Chromatographic quantitation of plasma and erythrocyte pentosidine in diabetic and uremic subjects

Odetti, Patrizio; Fogarty, John; Sell, D. R.; Monnier, V. M.

doi:10.2337/diabetes.41.2.153

Cited by 93 publications

(54 citation statements)

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“…The estimated GFR was calculated using the Cockcroft-Gault formula [27]. Pentosidine was measured by liquid chromatography [28] and AGE using an ELISA [29]. MDA was measured with a liquid chromatography method [30].…”

Section: Methodsmentioning

confidence: 99%

Relationship between glyco-oxidation, antioxidant status and microalbuminuria in type 2 diabetic patients

et al. 2009

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Aims/hypothesis This study examined the relationship, if any, between glucose-induced oxidative stress, antioxidant status and microalbuminuria in patients with type 2 diabetes. Methods The study involved 99 consecutive type 2 diabetic patients (57 men, 42 women). Patients with persistent microalbuminuria were identified and the following variables evaluated: fasting plasma glucose, HbA 1c , malonyldialdehyde (MDA), pentosidine, AGE, the total radical-trapping antioxidant parameter (TRAP), vitamin E, creatinine, estimated GFR and lipid profile. Results Patients were divided into two groups, i.e. 37 individuals without microalbuminuria (AER <20 μg/min) and 62 with microalbuminuria (AER ≥20 μg/min). The following variables were significantly higher in patients with microalbuminuria than in those without microalbuminuria (mean ± SD): fasting plasma glucose 9.41±2.88 vs 8.19±1.93 mmol/l, p<0.05; HbA 1c 7.97±1.51 vs 7.39± 1.03%, p<0.05; MDA 1.18±0.35 vs 1.02±0.29 µmol/l, p< 0.05; pentosidine 98.5±24.6 vs 82.9±20.9 pmol/ml, p< 0.005; and AGE 13.2±4.8 vs 10.6±3.8 µg/mg protein, p< 0.01. However, vitamin E and TRAP did not differ between the two groups. Serum creatinine values and estimated GFR were similar in the two groups. Only in patients with microalbuminuria were significant linear correlations seen between AER and both oxidation (HbA 1c r=0.33, p<0.01; MDA r=0.59, p<0.001; pentosidine r=0.48, p<0.001; and AGE r=0.44, p<0.001) and antioxidation variables (vitamin E r=−0.55, p<0.001; TRAP r=−0.49, p<0.001). Considering all variables together, multiple regression revealed a correlation between microalbuminuria and vitamin E, TRAP, HbA 1c and MDA, but not pentosidine or AGE. Conclusions/interpretation Our data suggest that microalbuminuria in type 2 diabetic patients might be promoted by an insufficient counter-regulation of the antioxidant system in the event of increased glyco-oxidation/glycation.

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Section: Methodsmentioning

confidence: 99%

Relationship between glyco-oxidation, antioxidant status and microalbuminuria in type 2 diabetic patients

et al. 2009

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“…AGE that can form between ribose, lysine, and arginine (42). Metabolic syndrome is a risk factor for diabetes (35).…”

Section: 42%id/gmentioning

confidence: 99%

“…Metabolic syndrome is a risk factor for diabetes (35). Plasma levels of pentosidines are elevated 2.5-fold in diabetics, and serum pentosidine levels in type II diabetes are positively associated with vascular complications (42,43). Thus, decreased hepatic ribose salvage could lead to higher blood ribose levels, increased AGE formation, and enhanced vascular damage.…”

Section: 42%id/gmentioning

confidence: 99%

Positron emission tomography probe demonstrates a striking concentration of ribose salvage in the liver

Clark

Flores²,

Evdokimov³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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F]DFA to show that ribose preferentially accumulates in the liver, suggesting a striking tissue specificity for ribose metabolism. We demonstrate that solute carrier family 2, member 2 (also known as GLUT2), a glucose transporter expressed in the liver, is one ribose transporter, but we do not know if others exist. [ 18 F]DFA accumulation is attenuated in several mouse models of metabolic syndrome, suggesting an association between ribose salvage and glucose and lipid metabolism. These results describe a tool for studying ribose salvage and suggest that plasma ribose is preferentially metabolized in the liver. molecular imaging | sugar metabolism | Slc2a2

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“…After hydrolysis, HCl was evaporated with Modulyo lyophilizer (Edwards Alto Vuoto SpA, Milan, Italy); each sample was reconstituted in water containing 0.01 mol/l heptafluorobutyric acid (Pierce, Prodotti Gianni, Milan, Italy) and filtered through a 0.45-m pore diameter filter (Ultrafree MC; Millipore, Milan, Italy). Pentosidine was determined in HPLC following the method of Odetti et al (31). Morphology.…”

Section: Animalsmentioning

confidence: 99%

Comparative Trial of N-Acetyl-Cysteine, Taurine, and Oxerutin on Skin and Kidney Damage in Long-Term Experimental Diabetes

et al. 2003

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This study analyzes the effect of chronic treatment with different antioxidants (N-acetyl-cysteine [NAC], taurine, a combination of NAC and taurine, and oxerutin) on long-term experimental diabetes induced by streptozotocin in rats. Glycoxidative damage was evaluated in the skin; glomerular structural changes were studied with morphometry and immunohistochemistry. Oxerutin treatment and the combined NAC plus taurine treatment resulted in reduced accumulation of collagenlinked fluorescence in skin in comparison with untreated diabetic rats. All treatments except taurine reduced glomerular accumulation of N ⑀ -(carboxymethyl)lysine and protected against the increase in glomerular volume typical of diabetes; furthermore, the apoptosis rate was significantly decreased and the glomerular cell density was better preserved. Glycoxidative markers in the skin turned out to be good indicators of the glomerular condition. The findings that emerged from our study support the hypothesis that glomerular damage in diabetes can be prevented or at least attenuated by supplementation with specific antioxidants. Treatment with oxerutin and combined treatment with NAC plus taurine gave the most encouraging results, whereas the results of taurine-only treatment were either negligible or negative and therefore suggest caution in the use of this molecule in single-drug treatment courses. Diabetes 52: 499 -505, 2003

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Chromatographic quantitation of plasma and erythrocyte pentosidine in diabetic and uremic subjects

Cited by 93 publications

References 0 publications

Relationship between glyco-oxidation, antioxidant status and microalbuminuria in type 2 diabetic patients

Relationship between glyco-oxidation, antioxidant status and microalbuminuria in type 2 diabetic patients

Positron emission tomography probe demonstrates a striking concentration of ribose salvage in the liver

Comparative Trial of N-Acetyl-Cysteine, Taurine, and Oxerutin on Skin and Kidney Damage in Long-Term Experimental Diabetes

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