Chromium Monitoring in Water by Colorimetry Using Optimised 1,5-Diphenylcarbazide Method

Abstract

Chromium contamination of drinking water has become a global problem due to its extensive use in industry. The most commonly used methods for chromium detection in water are laboratory-based methods, such as atomic absorption spectroscopy and mass spectroscopy. Although these methods are highly selective and sensitive, they require expensive maintenance and highly trained staff. Therefore, there is a growing demand for cost effective and portable detection methods that would meet the demand for mass monitoring. Microfluidic detection systems based on optical detection have great potential for onsite monitoring applications. Furthermore, their small size enables rapid sample throughput and minimises both reagent consumption and waste generation. In contrast to standard laboratory methods, there is also no requirement for sample transport and storage. The aim of this study is to optimise a colorimetric method based on 1,5-diphenylcarbazide dye for incorporation into a microfluidic detection system. Rapid colour development was observed after the addition of the dye and samples were measured at 543 nm. Beer’s law was obeyed in the range between 0.03–3 mg·L−1. The detection limit and quantitation limit were found to be 0.023 and 0.076 mg·L−1, respectively.

Utilization of Tires Waste-Derived Magnetic-Activated Carbon for the Removal of Hexavalent Chromium from Wastewater

“…The concentration of Cr was analyzed in waster sample through UV spectrophotometer (Shimadzu A90 UV-visible spectrophotometer; Shimadzu, Kyoto, Japan) using DPC method [ 26 ]. Diphenyl carbazide (DPC; Sigma-Aldrich, St. Louis, MO, USA) solution was prepared by dissolving 0.25 g DPC in 50 mL of acetone, which is used for complexation of Cr ions in this method.…”

Bioprocessing strategies for cost-effective simultaneous removal of chromium and malachite green by marine alga Enteromorpha intestinalis

“…According to the World Health Organization (WHO), the maximum permissible limits of Cr(VI) in drinking water is 0.05 mg/L 14 . Chromium cannot be biodegraded easily and therefore chromium exceeds the permissible limit accumulated in the food chain and become destructive to human health.…”

Draft genome sequence data of a chromium reducing bacterium, Bacillus licheniformis strain KNP

“…All eighteen bacteria were screened for the reduction of hexavalent chromium at various concentrations (200–1200 ppm) of potassium dichromate by the diphenylcarbazide colorimetric method [10] . Out of eighteen bacteria, only one bacterium designated strain KNP showed the hexavalent chromium reduction up to concentrations of 1000 ppm.…”