Chromatographic Separation and Fluorometric Measurement of Vitamin B6 Components in Aqueous Solutions

Vitamin B6 components in some meats, fish, dairy products, and commercial infant formulas

“…Procedures for separating and determining the B6 components in hydrolyzed food extracts are important and essential developments for the vitamin B6 assay. Methods have been described for using Dowex 50 ion exchange columns to separate the vitamin B6 components of hydrolyzed food extracts into individual fractions and assaying the separated components with 5. car/sbergensis (MacArthur and Lehmann, 1959;Polansky et al, 1964; Toepfer and Lehmann, 1961). Data obtained by these procedures compare well with those obtained by rat bioassay for total vitamin B6 in a few selected food samples (Toepfer et al, 1963).…”

Vitamin Value Assay, Comparison of Vitamin B6 Values of Selected Food Samples by Bioassay and Microbiological Assay

“…The chromatographic separation of food extracts into pyridoxine, pyridoxal, and pyridoxamine fractions, using an ion exchange column with Dowex 50 resin, has made it possible to assay for these three factors individually and to calculate the total vitamin B6 content of the food (3,6,7).…”

Vitamin Determination, Separation of Pyridoxine, Pyridoxal, and Pyridoxamine by a Sulfonic Acid Ion Exchange Resin

“…A relationship of vitamin Be to the metabolism of protein and fat has been established (3,20), and diets high in protein and fat-a pattern common in the American populationenhance the requirement for this vitamin. More recently, vitamin Be deficiency experimentally established in cats and rats evoked an increased excretion of endogenous oxalic acid and formation of calcium oxalate stones in the urinary tract (7,5).…”

“…Previously, chemical procedures were not sensitive enough to determine vitamin B6 in foods and tissues or depended on a preliminary differential destruction, rather than a physical separation (4). Recently, fluorometric determination of the three free forms or the lactone of their oxidation product, 4-pyridoxic acid, has been shown to be very sensitive (7). The preliminary chromatographic separation of the three forms reported by these workers required the application of several hot, high ionic strength buffers, however.…”