1977
DOI: 10.1128/jcm.6.3.238-243.1977
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Chromatographic Separations of Alphavirus Strains by Hydroxylapatite

Abstract: Hydroxylapatite column chromatography methods were developed to characterize selected alphavirus populations. Different conditions of pH and phosphate molarity were required to obtain satisfactory elution profiles and separations for Western equine encephalomyelitis virus strains, compared with Eastern equine encephalomyelitis virus and Semliki Forest virus strains. Raising the pH of the buffers effected earlier elutions of all viruses. Selection of phosphate gradients with more gentle slopes and adjustment to… Show more

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Cited by 6 publications
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