Chromatographic Separation of Corticoids on a Thin Layer of Silica Gel

TLC of Steroids and Related Compounds

“…(toluenesulphonate) > -OCOCH a but in 120: it is the other way round [2]. 20:,3P-Diols shows themselves to be more polar than 3p,17 p-diols; 1 p,3 p-diols correspond, however, only to a monohydroxy-compound.…”

“…Oorticosteroids from urine or tissue extracts, whether in unchanged or metabolised form, have proved to be a profitable class for the application of TLC [2,3,19,31,123,133,138,190,191,194,205,206,217] which competes here with .PC. The instability of the corticosteroids must be remembered when using adsorption TLC [123,138].…”

“…The instability of the corticosteroids must be remembered when using adsorption TLC [123,138]. ADAMEO et al [2,3] have carried out separations using 1, distilled water (preliminary purification), 2. methylene dichloride-ethanol (95 + 5) and 3. chloroformethanol (93 + 7) in the same direction on the same silica gel G layer. They then localised the zones with a very thin coating of blue tetrazolium and analysed the eluates from these zones quantitatively according to the PORTER-SILBER method.…”