (8.43 mg g -1 ) and Chlamydomonas planctogloea (7.4 mg g -1 ) are good lutein sources. Coelastrum sphaericum (15.29 mg g -1 ) and Parachlorella kessleri (22.96 mg g -1 ) showed high astaxanthin content; the others microalgae species presents low carotenoid content. In addition, Chlorella zofingiensis provides high quantities of γ-linolenic acid (4.3%). Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) levels were lower than 1.1 %. Regarding for biodiesel production, the promising strains are Coelastrum sphaericum and Parachlorella kessleri .

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“…23 Soares e colaboradores (2013) utilizaram coluna capilar de caráter apolar (5%-fenil-95%-metilpolissiloxano) para caracterização de TAGs encontrados em microalgas, visando posterior utilização dos mesmos para produção de biodiesel. 26 Outros dois trabalhos levaram em consideração um importante aspecto discutido na literatura: o modo de injeção em GC para análise de TAGs. [29][30][31] As técnicas de injeção em GC utilizando colunas capilares podem ser divididas em injeção direta na coluna (on-column) e injeção em um vaporizador com ou sem divisão de fluxo (Split/splitless).…”

Section: Cromatografia Gasosa (Gc)unclassified

Methods for the Determination of Triacylglycerols Composition: A Review of the Past Decade

Melo¹,

Meneghetti²,

Meneghetti³

et al. 2020

Rev. Virtual Quim.

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Over the last years (2008-2019), the most employed and innovative techniques for the analysis of triacylglycerol composition are presented and discussed in this article. Determining the composition of these molecules is of great importance since they are the main components of naturally occurring oils and fats and are products of great industrial and commercial interest. The application of oils and fats is closely related to the composition of these triacylglycerols in terms of fatty acids, having the chromatographic analysis techniques as the main and most used for the determination of this composition, with reversed phase high performance liquid chromatography and others techniques derived from it being the most employed. Ao longo dos últimos anos (2008-2019), as técnicas mais empregadas e inovadoras para a análise da composição de triacilgliceróis são apresentadas e discutidas neste artigo. A determinação da composição destas moléculas é de grande importância, uma vez que as mesmas são os principais componentes de óleos e gorduras de origem natural e são produtos de grande interesse industrial e comercial. A aplicação de óleos e gorduras está intimamente relacionada à composição desses triacilgliceróis em termos de ácidos graxos, tendo as técnicas cromatográficas de análise, como as principais e mais utilizadas para a determinação dessa composição, sendo a cromatografia líquida de alta eficiência em fase reversa e outras técnicas derivadas desta as mais empregadas.

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Chromatographic characterization of triacylglycerides and fatty acid methyl esters in microalgae oils for biodiesel production

Cited by 7 publications

References 26 publications

Lipids in benthic diatoms: A new suitable screening procedure

Lipids in benthic diatoms: A new suitable screening procedure

Analysis of major carotenoids and fatty acid composition of freshwater microalgae

Methods for the Determination of Triacylglycerols Composition: A Review of the Past Decade

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