Chromatographic test methods for characterizing alkylsiloxane-bonded silica columns for reversed-phase liquid chromatography

Poole, Colin F.

doi:10.1016/j.jchromb.2018.06.011

Cited by 42 publications

(9 citation statements)

References 95 publications

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“…A number of different sample characteristics and purification principles must be considered when selecting a 2D combination approach for sample purification to achieve the highest peak capacity [26–28]. Relative to other 2D combinations, an RPLC/RPLC 2D approach is better suited to separating nonpolar, polar and ionic compounds [29, 30]. The PTAS column filler exhibited typical RPLC retention characteristics, which showed good separation selectivity for hydrophobic compounds [31].…”

Section: Resultsmentioning

confidence: 99%

Preparative isolation of 1,1‐diphenyl‐2‐picrylhydrazyl inhibitors from Ribes himalense using medium‐pressure and two‐dimensional reversed‐phase/reversed‐phase liquid chromatography guided by an online HPLC‐1, 1‐diphenyl‐2‐picrylhydrazyl assay

LIU

Lei

Dang

et al. 2021

J of Separation Science

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The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical‐scavenging effects. In the present work, we utilized it as a model herb to highlight an approach for the separation of 1,1‐diphenyl‐2‐picrylhydrazyl inhibitors via medium‐pressure chromatography and two‐dimensional reversed‐phase/reversed‐phase interaction liquid chromatography under the guidance of an online high‐performance liquid chromatography‐1,1‐diphenyl‐2‐picrylhydrazyl assay. Finally, we obtained two free radical inhibitors (>95% purity) from the R. himalense extract. This is the first report of the rapid isolation of these free radical inhibitors from R. himalense. This method can be useful in quality standard assessment and further pharmacological activity research, and may be used as a reference for the composition research of various natural products.

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Section: Resultsmentioning

confidence: 99%

Preparative isolation of 1,1‐diphenyl‐2‐picrylhydrazyl inhibitors from Ribes himalense using medium‐pressure and two‐dimensional reversed‐phase/reversed‐phase liquid chromatography guided by an online HPLC‐1, 1‐diphenyl‐2‐picrylhydrazyl assay

LIU

Lei

Dang

et al. 2021

J of Separation Science

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“…Polyamide, which allows the separation to take place based on the formation of kinds of hydrogen bonds, is mainly used for the separation of phenols, quinones, flavonoids, anthraquinones, tannins, etc. [17].…”

Section: Adsorption Chromatographymentioning

confidence: 99%

Analytical Methods of Isolation and Identification

Feng¹,

Li²,

Hao³

et al. 2020

Phytochemicals in Human Health

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The chemical constituents of plants are complicated, and monomeric compounds must be obtained via extraction and isolation before structure identification, bioactivity screening, and so on. In recent years, the new technologies and methods of the extraction, isolation, and structural identification have come forth, which promote the speed of extraction and analysis of phytochemicals. The chemical structures of compounds from plants must be identified or elucidated, which may provide the necessary basis for further study on the bioactivities, structureactivity relationships, metabolisms in vivo, structural modification, and synthesis of the active compounds. The amount of chemical constituents isolated from plants is often minor, so the structural studies are often difficult to carry out with classical methods. Therefore, spectral analysis is mainly used. This chapter describes the isolation and identification methods during the study of phytochemicals.

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“…Surface coverage of a typical C18 ligand span between 4 and 4.5 micromoles/m 2 , while the Separations 2021, 8, 59 2 of 12 surface concentration of silanols on bare silica is about 8.0 micromoles/m 2 [7]. Moreover, the additional "end-capping" procedure, with smaller silylating agents, fails to inactivate the residual silanols [8]. In the last 20 years, with the diffusion of ultra-high-pressure liquid chromatography (UHPLC), a lot of attention has been paid to innovative silica bulks with a reduced number of silanols.…”

Section: Introductionmentioning

confidence: 99%

Static vs. Dynamic Electrostatic Repulsion Reversed Phase Liquid Chromatography: Solutions for Pharmaceutical and Biopharmaceutical Basic Compounds

et al. 2021

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Many efforts have been made to separate basic compounds, which are challenging to resolve in reversed phase liquid chromatography. In this process, they are strongly retained and the peak shape undergoes significant distortion. The principal origin of this has been identified with the non-negligible interaction with residual deprotonated silanols. Consequently, all solutions that efficiently shield silanols are being sought. This review is an upgrade on the use of the electrostatic repulsion reversed phase (ERRP) approach: retention of bases, in protonated form, can be achieved by modulating the charge repulsion caused by the presence of positive charges in the chromatographic system. This study successfully (i) introduced fixed positive charges in the structure of stationary phases, (ii) used cationic and hydrophobic additives in the mobile phase, and (iii) used the ERRP-like approach employed at the preparative level for peptide purification.

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Chromatographic test methods for characterizing alkylsiloxane-bonded silica columns for reversed-phase liquid chromatography

Cited by 42 publications

References 95 publications

Preparative isolation of 1,1‐diphenyl‐2‐picrylhydrazyl inhibitors from Ribes himalense using medium‐pressure and two‐dimensional reversed‐phase/reversed‐phase liquid chromatography guided by an online HPLC‐1, 1‐diphenyl‐2‐picrylhydrazyl assay

Preparative isolation of 1,1‐diphenyl‐2‐picrylhydrazyl inhibitors from Ribes himalense using medium‐pressure and two‐dimensional reversed‐phase/reversed‐phase liquid chromatography guided by an online HPLC‐1, 1‐diphenyl‐2‐picrylhydrazyl assay

Analytical Methods of Isolation and Identification

Static vs. Dynamic Electrostatic Repulsion Reversed Phase Liquid Chromatography: Solutions for Pharmaceutical and Biopharmaceutical Basic Compounds

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