Chromatography of Tobacco Mosaic Virus on a Cellulose Cation Exchange Adsorbent

Cochran, George W.; Chidester, John L.; Stocks, Dayna L.

doi:10.1038/1801281a0

Cited by 11 publications

(3 citation statements)

References 3 publications

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“…Bind/Elute chromatography is the main chromatographic method employed for virus purification (Table 1). Early chromatography media for tobamovirus enrichment included cellulose derivatives [289][290][291]. With the advent of monolithic columns [292,293], tobamoviruses found compatibility with supports fashioned as continuous homogeneous phases [292,294,295].…”

Section: │5│2 Bind/elute Chromatographymentioning

confidence: 99%

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Preprint

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This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages, and presents a novel efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses: from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. Best practice and also rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls and 'forgotten' or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of the multivalent soft-matter nanorods.

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Section: │5│2 Bind/elute Chromatographymentioning

confidence: 99%

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Bind/elute chromatography is the main chromatographic method employed for virus purification ( Table 1 ). Early chromatography media for tobamovirus enrichment included cellulose derivatives [ 298 , 299 , 300 ]. With the advent of monolithic columns [ 301 , 302 ], tobamoviruses found compatibility with supports fashioned as continuous homogeneous phases [ 301 , 303 , 304 ].…”

Section: Techniques For Virion Enrichment and Storage One By Onementioning

confidence: 99%

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

Wendlandt,

Britz,

Kleinow

et al. 2024

Viruses

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This article develops a multi-perspective view on motivations and methods for tobamovirus purification through the ages and presents a novel, efficient, easy-to-use approach that can be well-adapted to different species of native and functionalized virions. We survey the various driving forces prompting researchers to enrich tobamoviruses, from the search for the causative agents of mosaic diseases in plants to their increasing recognition as versatile nanocarriers in biomedical and engineering applications. The best practices and rarely applied options for the serial processing steps required for successful isolation of tobamoviruses are then reviewed. Adaptations for distinct particle species, pitfalls, and ‘forgotten’ or underrepresented technologies are considered as well. The article is topped off with our own development of a method for virion preparation, rooted in historical protocols. It combines selective re-solubilization of polyethylene glycol (PEG) virion raw precipitates with density step gradient centrifugation in biocompatible iodixanol formulations, yielding ready-to-use particle suspensions. This newly established protocol and some considerations for perhaps worthwhile further developments could serve as putative stepping stones towards preparation procedures appropriate for routine practical uses of these multivalent soft-matter nanorods.

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“…111 1953, Putnam ( 5 ) reviewed the literature dealing with reversible adsorption as a means of purifying bacteriophage and expressed the hope that improvements in chromatographic methods might lead to developmeilt of a rapid and simple method for phage purification. Since then there have been a number of reports (9,11) on the chromatography of bacteriophages and, in addition, chromatographic methods have been widely applied to the p~~rification and concentration of animal (4,7,8) and plant (2) viruses.…”

Section: Introductionmentioning

confidence: 99%

CHROMATOGRAPHY OF COLIPHAGES T₁ AND T₂ ON COLUMNS OF CALCIUM PHOSPHATE

Robinson¹,

Douglas²,

I³

et al. 1960

Can. J. Microbiol.

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Coliphages T I and T? were efficiently adsorbed from dialyzed lysates by small columns of calcium phosphate. Elution of the columns with phosphate buffers a t low ~nolarity (0.02-0.03) removed much of the non-infectious material; the phage could subsequently be eluted ill more concentrated and purified form with higher buffer concentrations (0.06 ill or greater). The chromatographic behavior of T I and TI is contrasted and the influence of non-phage constituents of the lysate upon the chro~natographic separation is discussed.

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Chromatography of Tobacco Mosaic Virus on a Cellulose Cation Exchange Adsorbent

Cited by 11 publications

References 3 publications

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

CHROMATOGRAPHY OF COLIPHAGES T₁ AND T₂ ON COLUMNS OF CALCIUM PHOSPHATE

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Chromatography of Tobacco Mosaic Virus on a Cellulose Cation Exchange Adsorbent

Cited by 11 publications

References 3 publications

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle – Why and How? Purification Routes over Time and a New Customizable Approach

Getting Hold of the Tobamovirus Particle—Why and How? Purification Routes over Time and a New Customizable Approach

CHROMATOGRAPHY OF COLIPHAGES T1 AND T2 ON COLUMNS OF CALCIUM PHOSPHATE

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CHROMATOGRAPHY OF COLIPHAGES T₁ AND T₂ ON COLUMNS OF CALCIUM PHOSPHATE