Chromatographic separation of betulinic and oleanolic acids

Design and optimization of JO‐IEX process for highly efficient quaternary separation of 5′‐ribonucleotides

“…The advantages have been reported by many review articles. 3,[11][12][13][14] However, in some separation systems such as fermentation broth, biomass hydrolysate, and others, there are usually more than two components needed to be separated. Thus, the application of SMB in threecomponent or more-component separation systems has become one of the research hotspots in the separation and purification fields.…”

“…Compared with the fixed‐bed operation mode, the SMB technology has obvious advantages in the separation of two‐component mixed solutions. The advantages have been reported by many review articles 3,11–14 . However, in some separation systems such as fermentation broth, biomass hydrolysate, and others, there are usually more than two components needed to be separated.…”

Enhanced separation of bioactive triterpenic acids with a triacontylsilyl silica gel adsorbent: From impulse and breakthrough experiments to the design of a simulated moving bed unit

“…Thus, an SMB process may be a potential candidate for TTAs separation. Several works in the literature focus methodologies for the chromatographic separation/isolation of compounds by SMB [32,[50][51][52].…”

“…A series of elution experiments were performed with the Acclaim C30 column with the purpose of finding a suitable mobile phase for the separation of oleanolic and ursolic acids. It is known that the retention of oleanolic and ursolic acids is highly dependent on mobile phase composition [52,[73][74][75][76], thus a careful analysis of the influence of each component in the eluent mixture is necessary to ensure that a good compromise between TTAs solubility and selectivity (and resolution) is achieved. In this work, methanol, water, acetonitrile, and mixtures thereof were used because: (i) they allow simple TTAs UV-vis detection; (ii) they do not increase significantly the pressure drop as in the case of more viscous solvents (ethanol, isopropanol, n-butanol, etc.…”

Purification of rutin by supercritical fluid simulated moving bed chromatography

“…[20] In the early 1960s, the UOP Company first applied SMB technology to the separation of xylene in the petrochemical production process, and currently SMB is widely used in the sugar industry [21] and in separation of natural products and fine chemicals. [22] Azenha et al [23] obtained raffinate stream with a purity of 99.0% for betulinic and oleanolic acid in the extract stream with 99.4% purity. Li et al [24] investigated the feasibility of xylo-oligosaccharides purification by sequential simulated moving bed (SSMB).…”