Chromium Tolerance and Reduction Potential of a Bacillus sp.ev3 Isolated from Metal Contaminated Wastewater

Abstract

This study was aimed at assessing the ability of Bacillus sp.ev3 to reduce hexavalent chromium into its trivalent form. Bacillus sp.ev3 could tolerate Cr(6+) (4800 microg/mL), Pb(2+) (800 microg/mL), Cu(2+) (200 microg/mL), Cd(2+) (50 microg/mL), Zn(2+) (400 microg/mL), Ni(2+) (4000 microg/mL) and Hg(2+) (50 microg/mL). Bacillus sp.ev3 showed optimum growth at 37 degrees C and pH at 7. Bacillus sp.ev3 could reduce 91% of chromium from the medium after 96 h and was also capable to reduce 84% chromium from the industrial effluents after 144 h. Cell free extracts of Bacillus sp.ev3 grown in the presence of Cr showed reduction of 70%, 45.6% and 27.4% at concentrations of 10 microg Cr(6+)/mL, 50 microg Cr(6+)/mL and 100 microg Cr(6+)/mL, respectively.

Assessment of Present Heavy Metals in Industrial Affected Soil Area of Mandideep, Madhya Pradesh, India

“…Wastewaters from the industries and sewage sludge applications have permanent toxic effects to human and the environment (Rehman et al, 2008) Cadmium is one of the most toxic pollutants of the surface soil layer, released into the environment by mining and smelting activities, atmospheric deposition from metallurgical industries, incineration of plastics and batteries, land application of sewage sludge, and burning of fossil fuels (Tang et al, 2006). Nickel (Ni) is the 24th most abundant element in the earth crust and has been detected indifferent media in all parts of the biosphere.…”

Isolation and Growth Characteristics of Chromium(VI) and Pentachlorophenol Tolerant Bacterial Isolate from Treated Tannery Effluent for its Possible Use in Simultaneous Bioremediation

“…Several bacteria such as Pseudomonas ambigua [6], Bacillus sp. [7,8], Serratia marcescens [9] have been reported for Cr(VI) reduction. Serious concern about the toxicity of Cr compounds necessitates recovery and reuse of Cr from tannery effluent as well as other industrial wastes and/or rendering it to a less toxic form [10].…”

Isolation and Identification of Biosurfactant Producing Chromate Resistant Bacteria Isolated From Chromium Contaminated Site.

“…This study highlights the prevalent occurrence of Cr(VI) resistant bacterial population in the soil of dumping site of battery industry waste. It also reveals that continuous exposure to high levels of Cr(VI) may result in bacterial community that have an exceptional ability to adapt in chromate polluted environments and might have developed some detoxification mechanism as an effective tool for survival in the stress environments (Rehman et al, 2008).…”

“…The waste discharged from the battery manufacturing industries contains various chemicals including chromium sulfate in large amount which is ultimately released into the cultivable lands and nearby water bodies beyond the permissible limit (Rehman et al, 2008). The predominant forms of chromium present in environment are the trivalent [Cr(III)] and hexavalent chromium [Cr(VI), chromate].…”