Chromatographic Profile and Redox-Modulating Capacity of Methanol Extract from Seeds of Ginkgo biloba L. Originating from Plovdiv Region in Bulgaria

Abstract

Oxidative stress underlies the pathogenesis of many diseases, which determines the interest in natural substances with antioxidant properties. Ginkgo biloba L. leaves are well known and widely used in the pharmaceutical industry, but the therapeutic properties of the seeds are less studied. This study aimed to identify the chromatographic profile and to evaluate the antioxidant properties of methanol extract from seeds of G. biloba (GBSE). In the GBSE, flavonoids and terpenes were found as terpenes predominated. The GBSE antioxidant capacity determined by 2,2 azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and 1-diphenyl-2-picrylhydrazyl (DPPH) methods were equal to 1.34% and 0.58% of the activity of reference substance Trolox, respectively. The results of the ferric reducing antioxidant power method showed that the effect of concentration 1 mg/mL (w/v) GBSE was equal to 7.418 mM FeSO4 used as a standard. The cupric reducing antioxidant capacity activity of the GBSE was found to be 215.39 µmol Trolox/g GBSE and is presented as Trolox equivalent. The metal chelation effect of 1 mg/mL (w/v) GBSE was equal to that obtained for 0.018 mM EDTA. In conclusion, GBSE showed a good ability to neutralize ABTS and DPPH radicals and could have a beneficial effect in pathological conditions with oxidative stress etiology.

Tissue-Specific Profiling of Biflavonoids in Ginkgo (Ginkgo biloba L.)

“…The flavonoids in ginkgo are being researched by many scientists. However, most studies focus on monomeric flavonoids and biflavonoids are often neglected [ 13 , 14 , 15 ], e.g., in the review article by Liu et al [ 16 ], which focuses on the advances in chemical analysis and quality control of flavonoids in ginkgo, the presence of biflavonoids is hardly mentioned. To fill this gap in our knowledge of ginkgo flavonoids, the aim of the present study was to establish a tissue-specific profile of biflavonoids in ginkgo.…”

Thermal fixation technologies affect phenolic profile, ginkgolides, bilobalide, product quality, and ginkgolic acids in Ginkgo biloba leaf tea

“…Control was still the highest in the DPPH result, and the lowest one was IPF. The therapeutic effects of GBLs are attributed to quercetin and isorhamnetin, according to Petrov et al (2022). Both have demonstrated outstanding antioxidant activity.…”

Ginkgo Biloba - A Plant with Valuable Biological Properties

“…Dietary supplements containing plant extracts are complex mixtures whose therapeutic effects are often attributed to the cumulative effects of several components with bioactive properties. In the G. biloba plant, several bioactive compounds were identified and quantified, such as polyphenolic, including flavonoids, and terpene compounds [3,[15][16][17][18][19][20] (Table 1).…”

“…Ginkgo biloba leaves are and widely used in the pharmaceutical industry, but the therapeutic properties of the seeds are less studied. One of our research [15] aimed to evaluate the antioxidant properties of methanol extract from G. biloba seeds (GBSE). In the GBSE, flavonoids and terpenes were found as terpenes predominated.…”